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Apoptosis Techniques and Protocols, LeBlanc Andréa C.

Apoptosis Techniques and Protocols

: LeBlanc Andréa C.
 Apoptosis Techniques and Protocols   ( )
: Springer
:
:

ISBN: 1588290123
ISBN-13(EAN): 9781588290120
ISBN: 1-58829-012-3
ISBN-13(EAN): 978-1-58829-012-0
/: Hardcover
: 272
: 0.571 .
: 2002
: Neuromethods
: ENG
: 22.35 x 17.53 x 2.36 cm
:
: In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.
: : 229x152
: 53
: Neuroscientists, cell biologists, molecular biologists, endocrinologists, pharmacologists, medicinal chemists, pharmaceutical chemists, pathologists, and geneticists
:
: eng



   
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Recombinant Gene Expression / Reviews and Protocols

: Balbas Paulina, Lorence Argelia
: Recombinant Gene Expression / Reviews and Protocols
ISBN: 1588292622 ISBN-13(EAN): 9781588292629
: Springer
: 10116 .
: .
: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Guide to Research Techniques in Neuroscience,

: Matt Carter
: Guide to Research Techniques in Neuroscience,
ISBN: 0123748496 ISBN-13(EAN): 9780123748492
: Elsevier Science
: 4395 .
: .
: Neuroscience is by definition a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry. A single topic such as the auditory system can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. A young scientist must learn how to read the primary literature and then develop their own experiments. This book offers that scientist an overview of mainstream research techniques, provides guidelines on how to choose one technique over another, offers tips on analyzing data, and provides a list of references for additional detailed study. This book can also assist an experienced scientist understand published studies conducted outside their own subfield. <br><br><p>*Written by Stanford University graduate students in neuroscience to provide a "hands-on" approach for other neuroscience graduate students&nbsp;<br>*Techniques within one field will be compared so that user can select best technique for their experiment<br>*Chapters include references (key articles, books, protocols) for additional detailed study<br>*Data Analysis boxes in each chapter help with data interpretation and&nbsp;offer guidelines on how best to represent results<br>*"Walk-through" boxes guide students through the experiment step-by-step</p>

Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitf

: John T. Corthell
: Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitf
ISBN: 012801461X ISBN-13(EAN): 9780128014615
: Elsevier Science
: 3428 .
: .

Matrix Metalloproteinase Protocols

: Ian M. Clark (Ed.)
: Matrix Metalloproteinase Protocols
ISBN: 1603272984 ISBN-13(EAN): 9781603272988
: Springer
: 10555 .
: (1 .)
: Detailing proven laboratory techniques for the study of MMPs, this book covers the methods including those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances.

Regulatory Protein Modification / Techniques and Protocols

: Hemmings Hugh C.
: Regulatory Protein Modification / Techniques and Protocols
ISBN: 0896034151 ISBN-13(EAN): 9780896034150
: Springer
: 10555 .
: .
: Part I. Introduction. Recombinant Gene Expression Protocols: Overview of Experimental Strategies for the Expression of Recombinant Genes, Rocky S. Tuan. Part II. Expression in E. coli. Designing Expression Plasmid Vectors in E. coli, Paulina Balbás. Design of Bacterial Hosts for lac-Based Expression Vectors, Herbert P. Schweizer and RoxAnn R. Karkhoff-Schweizer. Bacteriophage l-Based Expression Vectors, Alan C. Christensen. Strategies for Gene Fusions, Stefan Ståhl, Per-Åke Nygren, and Mathias Uhlén. Application of the E. coli trp Promoter, Daniel G. Yansura and Steven H. Bass. Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7, George A. Garcia and Shaorong Chong. Expression and Secretion of Proteins in E. coli, Ophry Pines and Masayori Inouye. Synthetic Two-Cistron Expression System, Brigitte E. Schoner. Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translation, Christopher Coleclough. Part III. Expression in Yeast. Yeast Plasmids, Keshav K. Singh and Jack A. Heinemann. Inducible Expression Cassettes in Yeast: GAL4, Lawrence M. Mylin and James E. Hopper. Inducible Expression Cassettes in Yeast: ADH2, Virginia L. Price. Constitutive Expression Vectors: PGK, Ian R. Graham and Alistair Chambers. Part IV. Viral Expression Systems. Design and Construction of Recombinant Vaccinia Viruses, Christopher C. Broder and Patricia L. Earl. A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus, Haifeng Chen and R. Padmanabhan. Design of Retroviral Expression Vectors, Kenine E. Comstock, Nathaniel F. Watson, and John C. Olsen. Use of Defective Herpes-Derived Plasmid Vectors, Filip Lim, Dean Hartley, Philip Starr, Song Song, Phung Lang, Linda Yu, Yaming Wang, and Alfred I. Geller. Part V. Expression in Nonmammalian Higher Eukaryotes. Use of Baculovirus Expression Vectors, David R. O'Reilly. Expression of Exogenous Genes in Xenopus Oocytes, Eggs, and Embryos, Koichiro Shiokawa, Chie Koga, Yuzuru Ito, and Mikihito Shibata. Analysis of Heterologous Gene Expression in Xenopus Blastomeres, Sally A. Moody. Part VI. Expression in Mammalian Cells. Overview of Vector Design for Mammalian Gene Expression, Randal J. Kaufman. Experimental Strategies in Efficient Transfection of Mammalian Cells: Calcium Phosphate and DEAE-Dextran, Gregory S. Pari and Wayne A. Keown. Experimental Strategies in Efficient Transfection of Mammalian Cells: Electroporation, Donald C. Chang. Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer, Rémy A. Aubin, Michael Weinfeld, Marzieh Taghavi, Razmik Mirzayans, and Malcolm C. Paterson. Selection of Transfected Cells: Magnetic Affinity Cell Sorting, Raji Padmanabhan, Snorri S. Thorgeirsson, and R. Padmanabhan. Selection of Transfected Cells and Coamplification of Transfected Genes, Susan E. Kane. Optimization of Growth, Viability, and Specific Productivity for Expression of Recombinant Proteins in Mammalian Cells, Jennie P. Mather, Alison Moore, and Robert Shawley. Part VII. Expression in Transgenic Organisms. Promoters to Express Cloned Genes Uniformly in Drosophila, Jean-Paul Vincent and Charles Girdham. Application of Micromechanical Piercing Structures for Genetic Transformation of Nematodes, Sarwar Hashmi and Randy Gaugler. Strategies in Generating Transgenic Mammals, Olena Jacenko. Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens, Nathalie Allioli, Gérard Verdier, and Catherine Legras. Strategies for the Production of Transgenic Chickens, Robert J. Etches and Ann M. Verrinder Gibbins. Part VIII. Expression in Plants. Plant Cell Transfection by Electroporation, William B. Terzaghi and Anthony R. Cashmore. Transformation and Foreign Gene Expression in Plants Mediated by Microprojectile Bombardment, Henry Daniell. Transformation of the Cereals Using Agrobacterium, Jean Gould. Transformation of Microalgae Using Silicon Carbide Whiskers, Terri G. Dunahay, Sally A. Adler, and Jonathan W. Jarvik. Index.

Genetic Techniques for Biological Research: A Case Study Approach

: Corinne A. Michels
: Genetic Techniques for Biological Research: A Case Study Approach
ISBN: 0471899216 ISBN-13(EAN): 9780471899211
: Wiley
: 5445 .
: .
: Teaches the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology. This work uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis.

Neuropeptide Techniques

: Gozes Illana
: Neuropeptide Techniques
ISBN: 1588294684 ISBN-13(EAN): 9781588294685
: Springer
: 8796 .
: .
: Neuropeptides are very small proteins that are released by nerve cells to influence neighboring cells and tissues.&nbsp; Neuropeptides exhibit multiple functions as hormones and regulators.&nbsp; As such, many techniques are required to understand the contribution and functions of these important messenger molecules in health and disease processes.&nbsp; Research in the neuropeptide field is aimed toward a better understanding of the physiology of the human body and toward the development of better diagnostics and therapeutics.&nbsp; This books contents will familiarize the audience with forefront techniques in the field.

In Vitro Neurochemical Techniques

: Boulton Alan A., Baker Glen B., Bateson Alan N.
: In Vitro Neurochemical Techniques
ISBN: 0896035093 ISBN-13(EAN): 9780896035096
: Springer
: 9236 .
: .

Cell Neurobiology Techniques

: Boulton Alan A., Baker Glen B., Bateson Alan N.
: Cell Neurobiology Techniques
ISBN: 0896035107 ISBN-13(EAN): 9780896035102
: Springer
: 9236 .
: .

Biotechnology: Proteins to PCR / A Course in Strategies and Lab Techniques

: Burden David W., Whitney Donald B.
: Biotechnology: Proteins to PCR / A Course in Strategies and Lab Techniques
ISBN: 0817638431 ISBN-13(EAN): 9780817638436
: Birkhauser Verlag AG
: 9088 .
: .

Techniques in Quantification and Localization of Gene Expression

: Patterson Bruce K.
: Techniques in Quantification and Localization of Gene Expression
ISBN: 0817640347 ISBN-13(EAN): 9780817640347
: Birkhauser Verlag AG
: 13038 .
: .
: The molecular basis of disease is the driving force in biomedical reserach. Gene detection, quantification, and localization are required to determine disease pathogenesis. In as much as DNA is the genetic potential in a particular organism, RNA and protein expression are the functional result of our genetic program. "Techniques in Localization of Gene Expression" describes in great detail the methods used to quantify and localize gene expression. Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. "Techniques" emphasizes optimized methods to ensure full recovery and preservaiton of targets from specimen acquisition through detection of specific targets and final analysis. Situ Analyses of cells and tissues are also discussed. One of the weaknesses in biomedical research has been the use of in vitro model systems consisting of cell lines that may or may not mimic the in vivo conditions. These model systems are necessary to ask several questions on cell s and tissues with controllled variables int he absense of strategies to ask many questions at the same time. Multiparameter analysis can cirmcumvent the necessity fo in vitro model systems by allowing many quesitons to be answered on a cell or tissue at a single point in time. Using fluorescent dyes, on can answer many questions about a particular cell or tissue. One color, one question is an overriding theme of this book. The limitations of this approach are the number of colors in the spectrum and our ability to resolve them. Readers will find working protocols that have been developed and used routinely in the author's laboratory. Representative applications will be described using the various gene quantification and localization protocols to illustrate the utility of the techniques.

Techniques in Molecular Systematics and Evolution

: DeSalle Rob, Giribet Gonzalo, Wheeler Ward
: Techniques in Molecular Systematics and Evolution
ISBN: 376436257X ISBN-13(EAN): 9783764362577
: Birkhauser Verlag AG
: 10951 .
: .
: The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the


 
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