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Embryonic Stem Cell Protocols / Volume I: Isolation and Characterization, Turksen Kursad



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Автор: Turksen Kursad
Название:  Embryonic Stem Cell Protocols / Volume I: Isolation and Characterization
ISBN: 9781588294982
Издательство: Springer
Классификация:
ISBN-10: 1588294986
Обложка/Формат: Hardback
Страницы: 536
Вес: 0.953 кг.
Дата издания: 15.02.2006
Серия: Methods in Molecular Biology
Язык: English
Издание: 2nd ed. 2006
Иллюстрации: 216 black & white illustrations, 90 black & white
Размер: 23.57 x 16.15 x 2.97
Читательская аудитория: Professional & vocational
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Дополнительное описание: Формат: 229x152
Илюстрации: 216
Круг читателей: Developmental biologists, stem cell biologists, cell biologists, molecular biologists, clinician scientists working in regenerative medicine, and oncologists with stem cell interests
Ключевые слова:
Язык: eng
Издание: 2nd ed.





      Старое издание
Embryonic Stem Cells

Автор: Turksen
Название: Embryonic Stem Cells
ISBN: 0896038815 ISBN-13(EAN): 9780896038813
Издательство: Wiley
Цена: 13406 р.
Наличие на складе: Поставка под заказ.
Описание: Embryonic stem cells are one of the most discussed biomedical issues of the day as they have generated new possibilities for increasing our understanding of cell lineages and differentiation. ES cells are providing a limitless source of specific cell populations, and this book provides the detailed methods for use of ES cells to study various lineages and tissue types. The first part of the volume includes detailed protocols for ES cell isolation, maintenance, modulation of gene expression, and studies of ES cell cycle and apoptosis. It also provides the necessary protocols to generat diverse cell and tissue


Human Embryonic Stem Cell Protocols

Автор: Turksen
Название: Human Embryonic Stem Cell Protocols
ISBN: 1607613689 ISBN-13(EAN): 9781607613688
Издательство: Springer
Рейтинг:
Цена: 17901 р.
Наличие на складе: Есть (1 шт.)
Описание: Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells.

Adult and Embryonic Stem Cells

Автор: Kursad Turksen
Название: Adult and Embryonic Stem Cells
ISBN: 162703952X ISBN-13(EAN): 9781627039529
Издательство: Springer
Рейтинг:
Цена: 19428 р.
Наличие на складе: Поставка под заказ.

Описание: Collecting reviews first presented at the 2010 Stem Cell Symposia in Samsun, Turkey, this book covers embryonic and adult stem cell sources. Reviews the current state of knowledge as well as the challenges that underlie translational medicine applications.

RNA Isolation and Characterization Protocols

Автор: Rapley Ralph, Manning David L.
Название: RNA Isolation and Characterization Protocols
ISBN: 0896033937 ISBN-13(EAN): 9780896033931
Издательство: Springer
Рейтинг:
Цена: 9234 р.
Наличие на складе: Поставка под заказ.

Описание: Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols is the single best collection of easily reproducible, step-by-step RNA techniques now available for today's bench scientists.

RNA Isolation and Characterization Protocols

Автор: Rapley Ralph, Manning David L.
Название: RNA Isolation and Characterization Protocols
ISBN: 0896034941 ISBN-13(EAN): 9780896034945
Издательство: Springer
Рейтинг:
Цена: 21587 р.
Наличие на складе: Поставка под заказ.

Описание: Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols is the single best collection of easily reproducible, step-by-step RNA techniques now available for today's bench scientists.

Gene Isolation and Mapping Protocols

Автор: Boultwood Jacqueline
Название: Gene Isolation and Mapping Protocols
ISBN: 0896033821 ISBN-13(EAN): 9780896033825
Издательство: Springer
Рейтинг:
Цена: 16746 р.
Наличие на складе: Поставка под заказ.

Описание: An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Biomembrane Protocols / I. Isolation and Analysis

Автор: Graham John M., Higgins Joan A.
Название: Biomembrane Protocols / I. Isolation and Analysis
ISBN: 0896032361 ISBN-13(EAN): 9780896032361
Издательство: Springer
Рейтинг:
Цена: 16746 р.
Наличие на складе: Поставка под заказ.

Описание: Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the isolation and compositional analysis of membranes and address animal, plant, and microbial cells. The topics covered in this first volume include the isolation of subcellular fractions, centrifugation, the extraction and quantitation of lipids, the separation and analysis of proteins, and the isolation and characterization of glycosylated molecules, glycoproteins, proteoglycans, and glycolipids.

Recombinant Protein Protocols / Detection and Isolation

Автор: Tuan Rocky S.
Название: Recombinant Protein Protocols / Detection and Isolation
ISBN: 089603481X ISBN-13(EAN): 9780896034815
Издательство: Springer
Рейтинг:
Цена: 21587 р.
Наличие на складе: Поставка под заказ.

Описание: Leading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products-recombinant proteins. These state-of-the-art techniques describe a large number of molecular tags and labels, including enzymes, ligand-binding moieties, and immunodetectable molecules. There are also methods to detect interactive proteins and gene expression-mediated alterations in cellular activity, as well as chapters on in situ detection of gene expression. When combined with a companion volume by the same editor, Recombinant Gene Expression Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.

Pancreatic Islet Isolation

Автор: Ram?rez-Dom?nguez
Название: Pancreatic Islet Isolation
ISBN: 3319398229 ISBN-13(EAN): 9783319398228
Издательство: Springer
Рейтинг:
Цена: 18349 р.
Наличие на складе: Поставка под заказ.

Описание:

Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, access to high quality preparations of pancreatic islets is fundamental for in vitro studies and to test pre-clinical applications in animal models in vivo. Access to healthy human islets is also crucial to improve transplantation procedures for diabetes. Given the susceptibility of pancreatic islets to the enzymatic digestion and mechanical stress required to obtain them, the isolation of islets is often considered as the delicate “work of a craftsman”. This book, which is aimed at beginners and experts alike, is a survey of the current state-of–the-art in this field and it centres on the challenges, pitfalls and peculiarities of pancreatic islet isolation in the different species used in pre-clinical and clinical applications. It explores the similarities and differences between human islets and those from other relevant species (rodents, pigs and non-human primates), and how these influence islet isolation. The ultimate goal of this book is to improve the outcome of islet isolation and transplantation in pre-clinical and clinical applications.
Peptide Characterization and Application Protocols

Автор: Fields Gregg B.
Название: Peptide Characterization and Application Protocols
ISBN: 1588295508 ISBN-13(EAN): 9781588295507
Издательство: Springer
Рейтинг:
Цена: 17901 р.
Наличие на складе: Поставка под заказ.

Описание: Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.

Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

Автор: Nieden
Название: Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases
ISBN: 160761961X ISBN-13(EAN): 9781607619611
Издательство: Springer
Рейтинг:
Цена: 16746 р.
Наличие на складе: Поставка под заказ.

Описание: Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.

Gene Isolation and Mapping Protocols

Автор: Boultwood Jacqueline
Название: Gene Isolation and Mapping Protocols
ISBN: 0896034828 ISBN-13(EAN): 9780896034822
Издательство: Springer
Рейтинг:
Цена: 19056 р.
Наличие на складе: Поставка под заказ.

Описание: An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Human Embryonic Stem Cells Handbook

Автор: Turksen
Название: Human Embryonic Stem Cells Handbook
ISBN: 1617797936 ISBN-13(EAN): 9781617797934
Издательство: Springer
Рейтинг:
Цена: 16746 р.
Наличие на складе: Поставка под заказ.

Описание: Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult.  In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology.  Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.


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