RNA Silencing / Methods and Protocols, Carmichael Gordon
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 11780 р. 16829.00-30% Наличие на складе: Есть (3 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Описание: Selective cytoplasmic organelle and protein targeting has long been thought to constitute the sole determinant of cell polarity and complexity. This view has been changed, however, by the discovery of differential subcellular RNA distribution patterns. Over the past decade it has become clear that selective mRNA sorting and translation contributes to an accumulation of cognate proteins at discrete cellular sites. In this book, various systems are discussed (e.g. Xenopus oocytes, mammalian brain, invertebrate nervous system) in terms of RNA trafficking, RNA-targeting sequences (cis-elements) and RNA-transporting proteins (trans-factors), activity-dependent translational regulation and the significance of the cytoskeleton for neuronal function and plasticity. It also discusses nucleocytoplasmic export of mRNA and viral RNA as another example of subcellular RNA kinesis.
Описание: This hand-on guide to RNA interference makes the power of targeted gene silencing available to any laboratory with a basic equipment for the handling of nucleic acids. In easy to follow step-by-step protocols you will learn how RNAi works in plants, flies and mammals how to design the most efficient RNAi constructs how to achieve transient, stable and conditional RNAi in cell cultures how to determine the efficiency of an RNAi experiment how to use RNAi for gene therapy All protocols have been thoroughly tested in the author's laboratory. Examples of successful experiments and troubleshooting hints are provided to help in establishing your own successful RNAi experiments. Includes a list of suppliers for RNAi reagents and equipment and a glossary of terms.
Автор: Galun Esra Название: Rna silencing ISBN: 9812562060 ISBN-13(EAN): 9789812562067 Издательство: World Scientific Publishing Рейтинг: Цена: 13357 р. Наличие на складе: Поставка под заказ.
Описание: This book handles an emerging, central issue in molecular genetics and the development of eukaryotes: the control of gene expression by small species of RNA. As an exciting new field of endeavor, it is the first book by a single author to deal comprehensively with RNA silencing. The book provides the historical background of the field preceding the seminal work by Fire and associates in 1998 on the impact of small double-stranded RNA on the expression of nematode genes, which is considered the beginning of RNA silencing research. RNA silencing is described in a wide range of plants and animals including protozoa, simple metazoa, insects, non-mammalian vertebrates, and mammals. In each case the experimental results are provideith the accompanying background and with illustrations. There is also an appendix on the prospective use of RNA silencing in gene therapy, which is intended as a guide for investigators wishing to explore this possibility.
Автор: Mouldy Sioud (Ed.) Название: siRNA and miRNA Gene Silencing ISBN: 1603275460 ISBN-13(EAN): 9781603275460 Издательство: Springer Рейтинг: Цена: 15894 р. Наличие на складе: Поставка под заказ.
Описание: Explores the advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA`s unwanted effects and promote successful use in patients. This title is suitable for those with an interest in RNAi, gene regulation, and various therapies.
Описание: The potential for the development of therapeutic oligonucleotides into clinical medicines and their use as basic research tools are explored in this volume,
which is the proceedings of the 7th NIH Symposium on Therapeutic Oligonucleotides. The focus is on antisense, RNAi, triple-helix, gene repair, DNA chips, and CpG immune
modulatory oligonucleotides. Specific chapters address designing better siRNAs, splice switching oligonucleotides, selective delivery of oligonucl
otides, and medicinal drugs by receptor-mediated endocytosis, development of a function overriding siRNA silencing in mammalian cells, transcription factor decoys, and modified
oligonucleotide hybridization and genetic insertion.
Note: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal
subscriptions. Academy Members: Please contact the New York Academy of Sciences directly to place your order.
Members of the New York Academy of Science receive
full-text access to the Annals online and discounts on print volumes.
Описание: Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
Описание: Explores the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. This book includes brief introductions to the material, lists of necessary materials and reagents, step-by-step, and readily reproducible laboratory protocols.
Автор: Elliott Michael Название: Molecular Biology of RNA ISBN: 0199288372 ISBN-13(EAN): 9780199288373 Издательство: Oxford Academ Рейтинг: Цена: 4266 р. Наличие на складе: Поставка под заказ.
Описание: Molecular Biology of RNA provides a broad survey of RNA structure and function. Describing the life cycle of an RNA, it reveals how hugely diverse gene products stem from a relatively modest gene pool. It also describes how RNA molecules have a range of biological functions beyond messenger RNA, including catalysts and regulatory molecules.
Название: Rna interference ISBN: 3540751564 ISBN-13(EAN): 9783540751564 Издательство: Springer Рейтинг: Цена: 19224 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In the last few years the major effect that RNAi has had in invertebrate systems is beginning to take hold in mammalian systems through both single gene knockdown experiments and genome-scale screens. Through the introduction of RNAi, mammalian systems have finally gained admittance to the pantheon of model genetic systems.
Автор: Jensen Название: RNA Exosome ISBN: 1441978402 ISBN-13(EAN): 9781441978400 Издательство: Springer Рейтинг: Цена: 14959 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Assembling work by prominent researchers in the field of RNA exosomes, this excellent introduction to the fascinating protein complex provides a comprehensive account of the proteins` many functions as well as a historical account of the field`s development.
Название: RNA Editing ISBN: 0199638152 ISBN-13(EAN): 9780199638154 Издательство: Oxford Academ Рейтинг: Цена: 15092 р. Наличие на складе: Поставка под заказ.
Описание: This analysis of "RNA editing" looks at the major types of this form of RNA processing. The book explains the fundamental principles as well as detailing advances in the field. Examples of edited RNAs are provided but the text highlights the mechanistic diversity of the various types.
ООО "Логосфера " Тел:+7(495) 980-12-10 www.logobook.ru