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RT-PCR Protocols, Connell Joe

RT-PCR Protocols

Автор: Connell Joe
Название:  RT-PCR Protocols   (Протоколы RT-PCR в реальном времени)
Издательство: Springer
Классификация:
Клеточная биология

ISBN: 0896038750
ISBN-13(EAN): 9780896038752
ISBN: 0-89603-875-0
ISBN-13(EAN): 978-0-89603-875-2
Обложка/Формат: Hardcover
Страницы: 380
Вес: 0.752 кг.
Дата издания: 2002
Серия: Methods in Molecular Biology
Язык: ENG
Размер: 23.42 x 15.49 x 2.67 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
Дополнительное описание: Формат: 229x152
Илюстрации: 100
Круг читателей: Biomedical researchers and molecular biologists working in university laboratories, hospital research and diagnostic laboratories, and in the biotechnology and pharmaceutical industries
Ключевые слова:
Язык: eng



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Recombinant Gene Expression / Reviews and Protocols

Автор: Balbas Paulina, Lorence Argelia
Название: Recombinant Gene Expression / Reviews and Protocols
ISBN: 1588292622 ISBN-13(EAN): 9781588292629
Издательство: Springer
Цена: 8851 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.
Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.
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Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Цена: 6156 р.
Наличие на складе: Есть (3 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
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Histology Protocols

Автор: Tim D. Hewitson; Ian A. Darby (Eds.)
Название: Histology Protocols
ISBN: 1603273441 ISBN-13(EAN): 9781603273442
Издательство: Springer
Цена: 5902 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.
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Species Diagnostics Protocols / PCR and Other Nucleic Acid Methods

Автор: Clapp Justin P.
Название: Species Diagnostics Protocols / PCR and Other Nucleic Acid Methods
ISBN: 0896033236 ISBN-13(EAN): 9780896033238
Издательство: Springer
Цена: 8466 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.
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Clinical Applications of PCR

Автор: Lo Y. M. Dennis
Название: Clinical Applications of PCR
ISBN: 1588293483 ISBN-13(EAN): 9781588293480
Издательство: Springer
Цена: 6541 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.
Описание: In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.
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PCR Cloning Protocols

Автор: Chen Bing-Yuan, Janes Harry W.
Название: PCR Cloning Protocols
ISBN: 0896039730 ISBN-13(EAN): 9780896039735
Издательство: Springer
Цена: 8466 р.
Наличие на складе: Нет в наличии.
Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
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PRINS and In Situ PCR Protocols

Автор: Pellestor Franck
Название: PRINS and In Situ PCR Protocols
ISBN: 1588295494 ISBN-13(EAN): 9781588295491
Издательство: Springer
Цена: 5925 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.
Описание: This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.
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PCR Mutation Detection Protocols

Автор: Theophilus Bimal D. M., Rapley Ralph
Название: PCR Mutation Detection Protocols
ISBN: 0896036170 ISBN-13(EAN): 9780896036178
Издательство: Springer
Цена: 5617 р.
Наличие на складе: Нет в наличии.
Описание: Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.
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PCR Cloning Protocols

Автор: Chen Bing-Yuan, Janes Harry W.
Название: PCR Cloning Protocols
ISBN: 0896039692 ISBN-13(EAN): 9780896039698
Издательство: Springer
Цена: 8466 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.
Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
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PCR Sequencing Protocols

Автор: Rapley Ralph
Название: PCR Sequencing Protocols
ISBN: 0896033449 ISBN-13(EAN): 9780896033443
Издательство: Springer
Цена: 5617 р.
Наличие на складе: Нет в наличии.
Описание: A superb, comprehensive collection of the most reliable protocols for PCR sequencing-all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.
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PCR Protocols on CD (Institutional)

Автор: Walker John M.
Название: PCR Protocols on CD (Institutional)
ISBN: 1588294099 ISBN-13(EAN): 9781588294098
Издательство: Springer
Цена: 18403 р.
Наличие на складе: Нет в наличии.
Описание: In the tradition of the acclaimed Methods in Molecular Biology series, this collection of more than 500 tried-and-tested PCR protocols will become the standard PCR reference set in all biological science laboratories. Comprehensive in scope, yet easily searchable, these protocols will enable PCR labs to have access to the widest array of PCR protocols available and thus be able to complete any kind of PCR experiment with ease. The protocols are presented in a step-by-step fashion and cover every aspect of PCR, making this an essential reference for all biological scientists.
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PCR Protocols

Автор: Bartlett John M.S., Stirling David
Название: PCR Protocols
ISBN: 0896036278 ISBN-13(EAN): 9780896036277
Издательство: Springer
Цена: 7465 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.
Описание: Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly since the first edition, and has expanded its use in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The meethods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR techinque in their work.
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