Автор: Anthony Atala Название: Handbook of Stem Cells, vol.1-2 ISBN: 0123859425 ISBN-13(EAN): 9780123859426 Издательство: Elsevier Science Рейтинг: Цена: 29722.00 р. Наличие на складе: Есть (2 шт.) Описание: Offers a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations. This title also offers advanced information of what is known about each specific organ system.
Автор: Zhang Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells ISBN: 1607617684 ISBN-13(EAN): 9781607617686 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.
Автор: Dummies Название: Stem Cells For Dummies ISBN: 0470259280 ISBN-13(EAN): 9780470259283 Издательство: Wiley Рейтинг: Цена: 2374.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The first authoritative yet accessible guide to this controversial topic Stem Cell Research For Dummies offers a balanced, plain-English look at this politically charged topic, cutting away the hype and presenting the facts clearly for you, free from debate.
Автор: Hoffman Название: Multipotent Stem Cells of the Hair Follicle ISBN: 1493937847 ISBN-13(EAN): 9781493937844 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume discusses methods for the study of multipotent and pluripotentstem cells of the hair follicle. The stem cells described are involved in boththe growth of the hair follicle and its production of the hair shaft, as wellas the growth of the hair follicle sensory nerve. Multipotent Stem Cells ofthe Hair Follicle: Methods and Protocols also explores very unexpectedresults such as that of the hair follicle-associated-pluripotent (HAP) stemcells, which not only have the capability for regenerating the hair folliclesensory nerve, but also can differentiate ex vivo and in vivo to multiple celltypes not associated with the hair follicle—these include glial cells, motorneurons, and beating cardiac muscle cells. The potential for HAP stem cells forregenerative medicine is also discussed in detail. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Cutting-edgeand thorough, Multipotent Stem Cells of the Hair Follicle: Methods andProtocols is a valuable resource for researchers interested in thisfield.
Автор: Turksen Название: Adult Stem Cells. 2004 ISBN: 1588291529 ISBN-13(EAN): 9781588291523 Издательство: Springer Рейтинг: Цена: 28734.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 2-D Protein Gel Electrophoresis: An Overview, Jenny Fichmann and Reiner Westermeier. Solubilization of Proteins in 2-D Electrophoresis: An Outline, Thierry Rabilloud. Preparation of Escherichia coli Samples for 2-D Gel Analysis, Ruth A. VanBogelen and Frederick C. Neidhardt. Preparing 2-D Protein Extracts from Yeast, David M. Schieltz. 2-D Protein Extracts from Drosophila melanogaster, Christer Ericsson. Preparing 2-D Protein Extracts from Caenorhabditis elegans, Robert Zwilling. Eukaryotic Cell Labeling and Preparation for 2-D, Nick Bizios. Differential Detergent Fractionation of Eukaryotic Cells: Analysis by Two-Dimensional Gel Electrophoresis, Melinda L. Ramsby and Gregory S. Makowski. Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis, Joachim Klose. Preparation and Solubilization of Body Fluids for 2-D, Jean Charles Sanchez and Denis F. Hochstrasser. 2-D Electrophoresis of Plant Proteins, Akira Tsugita and Masaharu Kamo. Quantifying Protein in 2-D PAGE Solubilization Buffers, Louis S. Ramagli. Measuring the Radioactivity of 2-D Protein Extracts, Andrew J. Link and Nick Bizios. Advantages of Carrier Ampholyte IEF, Mary F. Lopez. 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF), Mary F. Lopez. Nonequilibrium pH Gel Electrophoresis (NEPHGE), Mary F. Lopez. High Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment, Marion Sarmiento. Large-Gel 2-D Electrophoresis, Joachim Klose. Advantages of Immobilized pH Gradients, Jenny Fichmann. Casting Immobilized pH Gradients (IPGs), Elisabetta Gianazza. Analytical IPG-Dalt, Angelika GГ¶rg and Walter Weiss. IPG-Dalt of Very Alkaline Proteins, Angelika GГ¶rg. Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D, Neil M. Matsui, Diana M. Smith-Beckerman, Jenny Fichmann, and Lois B. Epstein. In-Gel Sample Rehydration of Immobilized pH Gradient, Jean-Charles Sanchez, Denis Hochstrasser, and Thierry Rabilloud. High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis, Jean-Charles Sanchez and Denis F. Hochstrasser. Horizontal SDS-PAGE for IPG-Dalt, Angelika GГ¶rg and Walter Weiss. Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE, Bradley J. Walsh and Benjamin R. Herbert. Nonreducing 2-D Polyacrylamide Gel Electrophoresis, Hong Ji and Richard J. Simpson. 2-D Diagonal Gel Electrophoresis, Joan Goverman. 2-D Phosphopeptide Mapping, Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky, and Steven F. Dowdy. Internal Standards for 2-D, Andrew J. Link. Autoradiography of 2-D Gels, Andrew J. Link. Double-Label Analysis, Kelvin H. Lee and Michael G. Harrington. Silver Staining of 2-D Electrophoresis Gels, Thierry Rabilloud. Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining, Neil M. Matsui, Diana M. Smith-Beckerman, and Lois B. Epstein. Electroblotting of Proteins from 2-D Polyacrylamide Gels, Michael J. Dunn. Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels, Michael J. Dunn. Protein Detection Using Reversible Metal Chelate Stains, Wayne F. Patton, Mark J. Lim, and David Shepro. Gylcoprotein Detection of 2-D Separated Proteins, Nicolle H. Packer, Malcolm S. Ball, and Peter L. Devine. Image Acquisition in 2-D Electrophoresis, Wayne F. Patton, Mark J. Lim, and David Shepro. Computer Analysis of 2-D Images, Ron D. Appel and Denis F. Hochstrasser. 2-D Databases on the World Wide Web, Ron D. Appel, Amos Bairoch, and Denis F. Hochstrasser. Comparing 2-D Electrophoresis Gels Across Internet Databases, Peter F. Lemkin. Constructing a 2-D Database for the World Wide Web, Ron D. Appel, Christine Hoogland, Amos Bairoch, and Denis F. Hochstrasser. Absolute Quantitation of 2-D Protein Spots, Steven P. Gygi and Ruedi Aebersold. Generating a Bacterial Genome Inventory: Identifying 2-D Spots by Comigrating Products of the Genome on 2-D Gels, Ruth A. VanBogelen. Immunoaffinity Identification of 2-DE Separated Proteins, Barbara Magi, Luca Bini, Barbara Marzocchi, Sabrina Liberatori, Roberto Raggiaschi, and Vitaliano Pallini. 2-DE Spot Amino Acid Analysis with 9-Fluroenylmethyl Chloroformate, Marc R. Wilkins, Jun X. Yan, and Andrew A. Gooley. N-Terminal Amino Acid Sequencing of 2-DE Spots, Masaharu Kamo and Akira Tsugita. Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategies for Microsample Handling, Hediye Erdjument-Bromage, Mary Lui, Lynne Lacomis, and Paul Tempst. Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry, Rachel R. Ogorzalek Loo, Joseph A. Loo, and Philip C. Andrews. Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses, Paul L. Courchesne and Scott D. Patterson. Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Protein Identification and Analysis Tools in the ExPASy Server, Marc R. Wilkins, Elisabeth Gasteiger, Amos Bairoch, Jean-Charles Sanchez, Keith L. Williams, Ron D. Appel, and Denis F. Hochstrasser. Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry, John R. Yates III, Edwin Carmack, Lara Hays, Andrew J. Link, and Jimmy K. Eng. Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Index.
Описание: Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense. With contributions from some of the leading researchers in the field, Male Germline Stem Cells: Developmental and Regenerative Potential assesses the implications of these discoveries for understanding the fundamental biology of germline stem cells as well as their potential for human stem cell-based therapies. This monograph covers many of the fundamental issues now being explored by today’s generation of stem cell researchers, including the field’s potential for regenerative medicine. Ranging from an assessment of the pluripotency of primordial germ cells and their possible applications in treating testicular cancer, to the recovery of once-mordant fertilization-competent sperm, this volume has it all. It is a reference point for any scientist involved in related research as well as being a timely summation of what could prove to be a hugely exciting and very fruitful area of inquiry.
Автор: Liu, Huiping Название: Cancer Stem Cells ISBN: 0128038926 ISBN-13(EAN): 9780128038925 Издательство: Elsevier Science Рейтинг: Цена: 19875.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Cancer Stem Cells: Targeting the Roots of Cancer, Seeds of Metastasis, and Sources of Therapy Resistance introduces the basic concepts and advanced understanding of cancer stem cells, covering general overviews, organ-specific identifications, and their characteristic mechanisms. The book also explores innovative therapeutic strategies in preclinical and clinical trials to target cancer stem cells, remove the roots of cancer, eliminate the seeds of metastasis, overcome the resistance of therapies, and contribute to the eradication of cancer.
The book includes contributions from leading, worldwide experts in the field, helping readers embrace new hope in their quest to eradicate cancer with emerging clinical trials on treating cancer stem cells in combination with other therapies.
Автор: Simon С. Название: Stem Cells in Human Reproduction. 2 ed.2008 ISBN: 0415471710 ISBN-13(EAN): 9780415471718 Издательство: Informa Рейтинг: Цена: 17157.00 р. Наличие на складе: Нет в наличии.
Автор: Slack Jonathan Название: Stem Cells ISBN: 0199603383 ISBN-13(EAN): 9780199603381 Издательство: Oxford Academ Рейтинг: Цена: 637.00 р. Наличие на складе: Нет в наличии.
Описание: There is much public interest in stem cells, but also much confusion and misinformation. In this Very Short Introduction, developmental biologist Jonathan Slack explains the biology behind stem cells; what they are, what scientists do with them, what stem cell therapies are available today, and what can be expected to happen in the future.
Автор: Buszczak Название: Germline Stem Cells ISBN: 1493940155 ISBN-13(EAN): 9781493940158 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter’s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.
Автор: Ainscough Название: Nuclear Reprogramming and Stem Cells ISBN: 1617792241 ISBN-13(EAN): 9781617792243 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply into the mechanisms that drive disease aetiology the research is realistically opening the doors for development of targeted and personalized therapeutic applications that many considered, until recently, to be nothing more that a far fetched dream. This volume provides a timely glimpse into the methods that have been developed to instigate, and the mechanisms that have been identified to drive, the process of nuclear reprogramming, chronicling how the field has developed over the last 50-60 years. Since the early 1950s a small number of notable experiments have provided significant impetus to the field, primarily the demonstration of reprogramming ability, first by the complex cytoplasmic milieu that constitutes the amphibian egg, then that of the mammalian egg, and finally that of the mammalian embryonic stem cell. Most recently, the demonstration that a limited pool of defined molecules is capable of reprogramming a multitude of cell types has provided massive impetus and facilitated transition towards realistic therapeutic application. We have therefore reproduced some of the key articles that elegantly document these dramatic stages of development of the field in an inclusive appendix to the book, for the benefit of readers keen to investigate the history of how the field of stem cell biology has evolved. Owing to the ever broadening nature of this field, and the incredible rate at which it is evolving, the main content of this volume focuses on areas that have shown significant movement in recent years, are most likely to translate into personalized therapeutic application, and thus provide greatest potential for significant impact on human health in the not too distant future. We recognize that research into many other disease states and cell types are all equally worthy of discussion. We would therefore like to acknowledge those researchers involved whose work we have not been able to include in this volume. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the field.
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