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Single Nucleotide Polymorphisms / Methods and Protocols, Kwok Pui-Yan

Single Nucleotide Polymorphisms / Methods and Protocols

Автор: Kwok Pui-Yan
Название:  Single Nucleotide Polymorphisms / Methods and Protocols   (Полиморфизм нуклеотида / методы и протоколы)
Издательство: Springer
Медицинская генетика

ISBN: 0896039684
ISBN-13(EAN): 9780896039681
ISBN: 0-89603-968-4
ISBN-13(EAN): 978-0-89603-968-1
Обложка/Формат: Hardcover
Страницы: 288
Вес: 0.593 кг.
Дата издания: 2003
Серия: Methods in Molecular Biology
Язык: ENG
Размер: 23.52 x 15.85 x 2.08 cm
Поставляется из: Германии
Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Дополнительное описание: Формат: 229x152
Илюстрации: 60
Круг читателей: Researchers interested in genetic studies, including association studies of common diseases in humans, DNA diagnostics, forensics, and agricultural biotechnology
Ключевые слова:
Язык: eng

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Single Nucleotide Polymorphisms

Автор: Komar
Название: Single Nucleotide Polymorphisms
ISBN: 1603274103 ISBN-13(EAN): 9781603274104
Издательство: Springer
Цена: 12316 р.
Наличие на складе: Нет в наличии.

Single Cell Diagnostics / Methods and Protocols

Автор: Thornhill Alan
Название: Single Cell Diagnostics / Methods and Protocols
ISBN: 1588295788 ISBN-13(EAN): 9781588295781
Издательство: Springer
Цена: 7036 р.
Наличие на складе: Нет в наличии.
Описание: Single Cell Diagnostics: Methods and Protocols applies modernmolecular diagnostic techniques to the analysis of single cells, smallnumbers of cells, or cell extracts. Emphasis is placed on non-invasiveanalysis of single cell metabolites and the direct analysis of RNA andDNA from single cells, with a focus on polymerase chain reaction andfluorescence in situ hybridization. The authors, all leadingscientists in the field, provide current and innovative methods forextracting useful, accurate, and reliable data from single-cellsamples. The stepwise, technically precise protocols presented in thisvolume thoroughly describe single-cell assays, from material collectionand isolation of cells to data interpretation. Cell-types analyzedinclude embryonic blastomeres, fibroblasts, and lymphocytes. Among thetechniques described are real-time quantitative PCR, isothermal wholegenome amplification, comparative genomic hybridization, real-timegenetic expression analysis, and the production of RNA and cDNAlibraries. This handbook will be essential for researchers andclinicians, and practitioners providing care for couples seekingtreatment for infertility or preimplantation genetic diagnosis.

Cytokine Gene Polymorphisms in Multifactorial Conditions

Автор: Koen Vandenbroeck
Название: Cytokine Gene Polymorphisms in Multifactorial Conditions
ISBN: 0849336198 ISBN-13(EAN): 9780849336195
Издательство: Taylor&Francis
Цена: 10120 р.
Наличие на складе: Нет в наличии.
Описание: Written by the leading specialists, this book brings together, reviews, and structures up-to-date information on polymorphisms in cytokine genes. While the beginning chapters discuss the properties of individual cytokines, in the later chapters cytokines are re-presented considering the individual disease or condition. Broadening understanding of the effect of genetic variations on human immune responses, the organization, scope, and content of this book make it a valuable and easily accessible resource. A reference for geneticists, immunologists, cell biologists and clinicians, the book is a must for everyone involved in, or planning, cytokine genetics or immunogenetics studies.

Human Nucleotide Expansion Disorders

Автор: Fry Michael, Usdin Karen
Название: Human Nucleotide Expansion Disorders
ISBN: 3540333355 ISBN-13(EAN): 9783540333357
Издательство: Springer
Цена: 21032 р.
Наличие на складе: Нет в наличии.
Описание: Human neurological and neuromuscular disorders caused by nucleotide expansion, first discovered in 1991, are the focus of growing interest of practicing physicians and of interested biomedical researchers. This volume represents a comprehensive and up-to-date description of many of the better-studied disorders. The expert authors discuss molecular, clinical and pathological aspects of the diseases as well as our current understanding of their underlying mechanisms. Of special interest are ideas and initial results of the different therapeutic strategies that can be employed to overcome some of the disorders. As a summary of the state-of-the-art research in this field, this book is of value to human geneticists, molecular biologists and biochemists as well as to practicing neurologists and pediatricians.

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