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Flow cytometry data analysis, James V. Watson



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Цена: 4370р.
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Автор: James V. Watson
Название:  Flow cytometry data analysis   (Анализ данных в проточной цитометрии)
Издательство: Cambridge Academ
Классификация:
Научное оборудование и методы, лабораторное оборудование
Клеточная биология

ISBN: 0521019702
ISBN-13(EAN): 9780521019705
ISBN: 0-521-01970-2
ISBN-13(EAN): 978-0-521-01970-5
Обложка/Формат: Paperback
Страницы: 300
Вес: 0.44 кг.
Дата издания: 15.09.2005
Язык: English
Издание: New ed
Иллюстрации: 134 line figures 5 tones 41 tables
Размер: 230 x 155 x 19
Читательская аудитория: Professional & vocational
Подзаголовок: Basic concepts and statistics
Ссылка на Издательство: Link
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Поставляется из: Англии
Описание: Flow cytometry is now well established in research laboratories and is gaining increasing use in clinical medicine and pathology. The technique enables multiple simultaneous light scatter and fluorescence measurements to be made at the individual cell level at very rapid rates and results in very large quantities of data being collected. Data, however, is just a series of numbers which have to be converted to information which, in turn, must be shown to have meaning. This is the most important single aspect of flow cytometry but it has received relatively little attention. One of the frequently voiced advantages of the technology is that it produces ‘good statistics’ because large numbers of cells have been analysed. However, it is not very often that confidence limits are placed on results, hence the reader has little or no feel for the inherent variability in the information produced. This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analysing dynamic processes. All those who use flow cytometry in their research will find this book an invaluable guide to interpreting the data produced by flow cytometers.
Дополнительное описание: Subject: Experimental biology and biomedicine / Cell biology and developmental biology
Readership: cell biology, microscopy
Level: academic researchers
Format: 228 x 152 mm 298pp 134 line diagrams 5 half-tones 41 tables
Chapter Titles: 1. Introduction; 2. Fundamental concepts; 3. Probability functions; 4. Significance testing and fit criteria; 5. Regression analysis; 6. Flow cytometric sources of variation; 7. Immunofluorescence data; 8. DNA histogram analysis; 9. Cell cycle kinetics; 10. Dynamic cellular events; 11. Multi-variant analysis primer; 12. Epilogue; Appendices; References; Index.





Flow Cytometry: First Principles, 2nd Edition

Автор: Alice Longobardi Givan
Название: Flow Cytometry: First Principles, 2nd Edition
ISBN: 0471382248 ISBN-13(EAN): 9780471382249
Издательство: Wiley
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Цена: 8605 р.
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Описание: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry; Progresses with an informal, engaging lecture style from simple to more complex concepts; Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis; Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields. Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Flow Cytometry

Автор: Macey
Название: Flow Cytometry
ISBN: 1588296911 ISBN-13(EAN): 9781588296917
Издательство: Springer
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Цена: 16602 р.
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Описание: Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.

Flow Cytometry for Biotechnology

Автор: Sklar, Larry A.
Название: Flow Cytometry for Biotechnology
ISBN: 0195183142 ISBN-13(EAN): 9780195183146
Издательство: Oxford Academ
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Цена: 6097 р.
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Описание: Shows how flow cytometry is integrated into modern biotechnology. This volume deals with issues of content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, and more.

Practical Flow Cytometry 4 ed

Автор: Shapiro, Howard M.
Название: Practical Flow Cytometry 4 ed
ISBN: 0471411256 ISBN-13(EAN): 9780471411253
Издательство: Wiley
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Цена: 19404 р.
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Описание: Explains the science and discusses the vast biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. This text covers a broad spectrum of applications in molecular biology and biotechnology. It includes chapters on automated analysis of array technologies, compensation, and high-speed sorting.

Flow Cytometry and Cell Sorting

Автор: Radbruch
Название: Flow Cytometry and Cell Sorting
ISBN: 3540656308 ISBN-13(EAN): 9783540656302
Издательство: Springer
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Цена: 27345 р.
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Описание: Updated to include improvements and modifications, this text presents new protocols such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection.

Flow Cytometry for Biotechnology

Автор: Klar, Larry A.
Название: Flow Cytometry for Biotechnology
ISBN: 0195152344 ISBN-13(EAN): 9780195152340
Издательство: Oxford Academ
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Цена: 4773 р.
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Описание: Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, proteomics and protein-protein interactions, vaccine development, plant and reproductive biology, and more.

Essential Cytometry Methods,

Автор: Zbigniew Darzynkiewicz
Название: Essential Cytometry Methods,
ISBN: 0123750458 ISBN-13(EAN): 9780123750457
Издательство: Elsevier Science
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Цена: 6683 р.
Наличие на складе: Поставка под заказ.

Описание: Cytometry is characterization and measurement of cells and cellular constituents, most often usedto immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers todetect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manualthat will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool,offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench.

Flow Cytometry: Pract Approach

Автор: Ormerod
Название: Flow Cytometry: Pract Approach
ISBN: 0199638241 ISBN-13(EAN): 9780199638246
Издательство: Oxford Academ
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Цена: 13781 р.
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Описание: This handbook on flow cytometry provides an introduction and guide for those new to the field and a reference for experienced practitioners who want to investigate new techniques. This edition contains new information on quality control and clinical applications, particularly apoptosis research.

Flow Cytometry with Plant Cells

Автор: Dolezel
Название: Flow Cytometry with Plant Cells
ISBN: 3527314873 ISBN-13(EAN): 9783527314874
Издательство: Wiley
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Цена: 20213 р.
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Описание: Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.

Introduction to Flow Cytometry

Автор: James V. Watson
Название: Introduction to Flow Cytometry
ISBN: 0521611997 ISBN-13(EAN): 9780521611992
Издательство: Cambridge Academ
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Цена: 5981 р.
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Описание: Flow cytometry is a technique for measuring both scattered light and fluorescence from single cells at very rapid rates. Typically up to 5000 cells can be analysed per second. Using various fluorochromes this allows a cell population to be analysed for cells showing certain characteristics such as the presence of a particular enzyme, cellular constituent or other gene product. The information it can provide is invaluable in helping to diagnose certain cancers as well as aiding pure research into many aspects of cell biology such as the cell cycle and gene expression. This book describes the technology in a simple and direct way. The fundamental concepts upon which the technology is based are outlined and the book goes on to describe flow cytometers and what can be gained from using them in biology and medicine. The book gives a particularly detailed account of how artifactual results can arise and where ‘noise’ is generated. Anyone starting to use, or already using this technique, will need to read this book.

Cytometry. New Developments. 4 ed.2005

Автор: Darzynkiewicz
Название: Cytometry. New Developments. 4 ed.2005
ISBN: 0125641702 ISBN-13(EAN): 9780125641708
Издательство: Elsevier Science
Цена: 12326 р.
Наличие на складе: Невозможна поставка.

Описание: The chapters in "Cytometry MCB" volumes, including this 4th Edition, provide comprehensive description of particular cytometric methods and review their applications. Some chapters also describe new instrumentation and provide fundamental information on use of new fluorescent probes and on data analysis. Although the term "edition" suggests the update of earlier volumes, in fact, nearly all chapters of the 4th Edition are devoted to new topics. The authors were invited to present not only technical protocols, such as available in other methodology books that specialize in the protocol format, but also to discuss the aspects of the methodology that generally are not included in the protocols. Many chapters, thus, present the theoretical foundations of the dcribed methods, their applicability in experimental laboratory and clinical setting, common traps and pitfalls, problems with data interpretation, comparison with alternative assays, choice of the optimal assay, etc. Some chapters review applications of cytometry and complementary methodologies to particular biological problems or clinical tasks. The 34 chapters presented in "Cytometry 4th Edition" cover a wide range of diverse topics. Several chapters describe progress in technology of fluorescence measurement. The novel phenomenon of the surface-plasmon coupled emission (SPCE) presented in one of these chapters, in combination with nanophotonic technology, is expected to open entirely new possibilities for ultra-sensitive detection of fluorescence, likely from single fluorochrome molecules. The chapter on cytometry of fluorescence resonance energy transfer (FRET) describes the theoretical foundations- and uncovers further analytical possibilities- of this methodology. The chapter on fluorescent proteins is an exhaustive review of accomplishments and possibilities offered in this rapidly expanding field. The critical assessment of quantitative analytical capabilities of confocal microscopy, optimization of emission optics and further progress in development of laser scanning cytometry instrumentation are the topics of other chapters focused on methodology of fluorescence measurement. Another group of chapters describe variety of cytometric methods and their applications in studies of cell death, particularly by mode of apoptosis, mechanism of antitumor drug action, and DNA damage. The rapidly growing application of cytometry in phytoplankton is also assessed in great detail. The chapters on biohazard sorting and data analysis guidelines will be of interest to a variety of researchers in different fields. Cell immunophenotyping represents the most common application of cytometry, particularly in clinical setting. It is not surprising, therefore, that over a third of all chapters of this volume are devoted to methods and applications of multi-color immunophenotyping and probing immune functions of lymphocytes and phagocytes. The field of cytogenetics and molecular genetics is represented by several chapters. Presented are the methods for telomere length measurement and genomic array technology as well as multiplex amplifiable probe hybridization (MAPH) and multiplex ligation dependent probe amplification (MLPA), the latter to probe the copy number changes (deletions, multiplications) in genomic DNA. A very exhaustive is the chapter describing the use of subtelometric probes in studies of mental retardation. In tradition with the earlier "Cytometry" editions, the chapters were prepared by the researchers who either developed the described methods, contributed to their modification, or found new applications and have extensive experience in their use. The list of authors, thus, is a continuation of "Who's Who" directory in the field of cytometry.


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