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Epstein Barr Virus, Janos Minarovits; Johann Helmut Niller


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Цена: 18167.00р.
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Автор: Janos Minarovits; Johann Helmut Niller
Название:  Epstein Barr Virus
ISBN: 9781493966530
Издательство: Springer
Классификация:

ISBN-10: 1493966537
Обложка/Формат: Hardback
Страницы: 269
Вес: 0.73 кг.
Дата издания: 2017
Серия: Methods in molecular biology
Язык: English
Издание: 1st ed. 2017
Иллюстрации: 33 tables, color; 21 illustrations, color; 1 illustrations, black and white; xiv, 269 p. 22 illus., 21 illus. in color.
Размер: 254 x 178 x 18
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
Дополнительное описание: Current Trends and Alternative Scenarios in EBV Research.- Epstein-Barr Virus: Clinical Diagnostics.- Establishment of EBV-Infected Lymphoblastoid Cell Lines.- Generation and Infection of Organotypic Cultures with Epstein-Barr Virus.- Affinity Purificatio



Epstein-Barr Virus Protocols

Автор: Wilson Joanna B., May Gerhard H. W.
Название: Epstein-Barr Virus Protocols
ISBN: 0896036901 ISBN-13(EAN): 9780896036901
Издательство: Springer
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Цена: 25853.00 р.
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Описание: Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer.

Human Immunodeficiency Virus Reverse Transcriptase

Название: Human Immunodeficiency Virus Reverse Transcriptase
ISBN: 1461472903 ISBN-13(EAN): 9781461472902
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This book covers coordination of RT-associated DNA polymerase and ribonuclease H activities, mechanisms of action and perspectives on NRTIs and NNRTIs, strategies for targeting the RT enzyme and mechanisms in HIV variability and development of drug resistance.


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