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Affinity Chromatography / Methods and Protocols, Zachariou Michael

Affinity Chromatography / Methods and Protocols

јвтор: Zachariou Michael
Ќазвание:  Affinity Chromatography / Methods and Protocols   (јффинна€ хроматографи€)
»здательство: Springer
 лассификаци€:
ћедицинска€ генетика
‘армакологи€
јналитическа€ хими€
 леточна€ биологи€
Ѕиотехнологи€

ISBN: 1588296598
ISBN-13(EAN): 9781588296597
ISBN: 1-58829-659-8
ISBN-13(EAN): 978-1-58829-659-7
ќбложка/‘ормат: Hardback
—траницы: 360
¬ес: 0.687 кг.
ƒата издани€: 17.12.2007
—ери€: Methods in Molecular Biology
язык: ENG
»здание: 2 rev ed
»ллюстрации: 47 black & white illustrations, 23 black & white tables, 8 black & white halftones, 39 black & white
–азмер: 229 x 152
„итательска€ аудитори€: Postgraduate, research & scholarly
—сылка на »здательство: Link
–ейтинг:
ѕоставл€етс€ из: √ермании
ќписание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
ќписание: Offers the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This book also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers.
ƒополнительное описание: ‘ормат: 229x152
»люстрации: 47
 руг читателей: Geneticists, molecular biologists, biotechnologists, pharmaceutical scientists
 лючевые слова: Separation techiques
Interaction science
Post-genomic era
Purification tags
язык: eng
»здание: 2nd ed.
ќглавление: PROPOSED TABLE OF CONTENTS 1 Affinity Chromatography: History, Perspectives, Limitations and Prospects Ana Cec√≠­lia A. Roque and Christopher R. Lowe VARIOUS MODES OF AFFINITY CHROMATOGRAPHY 2 Immobilized Metal Ion Affinity Chromatography of Native Proteins Adam Charlton and Michael Zachariou 3 Affinity Precipitation of Proteins Using Metal Chelates Ashok Kumar, Igor Yu. Galaev and Bo Mattiasson 4 Immunoaffinity Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 5 Dye-ligand Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 6 Purification of Proteins Using Displacement Chromatography Nihal Tugcu AFFINITY CHROMATOGRAPHY USING PURIFICATION TAGS 7 Rationally Designed Ligands for use in Affinity Chromatography: an Artificial Protein L Ana Cec√≠­lia A. Roque and Christopher Lowe 8 Phage display of Peptides in Ligand Selection for use in Affinity Chromatography Joanne L Casey, Andrew M. Coley and Michael Foley 9 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Gareth M. Forde 10 Immobilized Metal Ion Affinity Chromatography of Histidine-tagged fusion proteins Adam Charlton and Michael Zachariou 11 Methods for the Purification of HQ-Tagged Proteins Beckky Godat, Laurie Engel, Natalie Betz and Tonny M. Johnson 12 Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis-Refolding Methods. Leonard K. Pattenden and Walter G. Thomas 13 Methods for the Detection of Protein-Protein and Protein-DNA Interactions using Halo-TagвДҐMarjeta Urh, Danette Hartzell, Jacqui Mendez and Keith Wood 14 Site-specific Cleavage of Fusion Proteins Adam Charlton 15 The Use of TAGzyme for the efficient Removal of N-terminal His-tags Jos√© Arnau, Conni Lauritzen, Gitte E. Petersen and John Pedersen VARIOUS APPLICATIONS OF AFFINITY CHROMATOGRAPHY. 16 Affinity Processing of Cell-containing Feeds Using Monolithic Macroporous Hydrogels, Cryogels Igor Yu. Galaev and Bo Mattiasson 17 Monolithic Bioreactors for Macromolecules Mojca Ben_ina, Katja Ben_ina, Ale_ Podgornik and Ale_ _trancar 18 Plasmid DNA Purification via the Use of a Dual Affinity Protein Gareth M. Forde 19 Affinity Chromatography of Phosphorylated Proteins Grigoriy S. Tchaga 20 Protein Separation Using Phospholipid Chromatography Tzong-Hsien Lee and Marie-Isabel Aguilar 21 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Neils H.H. Heegaard, Christian Schou and Jesper √Шstergaard



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Ќазвание: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
»здательство: Springer
÷ена: 1092 р.
Ќаличие на складе: ≈сть (2 шт.)
ќписание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
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јвтор: Lundanes
Ќазвание: Chromatography: Basic Principles, Sample Preparations and Related Methods
ISBN: 3527336206 ISBN-13(EAN): 9783527336203
»здательство: Wiley
÷ена: 2860 р.
Ќаличие на складе: ѕоставка под заказ.
ќписание: This book is the perfect introduction into a methodology which is the underlying principle of the vast majority of separation methods worldwide – chromatography. Everyone working in a lab environment must be familiar with the basis of these technolo
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јвтор: R. Andrew Shalliker
Ќазвание: Hyphenated and Alternative Methods of Detection in Chromatography
ISBN: 084939077X ISBN-13(EAN): 9780849390777
»здательство: Taylor&Francis
÷ена: 11264 р.
Ќаличие на складе: ѕоставка под заказ.
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Ќазвание: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
»здательство: Springer
÷ена: 1092 р.
Ќаличие на складе: ≈сть (4 шт.)
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ISBN: 1588295753 ISBN-13(EAN): 9781588295750
»здательство: Springer
÷ена: 5926 р.
Ќаличие на складе: Ќет в наличии.
ќписание: A collection of cutting-edge computational tools and experimental techniques to study how genes are regulated, and to reconstruct the regulatory networks through which various cell-types are produced. On the computational side, web-based technologies to localize genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential "codes" in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis to characterize the transcription factor binding elements, and methods for isolating and identifying proteins that interact with DNA. The protocols follow the successful Methods in Molecular BiologyвДҐ series format, each offering step-by-step instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls.
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Ќазвание: Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology)
ISBN: 1588293785 ISBN-13(EAN): 9781588293787
»здательство: Springer
÷ена: 7659 р.
Ќаличие на складе: Ќет в наличии.
ќписание: This book contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. All protocols follow the successful Methods in Molecular BiologyвДҐ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.
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ISBN: 1588290662 ISBN-13(EAN): 9781588290663
»здательство: Springer
÷ена: 6927 р.
Ќаличие на складе: Ќет в наличии.
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Ќазвание: Epidermal Growth Factor / Methods and Protocols
ISBN: 1588294218 ISBN-13(EAN): 9781588294210
»здательство: Springer
÷ена: 5926 р.
Ќаличие на складе: Ќет в наличии.
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ISBN: 1588292673 ISBN-13(EAN): 9781588292674
»здательство: Springer
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»здательство: Springer
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»здательство: Springer
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