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RT-PCR Protocols, Connell Joe

RT-PCR Protocols

Автор: Connell Joe
Название:  RT-PCR Protocols   (Протоколы RT-PCR в реальном времени)
Издательство: Springer
Клеточная биология

ISBN: 0896038750
ISBN-13(EAN): 9780896038752
ISBN: 0-89603-875-0
ISBN-13(EAN): 978-0-89603-875-2
Обложка/Формат: Hardcover
Страницы: 380
Вес: 0.752 кг.
Дата издания: 2002
Серия: Methods in Molecular Biology
Язык: ENG
Размер: 23.42 x 15.49 x 2.67 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
Дополнительное описание: Формат: 229x152
Илюстрации: 100
Круг читателей: Biomedical researchers and molecular biologists working in university laboratories, hospital research and diagnostic laboratories, and in the biotechnology and pharmaceutical industries
Ключевые слова:
Язык: eng

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Recombinant Gene Expression / Reviews and Protocols

Автор: Balbas Paulina, Lorence Argelia
Название: Recombinant Gene Expression / Reviews and Protocols
ISBN: 1588292622 ISBN-13(EAN): 9781588292629
Издательство: Springer
Цена: 8852 р.
Наличие на складе: Нет в наличии.
Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Цена: 6157 р.
Наличие на складе: Есть (3 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Antibiotic Resistance Protocols

Автор: Steven H. Gillespie; Timothy D. McHugh (Eds.)
Название: Antibiotic Resistance Protocols
ISBN: 160327278X ISBN-13(EAN): 9781603272780
Издательство: Springer
Цена: 7697 р.
Наличие на складе: Есть (1 шт.)

Human Embryonic Stem Cell Protocols

Автор: Turksen
Название: Human Embryonic Stem Cell Protocols
ISBN: 1607613689 ISBN-13(EAN): 9781607613688
Издательство: Springer
Цена: 7697 р.
Наличие на складе: Есть (1 шт.)

Matrix Metalloproteinase Protocols

Автор: Ian M. Clark (Ed.)
Название: Matrix Metalloproteinase Protocols
ISBN: 1603272984 ISBN-13(EAN): 9781603272988
Издательство: Springer
Цена: 9237 р.
Наличие на складе: Есть (1 шт.)
Описание: Detailing proven laboratory techniques for the study of MMPs, this book covers the methods including those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances.

In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
Цена: 1092 р.
Наличие на складе: Есть (4 шт.)
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Adipose tissue protocols

Название: Adipose tissue protocols
ISBN: 1588299163 ISBN-13(EAN): 9781588299161
Издательство: Springer
Цена: 6157 р.
Наличие на складе: Нет в наличии.

Reviews and Protocols in DT40 Research / Subcellular Biochemistry

Автор: Buerstedde Jean-Marie, Takeda Shunichi
Название: Reviews and Protocols in DT40 Research / Subcellular Biochemistry
ISBN: 1402048955 ISBN-13(EAN): 9781402048951
Издательство: Springer
Цена: 18403 р.
Наличие на складе: Нет в наличии.
Описание: Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard.

2D PAGE / Volume 2: Applications and Protocols

Автор: Posch Anton
Название: 2D PAGE / Volume 2: Applications and Protocols
ISBN: 1603272097 ISBN-13(EAN): 9781603272094
Издательство: Springer
Цена: 7158 р.
Наличие на складе: Нет в наличии.
Описание: This book presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, is still a fundamental research tool for nearly all protein-related scientific projects. The methods described here in detail are not limited to 2-DE and can also be applied to other protein separation techniques.

Adenovirus Methods and Protocols / Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models

Автор: Wold William S. M., Tollefson Ann E.
Название: Adenovirus Methods and Protocols / Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models
ISBN: 1588295982 ISBN-13(EAN): 9781588295989
Издательство: Springer
Цена: 6157 р.
Наличие на складе: Нет в наличии.
Описание: Designed to help new researchers to conduct studies involving adenoviruses and to help established researchers to branch into new areas. This work features topics that include deletion mutants, capsid modifications, insertions, and gene replacements both in human adenoviruses and murine, bovine, and ovine adenoviruses.

Adhesion Protein Protocols

Автор: Coutts Amanda S.
Название: Adhesion Protein Protocols
ISBN: 1588295338 ISBN-13(EAN): 9781588295330
Издательство: Springer
Цена: 6157 р.
Наличие на складе: Нет в наличии.
Описание: The second edition of Adhesion Protein Protocols combines traditionaltechniques with cutting-edge and novel techniques that can be adaptedeasily to different molecules and cell types. The topics discussed inthis updated second edition include novel techniques for studyingcell-cell adhesion, neutrophil chemotaxis, in vitro assays used tostudy leukocyte migration through monolayers of cultured endothelialcells, and novel techniques to purify pseudopodia from migratory cells.This book also discusses the study of cell-matrix interaction, RNAinterference, fluorescence recovery after photobleaching, actinpurification, the application of microarray techniques, and the role ofadhesion proteins in the study of proteomics.The protocols discussed in this volume are suitable for both novice andexpert scientists, who will gain further insight into the complex andincompletely understood processes involved in cellular adhesion.

PRINS and In Situ PCR Protocols

Автор: Pellestor Franck
Название: PRINS and In Situ PCR Protocols
ISBN: 1588295494 ISBN-13(EAN): 9781588295491
Издательство: Springer
Цена: 5926 р.
Наличие на складе: Нет в наличии.
Описание: This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.

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