Recent Advances in Cytometry, Part B,103, Zbigniew Darzynkiewicz
Старое издание
Автор: Darzynkiewicz Название: Cytometry. New Developments. 4 ed.2005 ISBN: 0125641702 ISBN-13(EAN): 9780125641708 Издательство: Elsevier Science Цена: 17383.00 р. Наличие на складе: Невозможна поставка. Описание: The chapters in "Cytometry MCB" volumes, including this 4th Edition, provide comprehensive description of particular cytometric methods and review their applications. Some chapters also describe new instrumentation and provide fundamental information on use of new fluorescent probes and on data analysis. Although the term "edition" suggests the update of earlier volumes, in fact, nearly all chapters of the 4th Edition are devoted to new topics. The authors were invited to present not only technical protocols, such as available in other methodology books that specialize in the protocol format, but also to discuss the aspects of the methodology that generally are not included in the protocols. Many chapters, thus, present the theoretical foundations of the dcribed methods, their applicability in experimental laboratory and clinical setting, common traps and pitfalls, problems with data interpretation, comparison with alternative assays, choice of the optimal assay, etc. Some chapters review applications of cytometry and complementary methodologies to particular biological problems or clinical tasks. The 34 chapters presented in "Cytometry 4th Edition" cover a wide range of diverse topics. Several chapters describe progress in technology of fluorescence measurement. The novel phenomenon of the surface-plasmon coupled emission (SPCE) presented in one of these chapters, in combination with nanophotonic technology, is expected to open entirely new possibilities for ultra-sensitive detection of fluorescence, likely from single fluorochrome molecules. The chapter on cytometry of fluorescence resonance energy transfer (FRET) describes the theoretical foundations- and uncovers further analytical possibilities- of this methodology. The chapter on fluorescent proteins is an exhaustive review of accomplishments and possibilities offered in this rapidly expanding field. The critical assessment of quantitative analytical capabilities of confocal microscopy, optimization of emission optics and further progress in development of laser scanning cytometry instrumentation are the topics of other chapters focused on methodology of fluorescence measurement. Another group of chapters describe variety of cytometric methods and their applications in studies of cell death, particularly by mode of apoptosis, mechanism of antitumor drug action, and DNA damage. The rapidly growing application of cytometry in phytoplankton is also assessed in great detail. The chapters on biohazard sorting and data analysis guidelines will be of interest to a variety of researchers in different fields. Cell immunophenotyping represents the most common application of cytometry, particularly in clinical setting. It is not surprising, therefore, that over a third of all chapters of this volume are devoted to methods and applications of multi-color immunophenotyping and probing immune functions of lymphocytes and phagocytes. The field of cytogenetics and molecular genetics is represented by several chapters. Presented are the methods for telomere length measurement and genomic array technology as well as multiplex amplifiable probe hybridization (MAPH) and multiplex ligation dependent probe amplification (MLPA), the latter to probe the copy number changes (deletions, multiplications) in genomic DNA. A very exhaustive is the chapter describing the use of subtelometric probes in studies of mental retardation. In tradition with the earlier "Cytometry" editions, the chapters were prepared by the researchers who either developed the described methods, contributed to their modification, or found new applications and have extensive experience in their use. The list of authors, thus, is a continuation of "Who's Who" directory in the field of cytometry.
Автор: Ortolani, Claudio Название: Flow cytometry of hematological malignancies ISBN: 144433588X ISBN-13(EAN): 9781444335880 Издательство: Wiley Рейтинг: Цена: 18295.00 р. Наличие на складе: Поставка под заказ.
Описание: Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference
Автор: Hawley Название: Flow Cytometry Protocols ISBN: 1617379492 ISBN-13(EAN): 9781617379499 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.
Описание: Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation and apoptosis. In most labs, training and resources are not available to teach methods of laser flow cytometry.
Автор: Andreas Radbruch (Editor) Название: Flow Cytometry and Cell Sorting (Springer Lab Manuals) ISBN: 3642084923 ISBN-13(EAN): 9783642084928 Издательство: Springer Рейтинг: Цена: 36570.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago.
Автор: Alice Longobardi Givan Название: Flow Cytometry: First Principles, 2nd Edition ISBN: 0471382248 ISBN-13(EAN): 9780471382249 Издательство: Wiley Рейтинг: Цена: 12506.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book is a succinct, accessible, "tutorial" approach to exploring flow cytometry and its applications for the interested novice or non-specialist. This updated book touches on basic principles of instrumentation, analysis, and research, as well as diagnostic applications in biology and medicine.
Автор: McCarty Название: Cytometric Analysis of Cell Phenotype and Function ISBN: 0521660297 ISBN-13(EAN): 9780521660297 Издательство: Cambridge Academ Рейтинг: Цена: 30888.00 р. Наличие на складе: Поставка под заказ.
Описание: Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings, following improvements in instrument design and computing power and the increased availability of fluorescent agents. This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding and apoptosis. Guidance is given on data interpretation, quality control procedures, pitfalls and problems. All chapters contain detailed protocols from leading authorities in the field with extensive practical experience.
Автор: Ormerod Название: Flow Cytometry: Pract Approach ISBN: 0199638241 ISBN-13(EAN): 9780199638246 Издательство: Oxford Academ Рейтинг: Цена: 11563.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Flow cytometry is a technique widely used in biological research and in diagnostic medicine. Flow cytometers are found in most biological research institutions and most clinical laboratories in larger hospitals.
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