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Cloning Gene Expression, Hardin


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Цена: 11088.00р.
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Автор: Hardin
Название:  Cloning Gene Expression
Перевод названия: Клонирование гена
ISBN: 9780195132946
Издательство: Oxford Academ
Классификация:


ISBN-10: 0195132947
Обложка/Формат: Paperback
Страницы: 444
Вес: 1.01 кг.
Дата издания: May 17, 2001
Язык: English
Иллюстрации: Numerous tables
Размер: 281 x 216 x 20
Читательская аудитория: Postgraduate, research & scholarly
Подзаголовок: Experimental Procedures and Process Rationale
Ссылка на Издательство: Link
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Поставляется из: Англии
Описание: The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyzse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, southern and western blotting and cominatorial techniques are empsized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergraduate students, beginning graduates, and practising professionals engaged in a broad range of pursuits. Each experiment is accompanied by detailed process rationale.
Дополнительное описание: Кол-во стр.: 444
Формат: 278 x 215
Дата издания: 2001
Страна: USA
Круг читателей: undergraduate; postgraduate; research, professional
Иллюстрации: 61 figures, index
Вес: 1012




Heterologous Expression of Membrane Proteins

Автор: Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493936352 ISBN-13(EAN): 9781493936359
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.


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