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Protein Chromatography, Walls


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Цена: 23058.00р.
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При оформлении заказа до: 2025-07-28
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Автор: Walls
Название:  Protein Chromatography
Перевод названия: Уоллс: Хроматография белка
ISBN: 9781607619123
Издательство: Springer
Классификация:



ISBN-10: 1607619121
Обложка/Формат: Hardback
Страницы: 485
Вес: 1.15 кг.
Дата издания: 01.10.2010
Серия: Methods in Molecular Biology Vol. 681
Язык: English
Издание: 1st Ed.
Иллюстрации: 44 black & white tables, biography
Размер: 254.00 x 178.00 x 30.00
Читательская аудитория: Professional & vocational
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.


High Performance Liquid Chromatography in Phytochemical Analysis

Автор: Monika Waksmundzka-Hajnos, Joseph Sherma
Название: High Performance Liquid Chromatography in Phytochemical Analysis
ISBN: 142009260X ISBN-13(EAN): 9781420092608
Издательство: Taylor&Francis
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Цена: 49764.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание:

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field.

The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products.

Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.

Affinity Chromatography / Methods and Protocols

Автор: Bailon Pascal
Название: Affinity Chromatography / Methods and Protocols
ISBN: 0896036944 ISBN-13(EAN): 9780896036949
Издательство: Springer
Цена: 7540.00 р.
Наличие на складе: Поставка под заказ.

Protein Chromatography

Автор: Walls
Название: Protein Chromatography
ISBN: 1493964100 ISBN-13(EAN): 9781493964109
Издательство: Springer
Рейтинг:
Цена: 20962.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.


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