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Gene Delivery to Mammalian Cells / Volume 2: Viral Gene Transfer Techniques, Heiser William C.



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Автор: Heiser William C.
Название:  Gene Delivery to Mammalian Cells / Volume 2: Viral Gene Transfer Techniques
ISBN: 9781588290953
Издательство: Springer
Классификация:
ISBN-10: 1588290956
Обложка/Формат: Hardback
Страницы: 592
Вес: 1.142 кг.
Дата издания: 2004
Серия: Methods in Molecular Biology
Язык: English
Иллюстрации: Biography
Размер: 23.60 x 16.28 x 3.07
Читательская аудитория: Postgraduate, research & scholarly
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Дополнительное описание: Формат: 229x152
Илюстрации: 69
Круг читателей: Virologists, biotechnologists, molecular biologists, cell biologists, and geneticists working in industry and academia
Ключевые слова:
Язык: eng





DNA Cloning : Vol 4. Mammalian Systems: A Practical Approach

Автор: Hames, B.D.
Название: DNA Cloning : Vol 4. Mammalian Systems: A Practical Approach
ISBN: 0199634807 ISBN-13(EAN): 9780199634804
Издательство: Oxford Academ
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Цена: 11641 р.
Наличие на складе: Поставка под заказ.

Описание: This volume describes techniques for the genetic manipulation of mammalian cells, including gene amplification, retroviral and adenoviral vectors, and the production of transgenic animals.

Non-viral Gene Therapy / Gene Design and Delivery

Автор: Taira Kazunari, Kataoka Kazunori, Niidome Takuro
Название: Non-viral Gene Therapy / Gene Design and Delivery
ISBN: 4431251227 ISBN-13(EAN): 9784431251224
Издательство: Springer
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Цена: 18479 р.
Наличие на складе: Поставка под заказ.

Описание: The development of gene medicine based on the concept of molecular therapy has opened new medical horizons. Among the many techniques in gene medicine, gene design and delivery are especially significant in clinical applications. This book presents state-of-the-art information on non-viral gene-delivery techniques, covering a broad spectrum of disciplines that include chemistry, molecular biology, cell biology, and pharmacokinetics. Major sections introduce molecules for gene delivery and their properties; technologies of controlled gene delivery in vitro and in vivo; therapeutic genes and the status of clinical applications; and the design of genes based on current RNA technology, with revolutionary developments in the world of RNAi shown to be key factors in gene medicine. This groundbreaking work is an invaluable resource for researchers and engineers in genetic engineering, molecular medicine, biochemical engineering, and biotechnology.

Gene Delivery to Mammalian Cells / Volume 1: Nonviral Gene Transfer Techniques

Автор: Heiser William C.
Название: Gene Delivery to Mammalian Cells / Volume 1: Nonviral Gene Transfer Techniques
ISBN: 1588290867 ISBN-13(EAN): 9781588290861
Издательство: Springer
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Цена: 18479 р.
Наличие на складе: Поставка под заказ.

Описание: Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Mammalian and Avian Transgenesis - New Approaches

Автор: Pease Shirley, Lois Carlos
Название: Mammalian and Avian Transgenesis - New Approaches
ISBN: 354028415X ISBN-13(EAN): 9783540284154
Издательство: Springer
Цена: 31070 р.
Наличие на складе: Поставка под заказ.

Описание: Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Mammalian Cell Viability

Автор: Stoddart
Название: Mammalian Cell Viability
ISBN: 1617791075 ISBN-13(EAN): 9781617791079
Издательство: Springer
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Цена: 13859 р.
Наличие на складе: Поставка под заказ.

Описание: Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Viral Pathogenesis and Autoimmunity.2002

Автор: Nathanson
Название: Viral Pathogenesis and Autoimmunity.2002
ISBN: 0781711037 ISBN-13(EAN): 9780781711036
Издательство: Lippincott Williams & Wilkins
Цена: 6600 р.
Наличие на складе: Поставка под заказ.

Описание: Written by eminent authorities on viral pathogenesis and immunity, this text provides a coherent, concise introduction to the field. The authors focus on fundamental principles and present a well-organized overview that is easy for readers to absorb.Section 1, essentials of viral pathogenesis , describes the sequential events in viral infection, the localization of viral infections, and the variety of virus-cell interactions.Section 2, host responses to viral infection , examines immune responses, including virus-induced immunopathology and immunosuppression.Section 3, virus-host interactions , covers virulence, viral persistence, virus-induced oncogenesis, and host susceptibility.Section 4, prevention of viral infections , explains the key principles of vaccine development.

Viral Vectors for Treating Diseases of the Nervous System,55

Автор: David S. Latchman
Название: Viral Vectors for Treating Diseases of the Nervous System,55
ISBN: 0123668565 ISBN-13(EAN): 9780123668561
Издательство: Elsevier Science
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Цена: 20790 р.
Наличие на складе: Поставка под заказ.

Описание: Gene therapy offers potential for the treatment of various incurable diseases of the nervous system. This volume describes a number of viral vectors developed for achieving high efficiency gene delivery to the brain. Vectors described include those based on adenovirus, adeno-associated virus, Herpes Simplex Virus, and other retroviruses.

Cell Death in Mammalian Ovary

Автор: V?zquez-Nin
Название: Cell Death in Mammalian Ovary
ISBN: 9400711336 ISBN-13(EAN): 9789400711334
Издательство: Springer
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Цена: 18349 р.
Наличие на складе: Поставка под заказ.

Описание: The ovary is a suitable organ for studying the processes of cell death. Cell death was first described in the rabbit ovary (Graaffian follicles), the phenomenon being called ‘chromatolysis’. To date, it is recognized that various forms of cell death (programmed cell death, apoptosis and autophagy) are essential components of ovarian development and function. Programmed cell death is responsable for the ovarian endowment of primordial follicles around birth; in the prepuberal and adult period, apoptosis is a basic mechanism by which oocytes are eliminated by cancer therapies and environmental toxicants; in the ovarian cycle, follicular atresia and luteal regression involve follicular cell apoptosis. Finally, abnormalities in cell death processes may lead to ovarian disease such as cancer and chemoresistance. In this book, after an introductory description of various forms of cell death and of the ovary development and function in mammals, the processes of cell death in ovarian somatic cells and oocytes are described at cytological, physiological and molecular levels and analyzed in the embryonic, prepuberal and adult ovary. A complex array of molecular pathways triggered by extrinsic and intrinsic signals able tor induce or suppress cell death in the same cell, according to cell type and ovary developmental stage, emerges. Physiological interactions with the axis hypothalamus-hypophysis as well as ovarian internal functional signal are also critically reviewed to explain the abortive development of follicles before the beginning of the ovarian cycle. The book conveys information useful to the updating of biologists and physicians who are interested to the ovary biology and functions. Hopefully it should provide also clues for stimulating novel experiments in the study of cell death in the mammalian ovary still at an early stage.

Viral Pathogenesis and Immunity

Автор: Michael Katze
Название: Viral Pathogenesis and Immunity
ISBN: 0128009640 ISBN-13(EAN): 9780128009642
Издательство: Elsevier Science
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Цена: 8310 р.
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Описание: Viral Pathogenesis: From Basics to Systems Biology, Third Edition, has been thoroughly updated to cover topical advances in the evolving field of viral pathogenesis, while also providing the requisite classic foundational information for which it is recognized. . The book provides key coverage of the newfound ability to profile molecular events on a system-wide scale, which has led to a deeper understanding of virus-host interactions, host signaling and molecular-interaction networks, and the role of host genetics in determining disease outcome. . In addition, the content has been augmented with short chapters on seminal breakthroughs and profiles of their progenitors, as well as short commentaries on important or controversial issues in the field. Thus, the reader will be given a view of virology research with perspectives on issues such as biomedical ethics, public health policy, and human health. In summary, the third edition will give the student a sense of the exciting new perspectives on viral pathogenesis that have been provided by recent developments in genomics, computation, modeling, and systems biology.

Viral Transport in Plants

Автор: Waigmann Elisabeth, Heinlein Manfred
Название: Viral Transport in Plants
ISBN: 3540698426 ISBN-13(EAN): 9783540698425
Издательство: Springer
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Цена: 19428 р.
Наличие на складе: Поставка под заказ.

Описание: Intercellular communication in plants plays pivotal roles in coordination and control of development and defence responses and involves the trafficking of RNA and protein macromolecules through cytoplasmic cell wall channels termed plasmodesmata. Viruses pirate this existing macromolecular transport pathway to spread infection and, therefore, represent important tools to investigate the cellular mechanisms that govern intercellular communication. The book provides a state of the art overview of the intricate functional virus:host relationships that allow a virus or viroid to move cell-to-cell and systemically through the plant, as well as from plant to plant, and, thus, to spread infection. The book also illustrates the mechanisms by which viruses overcome plant defence responses, such as RNA silencing. Arabidopsis is introduced as a plant host eminently suitable for genetic approaches to identify novel players in plant:virus interactions.

Nuclear Export of Viral RNAs

Автор: Hauber J., Vogt P.K.
Название: Nuclear Export of Viral RNAs
ISBN: 3540412786 ISBN-13(EAN): 9783540412786
Издательство: Springer
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Цена: 16746 р.
Наличие на складе: Поставка под заказ.

Описание: This volume of CTMI provides a timely overview to the mechanisms and factors that mediate nuclear export of viral RNAs. It also covers the structure of nuclear pore complexes and experimental approaches to investigate nuclear export.


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