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Affinity Chromatography / Methods and Protocols, Zachariou Michael

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÷ена: 6196р.   25155р.
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јвтор: Zachariou Michael
Ќазвание:  Affinity Chromatography / Methods and Protocols
ѕеревод названи€: јффинна€ хроматографи€
ISBN: 9781588296597
»здательство: Springer
ISBN-10: 1588296598
ќбложка/‘ормат: Hardback
—траницы: 360
¬ес: 0.692 кг.
ƒата издани€: 17.12.2007
—ери€: Methods in Molecular Biology
язык: English
»здание: 2nd ed. 2008
»ллюстрации: 47 black & white illustrations, 23 black & white tables, 8 black & white halftones, 39 black & white
–азмер: 234 x 160 x 27
„итательска€ аудитори€: Postgraduate, research & scholarly
ѕодзаголовок: Methods and protocols
—сылка на »здательство: Link
ѕоставл€етс€ из: √ермании
ќписание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
ƒополнительное описание: ‘ормат: 229x152
»люстрации: 47
 руг читателей: Geneticists, molecular biologists, biotechnologists, pharmaceutical scientists
 лючевые слова: Separation techiques
Interaction science
Post-genomic era
Purification tags
язык: eng
»здание: 2nd ed.
ќглавление: PROPOSED TABLE OF CONTENTS 1 Affinity Chromatography: History, Perspectives, Limitations and Prospects Ana Cec√≠­lia A. Roque and Christopher R. Lowe VARIOUS MODES OF AFFINITY CHROMATOGRAPHY 2 Immobilized Metal Ion Affinity Chromatography of Native Proteins Adam Charlton and Michael Zachariou 3 Affinity Precipitation of Proteins Using Metal Chelates Ashok Kumar, Igor Yu. Galaev and Bo Mattiasson 4 Immunoaffinity Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 5 Dye-ligand Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 6 Purification of Proteins Using Displacement Chromatography Nihal Tugcu AFFINITY CHROMATOGRAPHY USING PURIFICATION TAGS 7 Rationally Designed Ligands for use in Affinity Chromatography: an Artificial Protein L Ana Cec√≠­lia A. Roque and Christopher Lowe 8 Phage display of Peptides in Ligand Selection for use in Affinity Chromatography Joanne L Casey, Andrew M. Coley and Michael Foley 9 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Gareth M. Forde 10 Immobilized Metal Ion Affinity Chromatography of Histidine-tagged fusion proteins Adam Charlton and Michael Zachariou 11 Methods for the Purification of HQ-Tagged Proteins Beckky Godat, Laurie Engel, Natalie Betz and Tonny M. Johnson 12 Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis-Refolding Methods. Leonard K. Pattenden and Walter G. Thomas 13 Methods for the Detection of Protein-Protein and Protein-DNA Interactions using Halo-TagвДҐMarjeta Urh, Danette Hartzell, Jacqui Mendez and Keith Wood 14 Site-specific Cleavage of Fusion Proteins Adam Charlton 15 The Use of TAGzyme for the efficient Removal of N-terminal His-tags Jos√© Arnau, Conni Lauritzen, Gitte E. Petersen and John Pedersen VARIOUS APPLICATIONS OF AFFINITY CHROMATOGRAPHY. 16 Affinity Processing of Cell-containing Feeds Using Monolithic Macroporous Hydrogels, Cryogels Igor Yu. Galaev and Bo Mattiasson 17 Monolithic Bioreactors for Macromolecules Mojca Ben_ina, Katja Ben_ina, Ale_ Podgornik and Ale_ _trancar 18 Plasmid DNA Purification via the Use of a Dual Affinity Protein Gareth M. Forde 19 Affinity Chromatography of Phosphorylated Proteins Grigoriy S. Tchaga 20 Protein Separation Using Phospholipid Chromatography Tzong-Hsien Lee and Marie-Isabel Aguilar 21 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Neils H.H. Heegaard, Christian Schou and Jesper √Шstergaard

Forced-Flow Layer Chromatography

јвтор: Erno Tyihak
Ќазвание: Forced-Flow Layer Chromatography
ISBN: 012420161X ISBN-13(EAN): 9780124201613
»здательство: Elsevier Science
÷ена: 21916 р.
Ќаличие на складе: ≈сть у поставщика ѕоставка под заказ.


"Forced-Flow Layer Chromatography" takes a close look at the specifics of forced-flow layer chromatography techniques, from their evolution to the nuances of using these techniques in a variety of applications where traditional thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) are not as effective.

This book presents a number of variations of TLC techniques, with special emphasis on the overpressured-layer chromatography (OPLC) technique and newer developments such as the BioArena System for biomedical analysis. The versatility of these forced-flow techniques opens up new avenues for the analysis of a large number of samples for high-throughput screening and for the analysis of very complex matrices, while the development of BioArena extends the use of these techniques to challenging new areas of bioanalysis.
Details a variety of forced-flow techniques, explaining how they markedly reduce developing time and result in less lateral diffusion and more compact spotsEmphasizes the benefits of OPLC separation techniques, a method pioneered by the authors nearly forty years agoDiscusses new developments, such as the BioArena system used to facilitate detection, isolation, and identification of new antimicrobials, antineoplastics, biopesticides, and other biologically active substances

Chiral Separations by Chromatography

јвтор: Ahuja, Satinder
Ќазвание: Chiral Separations by Chromatography
ISBN: 0841236313 ISBN-13(EAN): 9780841236318
»здательство: Oxford Academ
÷ена: 9029 р.
Ќаличие на складе: ѕоставка под заказ.

ќписание: The primary focus of this book is on developing or selecting suitable chromatographic methods for chiral separations. A large number of examples are given to assist in this process, including thin-layer, gas, HPLC, supercritical fluid, capillary electrophoresis, and membrane separations. It
provides information on stereochemistry, TLC, GLC, and CE separation methods and detection; explores the importance of preparative separations; and illustrates the stereoisomeric composition of chemical compounds of pharmaceutical significance. There is discussion of regulations and requirements as
they apply to the development of pharmaceutical compounds, and a detailed section on HPLC methods.


јвтор: Vitha Mark F.
Ќазвание: Chromatography
ISBN: 111927088X ISBN-13(EAN): 9781119270881
»здательство: Wiley
÷ена: 13614 р.
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ќписание: Provides students and practitioners with a solid grounding in the theory of chromatography, important considerations in its application, and modern instrumentation.

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