Histology Protocols, Tim D. Hewitson; Ian A. Darby (Eds.)
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 20789 р. Наличие на складе: Есть (3 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Ulrich Reineke; Mike Schutkowski (Eds.) Название: Epitope Mapping Protocols ISBN: 1934115177 ISBN-13(EAN): 9781934115176 Издательство: Springer Рейтинг: Цена: 16746 р. Наличие на складе: Есть (1 шт.) Описание: Given the versatile utility of the determination of epitopes, the need for a thorough collection of experimental protocols is clear. This title offers various applications, including approaches to antibody or B-cell epitope mapping and T-cell epitope mapping and a section on the profiling of antibody signatures in biological fluids.
Автор: Federico Maurizio Название: Lentivirus Gene Engineering Protocols ISBN: 1607615320 ISBN-13(EAN): 9781607615323 Издательство: Springer Рейтинг: Цена: 13859 р. Наличие на складе: Поставка под заказ.
Описание: Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition, covers the most relevant issues and techniques of LV-based gene engineering. This practical guide is aimed at those who wish to know more about this vital area of study.
Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.
Описание: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.
Автор: Wiechmann Allan F., Wirsig-Wiechmann Celeste R. Название: Color Atlas of Xenopus laevis Histology ISBN: 1402073755 ISBN-13(EAN): 9781402073755 Издательство: Springer Цена: 18474 р. Наличие на складе: Поставка под заказ.
Описание: The Color Atlas of "Xenopus laevis" Histology provides the first central source on the microscopic anatomy of cells, tissues, and major organs of the adult South African clawed frog, Xenopus laevis. For many years, X. laevis has been a highly popular experimental animal model in many areas of research. The recent development of transgenic Xenopus technology offers the promise that this animal model will be utilized more than ever before. The purpose of this book is to provide the active researcher with a central source of high quality light microscopic color images of the tissues of X. laevis, to aid in the identification of the cells and tissues of interest. Major features of this atlas include: *277 large, colorful, high resolution light microscopic images of cells, structures, tissues and organs; *Clear, detailed labeling of important structures in all images; *Concise, easy-to-read descriptions of histological organization and functions; *Colorful three-dimensional illustrations that simplify complex structure;*Detailed presentation of cranial structures and organization; *Easy-to-use cross-referenced index; *Bundled CD/ROM containing all images for easy downloading.
Описание: An English translation of the text written nearly a century ago. The two volumes contain over 1000 original illustrations and provide useful information about the anatomical organization of the nervous system structure-function correlates, and the history of neuroscience.
Автор: Mokhtar Название: Fish Histology, From Cells To Organ ISBN: 1771885890 ISBN-13(EAN): 9781771885898 Издательство: Taylor&Francis Рейтинг: Цена: 18563 р. Наличие на складе: Поставка под заказ.
Описание: This new volume provides up-to-date information that emphasizes the relationships and concepts by which cell and tissue structures of fish are inextricably linked with their function. The book also describes the most recent development in the sciences of fish histology. Covers the normal histology of six fish species, the book provides detailed information on the histology of all organs of teleosts and includes 130 original photomicrographs, tables, updated terminology, and expanded information, with over 100 in color. This new volume, Fish Histology: From Cells to Organs, provides up-to-date information that emphasizes the relationships and concepts by which cell and tissue structures of fish are inextricably linked with their function. The book also describes the most recent development in the sciences of fish histology. Histology is the discipline of biology that involves the microscopic examination of tissue sections in order to study their structure and correlate it with function. Histology can detect signs of disease not easily recognized on gross examination and can therefore be of interest in fish health supervision. With fish constituting nearly 60% of all vertebrate species and of major worldwide economic importance as a food source, the information presented here will be valuable. The volume begins with concise introduction into the histological techniques for fish sampling, followed by an accurate up-to-date description of fish tissues. A chapter is devoted to each organ and organ systems in fish body as well. In addition, the book includes particular diagrams to illustrate the structure of organs and to enhance the usefulness of the text. This volume is designed for use by veterinary medical scientists, researchers, biologists, ichthyologists, fish farmers, veterinarians working in fisheries and, of course, by comparative histologists who want to learn more about the fish world. As a further aid to learning and identification, numerous photomicrographs and electron micrographs accompany the text, with particular emphasis on diagrams and tables to summarize morphologic and functional features of cells, tissues, and organs.
Описание: A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.
Автор: Connell Joe Название: RT-PCR Protocols ISBN: 0896038750 ISBN-13(EAN): 9780896038752 Издательство: Springer Цена: 10967 р. Наличие на складе: Поставка под заказ.
Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
Описание: A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysi
Описание: Offers an introduction to histology with images and illustrations within a navigation structure. This is an interactive, annotated digital atlas of over 700 micrographs, along with an accompanying review text.
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