Adult Stem Cells, Paolo Di Nardo; Sanjiv Dhingra; Dinender K. Singla

Автор: Anthony Atala
Название: Handbook of Stem Cells, vol.1-2
ISBN: 0123859425 ISBN-13(EAN): 9780123859426
Издательство: Elsevier Science
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Описание: Offers a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations. This title also offers advanced information of what is known about each specific organ system.

Автор: Ainscough
Название: Nuclear Reprogramming and Stem Cells
ISBN: 1617792241 ISBN-13(EAN): 9781617792243
Издательство: Springer
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Описание: Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply into the mechanisms that drive disease aetiology the research is realistically opening the doors for development of targeted and personalized therapeutic applications that many considered, until recently, to be nothing more that a far fetched dream. This volume provides a timely glimpse into the methods that have been developed to instigate, and the mechanisms that have been identified to drive, the process of nuclear reprogramming, chronicling how the field has developed over the last 50-60 years. Since the early 1950s a small number of notable experiments have provided significant impetus to the field, primarily the demonstration of reprogramming ability, first by the complex cytoplasmic milieu that constitutes the amphibian egg, then that of the mammalian egg, and finally that of the mammalian embryonic stem cell. Most recently, the demonstration that a limited pool of defined molecules is capable of reprogramming a multitude of cell types has provided massive impetus and facilitated transition towards realistic therapeutic application. We have therefore reproduced some of the key articles that elegantly document these dramatic stages of development of the field in an inclusive appendix to the book, for the benefit of readers keen to investigate the history of how the field of stem cell biology has evolved.  Owing to the ever broadening nature of this field, and the incredible rate at which it is evolving, the main content of this volume focuses on areas that have shown significant movement in recent years, are most likely to translate into personalized therapeutic application, and thus provide greatest potential for significant impact on human health in the not too distant future. We recognize that research into many other disease states and cell types are all equally worthy of discussion. We would therefore like to acknowledge those researchers involved whose work we have not been able to include in this volume. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the field.

Автор: Juan Dom?nguez-Bendala
Название: Pancreatic Stem Cells
ISBN: 1617794864 ISBN-13(EAN): 9781617794865
Издательство: Springer
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Описание: This review of pancreatic development and regeneration provides a broad overview of organogenesis from the perspective of stem cell research. It is an all-encompassing guide to explore the many aspects of "regenerative therapies" for the endocrine pancreas.

Автор: Kursad Turksen
Название: Adult and Embryonic Stem Cells
ISBN: 162703952X ISBN-13(EAN): 9781627039529
Издательство: Springer
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Описание: Collecting reviews first presented at the 2010 Stem Cell Symposia in Samsun, Turkey, this book covers embryonic and adult stem cell sources. Reviews the current state of knowledge as well as the challenges that underlie translational medicine applications.

Автор: Turksen
Название: Adult Stem Cells. 2004
ISBN: 1588291529 ISBN-13(EAN): 9781588291523
Издательство: Springer
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Описание: 2-D Protein Gel Electrophoresis: An Overview, Jenny Fichmann and Reiner Westermeier. Solubilization of Proteins in 2-D Electrophoresis: An Outline, Thierry Rabilloud. Preparation of Escherichia coli Samples for 2-D Gel Analysis, Ruth A. VanBogelen and Frederick C. Neidhardt. Preparing 2-D Protein Extracts from Yeast, David M. Schieltz. 2-D Protein Extracts from Drosophila melanogaster, Christer Ericsson. Preparing 2-D Protein Extracts from Caenorhabditis elegans, Robert Zwilling. Eukaryotic Cell Labeling and Preparation for 2-D, Nick Bizios. Differential Detergent Fractionation of Eukaryotic Cells: Analysis by Two-Dimensional Gel Electrophoresis, Melinda L. Ramsby and Gregory S. Makowski. Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis, Joachim Klose. Preparation and Solubilization of Body Fluids for 2-D, Jean Charles Sanchez and Denis F. Hochstrasser. 2-D Electrophoresis of Plant Proteins, Akira Tsugita and Masaharu Kamo. Quantifying Protein in 2-D PAGE Solubilization Buffers, Louis S. Ramagli. Measuring the Radioactivity of 2-D Protein Extracts, Andrew J. Link and Nick Bizios. Advantages of Carrier Ampholyte IEF, Mary F. Lopez. 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF), Mary F. Lopez. Nonequilibrium pH Gel Electrophoresis (NEPHGE), Mary F. Lopez. High Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment, Marion Sarmiento. Large-Gel 2-D Electrophoresis, Joachim Klose. Advantages of Immobilized pH Gradients, Jenny Fichmann. Casting Immobilized pH Gradients (IPGs), Elisabetta Gianazza. Analytical IPG-Dalt, Angelika GГ¶rg and Walter Weiss. IPG-Dalt of Very Alkaline Proteins, Angelika GГ¶rg. Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D, Neil M. Matsui, Diana M. Smith-Beckerman, Jenny Fichmann, and Lois B. Epstein. In-Gel Sample Rehydration of Immobilized pH Gradient, Jean-Charles Sanchez, Denis Hochstrasser, and Thierry Rabilloud. High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis, Jean-Charles Sanchez and Denis F. Hochstrasser. Horizontal SDS-PAGE for IPG-Dalt, Angelika GГ¶rg and Walter Weiss. Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE, Bradley J. Walsh and Benjamin R. Herbert. Nonreducing 2-D Polyacrylamide Gel Electrophoresis, Hong Ji and Richard J. Simpson. 2-D Diagonal Gel Electrophoresis, Joan Goverman. 2-D Phosphopeptide Mapping, Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky, and Steven F. Dowdy. Internal Standards for 2-D, Andrew J. Link. Autoradiography of 2-D Gels, Andrew J. Link. Double-Label Analysis, Kelvin H. Lee and Michael G. Harrington. Silver Staining of 2-D Electrophoresis Gels, Thierry Rabilloud. Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining, Neil M. Matsui, Diana M. Smith-Beckerman, and Lois B. Epstein. Electroblotting of Proteins from 2-D Polyacrylamide Gels, Michael J. Dunn. Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels, Michael J. Dunn. Protein Detection Using Reversible Metal Chelate Stains, Wayne F. Patton, Mark J. Lim, and David Shepro. Gylcoprotein Detection of 2-D Separated Proteins, Nicolle H. Packer, Malcolm S. Ball, and Peter L. Devine. Image Acquisition in 2-D Electrophoresis, Wayne F. Patton, Mark J. Lim, and David Shepro. Computer Analysis of 2-D Images, Ron D. Appel and Denis F. Hochstrasser. 2-D Databases on the World Wide Web, Ron D. Appel, Amos Bairoch, and Denis F. Hochstrasser. Comparing 2-D Electrophoresis Gels Across Internet Databases, Peter F. Lemkin. Constructing a 2-D Database for the World Wide Web, Ron D. Appel, Christine Hoogland, Amos Bairoch, and Denis F. Hochstrasser. Absolute Quantitation of 2-D Protein Spots, Steven P. Gygi and Ruedi Aebersold. Generating a Bacterial Genome Inventory: Identifying 2-D Spots by Comigrating Products of the Genome on 2-D Gels, Ruth A. VanBogelen. Immunoaffinity Identification of 2-DE Separated Proteins, Barbara Magi, Luca Bini, Barbara Marzocchi, Sabrina Liberatori, Roberto Raggiaschi, and Vitaliano Pallini. 2-DE Spot Amino Acid Analysis with 9-Fluroenylmethyl Chloroformate, Marc R. Wilkins, Jun X. Yan, and Andrew A. Gooley. N-Terminal Amino Acid Sequencing of 2-DE Spots, Masaharu Kamo and Akira Tsugita. Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategies for Microsample Handling, Hediye Erdjument-Bromage, Mary Lui, Lynne Lacomis, and Paul Tempst. Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry, Rachel R. Ogorzalek Loo, Joseph A. Loo, and Philip C. Andrews. Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses, Paul L. Courchesne and Scott D. Patterson. Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Protein Identification and Analysis Tools in the ExPASy Server, Marc R. Wilkins, Elisabeth Gasteiger, Amos Bairoch, Jean-Charles Sanchez, Keith L. Williams, Ron D. Appel, and Denis F. Hochstrasser. Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry, John R. Yates III, Edwin Carmack, Lara Hays, Andrew J. Link, and Jimmy K. Eng. Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Index.

Автор: Slack Jonathan
Название: Stem Cells
ISBN: 0199603383 ISBN-13(EAN): 9780199603381
Издательство: Oxford Academ
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Цена: 637.00 р.
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Описание: There is much public interest in stem cells, but also much confusion and misinformation. In this Very Short Introduction, developmental biologist Jonathan Slack explains the biology behind stem cells; what they are, what scientists do with them, what stem cell therapies are available today, and what can be expected to happen in the future.

Автор: Robert Lanza
Название: Essentials of Stem Cell Biology,
ISBN: 0124095038 ISBN-13(EAN): 9780124095038
Издательство: Elsevier Science
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Цена: 26444.00 р.
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Описание: Suitable for scientists, researchers, practitioners, and students embracing the advances in stem cells, this book covers basic biology/mechanisms, early development, ectoderm, mesoderm, endoderm, and methods to the application of stem cells to specific human diseases, regulation and ethics, and patient perspectives.

Автор: John Morser; S. -I. Nishikawa; H.R. Schoeler
Название: Stem Cells in Reproduction and in the Brain
ISBN: 3642421598 ISBN-13(EAN): 9783642421594
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Expectations of the potential of regenerative medicine have risen recently because of exciting research results. This book contains contributions from leading researchers who describe their successes and the problems that remain in converting the hopes into concrete therapies.

Автор: Turksen Kursad
Название: Skin Stem Cells
ISBN: 1627033297 ISBN-13(EAN): 9781627033299
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This Methods in Molecular Biology(t) book details methods which are now commonly used to study skin stem cells. These include protocols, materials lists, tips and techniques for the isolation, maintenance and characterization of stem cell populations from skin.

Автор: Kyle E. Orwig; Brian P. Hermann
Название: Male Germline Stem Cells: Developmental and Regenerative Potential
ISBN: 1617797251 ISBN-13(EAN): 9781617797255
Издательство: Springer
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Описание: Conveying the excitement being generated by this cutting-edge field of specialist genetic inquiry, this wide-ranging survey features contributions from leading researchers and covers many key issues, including the cells` potential use in regenerative medicine.

Автор: John R. Masters (Editor), Bernhard O. Palsson (Edi
Название: Embryonic Stem Cells (Human Cell Culture)
ISBN: 1402059825 ISBN-13(EAN): 9781402059827
Издательство: Springer
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Описание: If you wish to grow or characterize embryonic stem cells or persuade them to differentiate into a particular cell type, then this book contains information that is vital to your success.

Название: Neural Stem Cells
ISBN: 0896039641 ISBN-13(EAN): 9780896039643
Издательство: Wiley
Цена: 13853.00 р.
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Описание: Neural Stem Cells: Methods and Protocols comprehensively addressed the major methods and techniques associated with current research in this field. Chapters cover topics such as detailed techniques for isolation, characterization and utilization of neural stem cells. The book provides a practical guide to laboratory procedures for neuroscientists, research clinicians, molecular biologists and geneticists as well as biology and medical students.