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In Situ Detection of DNA Damage / Methods and Protocols, Didenko Vladimir V.

In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название:  In Situ Detection of DNA Damage / Methods and Protocols   (Внутреннее обнаружение повреждений ДНК, методы и протоколы)
Издательство: Springer
Классификация:
Медицинская генетика
Клеточная биология

ISBN: 0896039528
ISBN-13(EAN): 9780896039520
ISBN: 0-89603-952-8
ISBN-13(EAN): 978-0-89603-952-0
Обложка/Формат: Hardback
Страницы: 336
Вес: 0.721 кг.
Дата издания: 2002
Серия: Methods in Molecular Biology
Язык: ENG
Иллюстрации: 133 black & white illustrations, 31 colour illustrations, biography
Размер: 23.47 x 16.10 x 2.41 cm
Читательская аудитория: Postgraduate, research & scholarly
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Описание: Describes the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis. This book presents a set of enzymatic methods for determining DNA breaks.
Дополнительное описание: Формат: 229x152
Илюстрации: 33
Круг читателей: Molecular biologists, cell biologists, pathologists, embryologists, toxicologists, radiobiologists, experimental and clinical pathologists, experimental pharmacologists, research oncologists, drug design specialists, and environmental scientists
Ключевые слова:
Язык: eng


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Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Цена: 11164 р.
Наличие на складе: Есть (3 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

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Two-Hybrid Systems / Methods and Protocols

Автор: MacDonald Paul N.
Название: Two-Hybrid Systems / Methods and Protocols
ISBN: 0896038327 ISBN-13(EAN): 9780896038325
Издательство: Springer
Цена: 11934 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.

Описание: Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast), to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problem of artifacts and false positives and for identifying the interacting partners in important biological systems, including the SMAD and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly.

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In Situ Detection of DNA Damage

Автор: Vladimir V. Didenko (Ed.)
Название: In Situ Detection of DNA Damage
ISBN: 1603274081 ISBN-13(EAN): 9781603274081
Издательство: Springer
Цена: 11164 р.
Наличие на складе: Нет в наличии.

Описание: Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.

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PRINS and In Situ PCR Protocols

Автор: Pellestor Franck
Название: PRINS and In Situ PCR Protocols
ISBN: 1588295494 ISBN-13(EAN): 9781588295491
Издательство: Springer
Цена: 9239 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.

Описание: This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.

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In Situ Hybridization Protocols

Автор: Darby Ian A., Hewitson Tim D.
Название: In Situ Hybridization Protocols
ISBN: 1588294021 ISBN-13(EAN): 9781588294029
Издательство: Springer
Цена: 11934 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.

Описание: This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biologyв„ў series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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In Situ Hybridization in Neurobiology: Advances in Methodology

Автор: Eberwine, James H.;Valentino, Karen L.;Barchas, Ja
Название: In Situ Hybridization in Neurobiology: Advances in Methodology
ISBN: 0195075072 ISBN-13(EAN): 9780195075076
Издательство: Oxford Academ
Цена: 3461 р.
Наличие на складе: Поставка под заказ.

Описание: In situ hybridization has become an important and widely used research tool for neurobiologists. This sequel to the author's monograph "In Situ Hybridization: Applications to Neurobiology" examines recent advances in the technique and its impact on studies of the nervous system.

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In Situ Hybridization: A Practical Approach

Название: In Situ Hybridization: A Practical Approach
ISBN: 0199636583 ISBN-13(EAN): 9780199636587
Издательство: Oxford Academ
Цена: 7450 р.
Наличие на складе: Поставка под заказ.

Описание: This edition contains protocols detailing the major techniques of "in situ" hybridization in the late 1990s. It includes topics such as: "in situ" hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); and analysis using light and electron microscopes.

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Fluorescence in situ hybridization (fish) - application guide

Название: Fluorescence in situ hybridization (fish) - application guide
ISBN: 3540705805 ISBN-13(EAN): 9783540705802
Издательство: Springer
Цена: 11880 р.
Наличие на складе: Нет в наличии.

Описание: Provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), which are used to study many aspects of genomic behavior and alterations. This work describes methods using various cell types and tissues as well as different organisms, such as mammalians.

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Mitochondrial DNA / Methods and Protocols

Автор: Copeland William C.
Название: Mitochondrial DNA / Methods and Protocols
ISBN: 0896039722 ISBN-13(EAN): 9780896039728
Издательство: Springer
Цена: 6541 р.
Наличие на складе: Нет в наличии.

Описание: Internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers.

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DNA Arrays / Methods and Protocols

Автор: Rampal Jang B.
Название: DNA Arrays / Methods and Protocols
ISBN: 089603822X ISBN-13(EAN): 9780896038226
Издательство: Springer
Цена: 9239 р.
Наличие на складе: Есть у поставщика.Поставка под заказ.

Описание: In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct DNA microarrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions, these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project.

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Recombinant Protein Protocols / Detection and Isolation

Автор: Tuan Rocky S.
Название: Recombinant Protein Protocols / Detection and Isolation
ISBN: 0896034003 ISBN-13(EAN): 9780896034006
Издательство: Springer
Цена: 6156 р.
Наличие на складе: Нет в наличии.

Описание: Leading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products-recombinant proteins. These state-of-the-art techniques describe a large number of molecular tags and labels, including enzymes, ligand-binding moieties, and immunodetectable molecules. There are also methods to detect interactive proteins and gene expression-mediated alterations in cellular activity, as well as chapters on in situ detection of gene expression. When combined with a companion volume by the same editor, Recombinant Gene Expression Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.

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PCR Mutation Detection Protocols

Автор: Theophilus Bimal D. M., Rapley Ralph
Название: PCR Mutation Detection Protocols
ISBN: 0896036170 ISBN-13(EAN): 9780896036178
Издательство: Springer
Цена: 5617 р.
Наличие на складе: Нет в наличии.

Описание: Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

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