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Biostatistical Methods, Looney Stephen W.

Biostatistical Methods

Автор: Looney Stephen W.
Название:  Biostatistical Methods   (Стефан Луни: Биостатистические методы)
Издательство: Springer
Классификация:
Клеточная биология

ISBN: 089603951X
ISBN-13(EAN): 9780896039513
ISBN: 0-89603-951-X
ISBN-13(EAN): 978-0-89603-951-3
Обложка/Формат: Hardcover
Страницы: 224
Дата издания: 2002
Серия: Methods in Molecular Biology
Язык: ENG
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes. Major areas of application include microarray analysis, proteomic studies, image quantitation, genetic susceptibility and association, evaluation of new biomarkers, and power analysis and sample size.
Дополнительное описание: Формат: 229x152
Илюстрации: 39
Круг читателей: Molecular biologists working in academia, industry, and government research laboratories
Ключевые слова:
Язык: eng



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Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Цена: 6157 р.
Наличие на складе: Есть (3 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
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Computational systems bioinformatics - methods and biomedical applications

Название: Computational systems bioinformatics - methods and biomedical applications
ISBN: 9812707042 ISBN-13(EAN): 9789812707048
Издательство: World Scientific Publishing
Цена: 9715 р.
Наличие на складе: Есть (1 шт.)
Описание: Computational systems biology is a new and rapidly developing field of research, concerned with understanding the structure and processes of biological systems at the molecular, cellular, tissue, and organ levels through computational modeling as well as novel information theoretic data and image analysis methods. By focusing on either information processing of biological data or on modeling physical and chemical processes of biosystems, and in combination with the recent breakthrough in deciphering the human genome, computational systems biology is guaranteed to play a central role in disease prediction and preventive medicine, gene technology and pharmaceuticals, and other biotechnology fields.This book begins by introducing the basic mathematical, statistical, and data mining principles of computational systems biology, and then presents bioinformatics technology in microarray and sequence analysis step-by-step. Offering an insightful look into the effectiveness of the systems approach in computational biology, it focuses on recurrent themes in bioinformatics, biomedical applications, and future directions for research.
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E. coli Plasmid Vectors / Methods and Applications

Автор: Casali Nicola, Preston Andrew
Название: E. coli Plasmid Vectors / Methods and Applications
ISBN: 1588291510 ISBN-13(EAN): 9781588291516
Издательство: Springer
Цена: 6927 р.
Наличие на складе: Нет в наличии.
Описание: A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
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Gene Mapping, Discovery, and Expression / Methods and Protocols

Автор: Bina Minou
Название: Gene Mapping, Discovery, and Expression / Methods and Protocols
ISBN: 1588295753 ISBN-13(EAN): 9781588295750
Издательство: Springer
Цена: 5926 р.
Наличие на складе: Нет в наличии.
Описание: A collection of cutting-edge computational tools and experimental techniques to study how genes are regulated, and to reconstruct the regulatory networks through which various cell-types are produced. On the computational side, web-based technologies to localize genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential "codes" in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis to characterize the transcription factor binding elements, and methods for isolating and identifying proteins that interact with DNA. The protocols follow the successful Methods in Molecular Biologyв„ў series format, each offering step-by-step instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls.
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Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology)

Автор: Gerdes Svetlana, Osterman Andrei L.
Название: Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology)
ISBN: 1588293785 ISBN-13(EAN): 9781588293787
Издательство: Springer
Цена: 7659 р.
Наличие на складе: Нет в наличии.
Описание: This book contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. All protocols follow the successful Methods in Molecular Biologyв„ў series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.
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Generation of cDNA Libraries / Methods and Protocols

Автор: Ying Shao-Yao
Название: Generation of cDNA Libraries / Methods and Protocols
ISBN: 1588290662 ISBN-13(EAN): 9781588290663
Издательство: Springer
Цена: 6927 р.
Наличие на складе: Нет в наличии.
Описание: Expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation.
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Membrane Transporters / Methods and Protocols

Автор: Yan Qing
Название: Membrane Transporters / Methods and Protocols
ISBN: 1588291049 ISBN-13(EAN): 9781588291042
Издательство: Springer
Цена: 5772 р.
Наличие на складе: Нет в наличии.
Описание: This collection of cutting-edge methodologies for studying membrane transporters and channels takes advantage of all the latest developments in biomedical research, including pharmacogenomcs, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics studies, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microcopy are also described, along with several that may help in the development of novel therapeutics.
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Microarrays / Volume I, Synthesis Methods

Автор: Rampal Jang B.
Название: Microarrays / Volume I, Synthesis Methods
ISBN: 1588295893 ISBN-13(EAN): 9781588295897
Издательство: Springer
Цена: 8082 р.
Наличие на складе: Нет в наличии.
Описание: Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments.Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology.Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.
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G Protein Methods and Protocols

Автор: Mishra Ram K., Baker Glen B., Boulton Alan A.
Название: G Protein Methods and Protocols
ISBN: 0896034909 ISBN-13(EAN): 9780896034907
Издательство: Springer
Цена: 9237 р.
Наличие на складе: Нет в наличии.
Описание: Combining the cutting-edge insights of leading basic and clinical investigators, G Protein Methods and Protocols provides both critical reviews and detailed protocols for studying the medical role played by G proteins and cellular signaling in psychiatric and neurological disease. The mental and neurological disorders covered range from Parkinson's disease and schizophrenia to manic depression, drug addiction, and mood and anxiety disorders.
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Mitochondrial DNA / Methods and Protocols

Автор: Copeland William C.
Название: Mitochondrial DNA / Methods and Protocols
ISBN: 0896039722 ISBN-13(EAN): 9780896039728
Издательство: Springer
Цена: 6542 р.
Наличие на складе: Нет в наличии.
Описание: Internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers.
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Molecular Motors / Methods and Protocols

Автор: Sperry Ann O.
Название: Molecular Motors / Methods and Protocols
ISBN: 1588296652 ISBN-13(EAN): 9781588296658
Издательство: Springer
Цена: 6157 р.
Наличие на складе: Нет в наличии.
Описание: Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.
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Nuclease Methods and Protocols

Автор: Schein Catherine H.
Название: Nuclease Methods and Protocols
ISBN: 0896036790 ISBN-13(EAN): 9780896036796
Издательство: Springer
Цена: 8467 р.
Наличие на складе: Нет в наличии.
Описание: Scientists at the forefront of academic research and pharmaceutical drug development offer their well-polished methodologies for protein nucleases that can be used to determine their activity, structure, interaction with other molecules, and physiological role. Protocols range from methods for characterizing nucleases, to correlating nuclease structure and function, to the use of nucleases in cloning. Recombinant techniques for expressing and isolating nucleases are covered, as well as methods specific for dealing with protein/nucleic acid complexes. Each chapter contains a minireview of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assay in question, and an explanation of the thinking that went into its development. Comprehensive and highly practical, Nuclease Methods and Protocols offers both novice and experienced researchers a deeper understanding of their importance in cell metabolism, growth control mechanisms, and, increasingly, in diagnostics and therapeutics.
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