The Sarcomere and Skeletal Muscle Disease, Nigel G. Laing

Автор: Kyba
Название: Skeletal Muscle Regeneration in the Mouse
ISBN: 1493938088 ISBN-13(EAN): 9781493938087
Издательство: Springer
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Цена: 19564.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume focuseson the cell biology and physiology of skeletal muscle regeneration. This Bookis a collection of classic and cutting edge protocols optimized for mice, butin most cases adaptable to rat or other mammalian models, that will allow aninvestigator to develop and implement a research study on skeletal muscleregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury byeccentric contraction; volumetric muscle loss; single myofiber isolation andculture; satellite cell transplantation; muscle clearing for whole mountimmunostaining; luciferase tracking of muscle stem cells; mitochondrialand mitophagy flux analysis; in vivo assessment of muscle contractility;force measurements on single isolated myofibers; and analysis of aerobicrespiration in intact skeletal muscle tissue by microplate respirometry. Written in the highlysuccessful Methods in Molecular Biology series format,chapters include introductions to each respective topic, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge andpractical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research inskeletal muscle growth, damage, repair, degeneration, and regenerative therapyin the mouse model system.

Автор: Elizabeth A. DiGangi
Название: Research Methods in Human Skeletal Biology,
ISBN: 0123851890 ISBN-13(EAN): 9780123851895
Издательство: Elsevier Science
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Цена: 12462.00 р.
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Описание: Showing you how to conduct research in general, this title also reveals how research is specifically conducted within human skeletal biology. It outlines the advanced types of research being conducted within each sub-specialty of skeletal biology, and gives the reader the tools to set up a research project in skeletal biology.