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High-Throughput Glycomics and Glycoproteomics, Gordan Lauc


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Автор: Gordan Lauc
Название:  High-Throughput Glycomics and Glycoproteomics
Перевод названия: Лаук: Высокопроизводительная индустрия гликанов
ISBN: 9781493964918
Издательство: Springer
Классификация:


ISBN-10: 1493964917
Обложка/Формат: Mixed media product
Страницы: 274
Вес: 0.77 кг.
Дата издания: 2017
Серия: Methods in Molecular Biology
Язык: English
Издание: 1st ed. 2017
Иллюстрации: 42 illustrations, color; 9 illustrations, black and white; xi, 274 p. 51 illus., 42 illus. in color. with online files/update.
Размер: 265 x 187 x 22
Читательская аудитория: Professional & vocational
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.
Дополнительное описание: Ubiquitous Importance of Protein Glycosylation.- Laboratory Experimental Design for a Glycomic Study.- High-Throughput Analysis of the IgG N-Glycome by UPLC-FLR.- High-Throughput Analysis of IgG Fc Glycopeptides by LC-MS.- Sialic Acid Derivatization for t



Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing

Автор: Aransay
Название: Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing
ISBN: 3319313487 ISBN-13(EAN): 9783319313481
Издательство: Springer
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Цена: 23508.00 р.
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Описание: High throughput sequencing (HTS) technologies have conquered the genomics and epigenomics worlds. The applications of HTS methods are wide, and can be used to sequence everything from whole or partial genomes, transcriptomes, non-coding RNAs, ribosome profiling, to single-cell sequencing. Having such diversity of alternatives, there is a demand for information by research scientists without experience in HTS that need to choose the most suitable methodology or combination of platforms and to define their experimental designs to achieve their specific objectives. Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing aims to collect in a single volume all aspects that should be taken into account when HTS technologies are being incorporated into a research project and the reasons behind them. Moreover, examples of several successful strategies will be analyzed to make the point of the crucial features. This book will be of use to all scientist that are unfamiliar with HTS and want to incorporate such technologies to their research.


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