Описание: * Covers a new approach to gene therapy with a greatly improved safety profile * Complete coverage of the topic including molecular basis, production and biomedical applications * Has a wide audience including everyone who is directly or indirectly involved in the development of gene therapies .
Описание: This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles. Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools guides readers through methods on macromoleculedelivery and chapters describe the LV-based protocols of gene engineering. Written in thehighly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Lentiviral Vectors andExosomes as Gene and Protein Delivery Tools aims to ensure successfulresults in the further study of this vital field.
Автор: Candiani Название: Non-Viral Gene Delivery Vectors ISBN: 1493937162 ISBN-13(EAN): 9781493937165 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery—also termed reversetransfection—and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods andProtocols is written for experimentalists, and is an essential part of manylaboratory bookshelves. This book will help novice and professionals alikesucceed in their research in this field.
Описание: Describes methods for the production of viral and non-viral gene transfer vectors, as well as protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering.
Описание: The field of gene therapy has undergone remarkable advances, promising to impact human healthcare in the 21st century. This book provides methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression.
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