Hybridization of Somatic Cells, Ephrussi Boris

Автор: Liehr
Название: Fluorescence In Situ Hybridization (FISH)
ISBN: 3662529572 ISBN-13(EAN): 9783662529577
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.

Название: FISH (Fluorescence in Situ Hybridization)
ISBN: 0199638845 ISBN-13(EAN): 9780199638840
Издательство: Oxford Academ
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Цена: 19008.00 р.
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Описание: FISH (Fluorescence In Situ Hybridization) is a versatile technique that is constantly being applied to branches of research. With over 100 protocols, this book will be of significant interest to students and technicians in both clinical service and basic science laboratories who employ genetic or genomic technologies.

Автор: Jain S. Mohan, Gupta Pramod K.
Название: Protocol for Somatic Embryogenesis in Woody Plants
ISBN: 1402029845 ISBN-13(EAN): 9781402029844
Издательство: Springer
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Цена: 32142.00 р.
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Описание: Earlier, we edited a well received series on "Somatic embryogenesis in woody trees", volumes 1 to 6. These volumes provided readers detailed extensive reviews on somatic embryogenesis of important angiosperm and gymnosperm tree species; an excellent source of information for newcomers or those already engaged in research. However, these book volumes did not cover stepwise "detailed protocols" for inducing somatic embryogenesis. This book contains 46 chapters, divided into 4 sections : A) 12 chapters on conifers, B) 14 chapters on fruits, C) 14 chapters on angiosperms, and D) 6 chapters on histology, bioencapsulation, protoplasts, cryopreservation, double staining and thin cell layer sectioning. The book provides stepwise protocols for somatic embryogenesis of a range of selected woody plants in order to assist researchers to initiate somatic embryogenic cultures without too much alteration in protocols. Each chapter provides information on initiation and maintenance of embryogenic cultures; somatic embryo development, maturation, and germination; acclimatization and field transfer of somatic seedlings. Some chapters include applications of somatic embryogenic cultures, e.g. protoplasts, encapsulation, cryopreservation, genetic transformation, genetic fidelity with molecular markers, and bioreactor.