Описание: In Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols, a panel of world authorities present cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, X-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47 phox translocation, and microsphere delivery systems. Two companion volumes, Oxidative Stress Biomarkers and Antioxidant Protocols (2002), and Free Radical and Antioxidant Protocols (1998), focus on the related biochemical and cellular biological methods.
Описание: Oxidative Stress and Antioxidant Protection: The Science of Free Radical Biology and< Disease provides an overview of the basic principles of free radical formation. The text delves into free radical formation in molecular biology and its effect on sub
Автор: Armstrong Donald Название: Free Radical and Antioxidant Protocols ISBN: 0896034720 ISBN-13(EAN): 9780896034723 Издательство: Springer Рейтинг: Цена: 8355 р. Наличие на складе: Поставка под заказ.
Описание: This volume presents an outstanding collection of state-of-the-art methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation and technology-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. The laboratory-tested free-radical assays described here in detail will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.
Описание: Focusing on the molecular and chemical bases of oxidative stress, which can cause cancer as well as cardiovascular and neurodegenerative diseases, Molecular Basis of Oxidate Stress discusses the role of free radicals in disease, diagnosis, and therapeutics.
The present work evaluates the toxic effects of some environmental stressors on fish eggs and larvae and describes the biomarker responses of fish from locations with varying levels of pollution. Development of the main groups of biomarkers is discussed. The book demonstrates general trends and specific peculiarities of biomarker induction in early fish life and their association with the animal's developmental stages. It addresses responses of fish eggs and larvae to pollution under experimental conditions and presents information obtained from in situ studies. Chapters describe xenobiotics accumulation, anoxia and hypoxia, increasing temperature, eutrophication, and other unfavorable environmental factors, including biotic and abiotic factors, and their impact on fish embryos. They also examine fluctuations in biomarker levels in fish eggs and larvae that have been impacted by climate changes and discuss possible scenarios, especially for fish population size, reproduction, growth, development and biodiversity.
Описание: The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well. Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome Explains the hows and whys of using mass spectrometry in proteomic analysis Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis Comments on future challenges and how they may be addressed Includes sections on troubleshooting
Автор: Bianchi, Thomas S. Canuel, Elizabeth A. Название: Chemical biomarkers in aquatic ecosystems ISBN: 0691134146 ISBN-13(EAN): 9780691134147 Издательство: Wiley Рейтинг: Цена: 12012 р. Наличие на складе: Поставка под заказ.
Описание: Looks at the application of chemical biomarkers to aquatic ecosystems. This book describes the structure, biochemical synthesis, analysis, and reactivity of each class of biomarkers. It offers a selection of relevant applications to aquatic systems, including lakes, rivers, estuaries, oceans, and paleoenvironments.
Автор: Toshinori Hoshi; Robert Pignolo; Stefan H. Heinema Название: Oxidative Stress and Aging: ISBN: 1603275487 ISBN-13(EAN): 9781603275484 Издательство: Springer Рейтинг: Цена: 10863 р. Наличие на складе: Поставка под заказ.
Описание: Oxidative stress and aging is a multi-dimensional/factorial subject matter encompassing different degrees of organism complexity (from single-cell animals to humans), different molecules (DNA, RNAs, proteins and lipids), different cellular components (mitochondria, cytoplasm and plasma membrane), different tissues and organs, and different human subpopulations including those with different "lifestyles." This book roughly follows this complexity hierarchy, from molecules to organisms; and within each section, pro- and anti-oxidant components will be presented and human implications will be discussed. Some overlap in coverage of topics is anticipated and this is deemed both necessary and preferable for the existing literature is often controversial. The book concludes with a chapter focusing on possible lifespan extension strategies based on our understanding of oxidative stress protection. A short preface in each of the major sections will be included to summarize the section and briefly mention other topics not specifically covered by the chapters within. The following chapter titles are tentative and will be adjusted according the contributors preferences.
Описание: Oxidative stress is the result of an imbalance in pro-oxidant/antioxidant homeostasis that leads to the generation of toxic reactive oxygen species. Brain cells are continuously exposed to reactive oxygen species generated by oxidative metabolism, and in certain pathological conditions defense mechanisms against oxygen radicals may be weakened and/or overwhelmed. DNA is a potential target for oxidative damage, and genomic damage can contribute to neuropathogenesis. It is important therefore to identify tools for the quantitative analysis of DNA damage in models on neurological disorders. This book presents detailed information on various neurodegenerative disorders and their connection with oxidative stress. This information will provide clinicians with directions to treat these disorders with appropriate therapy and is also of vital importance for the drug industries for the design of new drugs for treatment of degenerative disorders.
Автор: Sigrid C. Veasey (Ed.) Название: Oxidative Neural Injury ISBN: 1603273417 ISBN-13(EAN): 9781603273411 Издательство: Springer Рейтинг: Цена: 20900 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Examines evidence and research supporting the importance of oxidative injury in selected animal models of both neurodegenerative diseases and neural injury. This book discusses oxidative injury in a selected model, analyzing the major cellular mechanisms that underpin the progression of neural injury.
Автор: Forman Название: Oxidative Stress and Signal Transduction ISBN: 0412076810 ISBN-13(EAN): 9780412076817 Издательство: Springer Рейтинг: Цена: 21484 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: There is evidence that reactive oxygen species (ROS) can alter cell function by acting upon the intermediates, or second messengers, in signal transductions. Such effects on signaling mechanisms probably account for the role of oxidative stress in inflammation, aging, and cancer. This volume covers oxidative stress and signal transduction.
Автор: Evans Mark D. , Cooke Marcus S. Название: Oxidative Damage to Nucleic Acids ISBN: 0387729739 ISBN-13(EAN): 9780387729732 Издательство: Springer Цена: 22886 р. Наличие на складе: Поставка под заказ.
Описание: This book provides an up-to-date coverage of selected topics in the area of nucleic acid oxidation. The topics have been selected to cover everything from basic chemical mechanisms, repair of damage and the biological and pathological meaning of DNA oxidation. The chapters are authored by leading, research active, international experts in the respective topics. In addition to some of the traditionally covered topics, we have included some areas that receive less attention in similar volumes, for example chapters focusing on damage to nucleic acids by halogenating and nitrating species, gene-specific damage, telomere shortening and damage to promoter regions. Through this approach, we recognize additionally the importance of nucleic acid damage beyond mutation and in pathological conditions other than carcinogenesis. The primary audience for the book would be research scientists and advanced postgraduate students, the book may also be of interest to clinicians with strong research interests. We would expect readers to come away from this book with a greater appreciation of the topic, particularly appraising the reader of areas that may not necessarily have been covered in such a volume before.
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