Nilles Type 3 Secretion Systems 9781493966479

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Автор: Nilles
Название: Type 3 Secretion Systems
ISBN: 9781493966479
Издательство: Springer
Классификация:

Молекулярная биология

Микробиология (немедицинская)

ISBN-10: 1493966472
Обложка/Формат: Hardback
Страницы: 224
Вес: 0.65 кг.
Дата издания: 2017
Серия: Methods in Molecular Biology
Язык: English
Издание: 1st ed. 2017
Иллюстрации: 16 tables, color; 23 illustrations, color; 9 illustrations, black and white; x, 224 p. 32 illus., 23 illus. in color.
Размер: 254 x 178 x 14
Читательская аудитория: Professional & vocational
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
Дополнительное описание: Introduction to Type III Secretion Systems.- Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components.- Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model.- In Vivo Photo-Cross-

Type 3 Secretion Systems, Nilles