Описание: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.
Автор: Ulrich Reineke; Mike Schutkowski (Eds.) Название: Epitope Mapping Protocols ISBN: 1934115177 ISBN-13(EAN): 9781934115176 Издательство: Springer Рейтинг: Цена: 13557 р. Наличие на складе: Есть (1 шт.) Описание: Given the versatile utility of the determination of epitopes, the need for a thorough collection of experimental protocols is clear. This title offers various applications, including approaches to antibody or B-cell epitope mapping and T-cell epitope mapping and a section on the profiling of antibody signatures in biological fluids.
Описание: Provides a thorough and current treatment of the structure, organisation and function of the basic tissue types of the body as well as the organ systems which are organised from the basic tissues. Presents a modern cell biological emphasis on the subject, whilst also bringing out the clinical correlations of histology.
Автор: McMillan Donald B. Название: Fish Histology / Female Reproductive Systems ISBN: 1402054157 ISBN-13(EAN): 9781402054150 Издательство: Springer Рейтинг: Цена: 24466 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Fish вЂ” including cyclostomes, elasmobranchs, and teleosts вЂ” display a wide variety of ways of coping with the stringent conditions they encounter in the aquatic environment. This diversity is especially evident in the female genital systems where some fish spawn profligate numbers of small eggs while others develop a few yolky eggs that may receive parental care. Parental care is carried to extremes in viviparity where eggs develop within the ovary or oviducts of the mother. This volume describes the myriad ways in which fish have approached problems of reproduction вЂ” it is an amply illustrated comparative study of the microscopic structure of the female genital systems of fish. The timing of its appearance is auspicious in that it coincides with the decline of the golden age of descriptive morphology. It is a compilation of thousands of micrographs вЂ” mostly electron micrographs вЂ” from classic works in the field and should prove valuable to investigators studying fish in areas such as ecology, physiology, and reproductive biology who may view histology as essential in their work but have little background in this area. It includes chapters on the origin of genital systems, the structure of ovarian follicles, mechanisms of ovulation, the cortical reaction, oviducts, oviparity, and amazing examples of viviparity.
Описание: An English translation of the text written nearly a century ago. The two volumes contain over 1000 original illustrations and provide useful information about the anatomical organization of the nervous system structure-function correlates, and the history of neuroscience.
Описание: Maps the detailed architectonic subdivisions of the cortical and subcortical areas in the macaque monkey brain using high-resolution magnetic resonance (MR) images and the corresponding histology sections. This atlas presents the mapping of the architectonic areas in the horizontal plane of sections with reference to the MRI in macaque monkeys.
Автор: Mokhtar Название: Fish Histology, From Cells To Organ ISBN: 1771885890 ISBN-13(EAN): 9781771885898 Издательство: Taylor&Francis Рейтинг: Цена: 14108 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This new volume provides up-to-date information that emphasizes the relationships and concepts by which cell and tissue structures of fish are inextricably linked with their function. The book also describes the most recent development in the sciences of fish histology. Covers the normal histology of six fish species, the book provides detailed information on the histology of all organs of teleosts and includes 130 original photomicrographs, tables, updated terminology, and expanded information, with over 100 in color. This new volume, Fish Histology: From Cells to Organs, provides up-to-date information that emphasizes the relationships and concepts by which cell and tissue structures of fish are inextricably linked with their function. The book also describes the most recent development in the sciences of fish histology. Histology is the discipline of biology that involves the microscopic examination of tissue sections in order to study their structure and correlate it with function. Histology can detect signs of disease not easily recognized on gross examination and can therefore be of interest in fish health supervision. With fish constituting nearly 60% of all vertebrate species and of major worldwide economic importance as a food source, the information presented here will be valuable. The volume begins with concise introduction into the histological techniques for fish sampling, followed by an accurate up-to-date description of fish tissues. A chapter is devoted to each organ and organ systems in fish body as well. In addition, the book includes particular diagrams to illustrate the structure of organs and to enhance the usefulness of the text. This volume is designed for use by veterinary medical scientists, researchers, biologists, ichthyologists, fish farmers, veterinarians working in fisheries and, of course, by comparative histologists who want to learn more about the fish world. As a further aid to learning and identification, numerous photomicrographs and electron micrographs accompany the text, with particular emphasis on diagrams and tables to summarize morphologic and functional features of cells, tissues, and organs.
Автор: Lo Benny K. C. Название: Antibody Engineering / Methods and Protocols ISBN: 1588290921 ISBN-13(EAN): 9781588290922 Издательство: Springer Рейтинг: Цена: 16362 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.
Автор: Connell Joe Название: RT-PCR Protocols ISBN: 0896038750 ISBN-13(EAN): 9780896038752 Издательство: Springer Цена: 8878 р. Наличие на складе: Поставка под заказ.
Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
Автор: Federico Maurizio Название: Lentivirus Gene Engineering Protocols ISBN: 1607615320 ISBN-13(EAN): 9781607615323 Издательство: Springer Рейтинг: Цена: 11219 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition, covers the most relevant issues and techniques of LV-based gene engineering. This practical guide is aimed at those who wish to know more about this vital area of study.
Описание: This book details the latest aspects of the culture of neural cells and explains the practical and theoretical considerations of the techniques involved. It features step-by-step, readily reproducible laboratory protocols and expert implementation advice.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 16829 р. Наличие на складе: Есть (3 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
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