Plant Gene Transfer and Expression Protocols, Heddwyn Jones
Автор: Balbas Paulina, Lorence Argelia Название: Recombinant Gene Expression / Reviews and Protocols ISBN: 1588292622 ISBN-13(EAN): 9781588292629 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.
Автор: Peter E. Vaillancourt Название: E. coli Gene Expression Protocols ISBN: 1617373028 ISBN-13(EAN): 9781617373022 Издательство: Springer Рейтинг: Цена: 25853.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: coli vectors and strains for production of pure, functional protein, and the use of E. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.
Автор: David W. Murhammer Название: Baculovirus and Insect Cell Expression Protocols ISBN: 1617376299 ISBN-13(EAN): 9781617376290 Издательство: Springer Рейтинг: Цена: 25853.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology(TM) series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.
Автор: Paul J. Verma Название: Nuclear Transfer Protocols ISBN: 1617374687 ISBN-13(EAN): 9781617374685 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer.
Автор: K?re Lehmann Nielsen Название: Serial Analysis of Gene Expression (SAGE) ISBN: 1617377120 ISBN-13(EAN): 9781617377129 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book facilitates the introduction of SAGE into the laboratory and provides a framework for interpreting and comparing data derived from SAGE experiments. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world.
Автор: Orna Resnekov; Alexander von Gabain Название: Post-transcriptional Control of Gene Expression ISBN: 3642646093 ISBN-13(EAN): 9783642646096 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Many important cellular processes rely on posttranscriptional control of gene expression. Several introductory chapters discuss the general principles of translation and mRNA stability. The interactions of mature mRNA with the translational machinery, the components of mRNA degradation and antisense RNA are surveyed.
Автор: John F. Atkins; Raymond F. Gesteland Название: Recoding: Expansion of Decoding Rules Enriches Gene Expression ISBN: 146142531X ISBN-13(EAN): 9781461425311 Издательство: Springer Рейтинг: Цена: 28732.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Knowledge of recoding is essential for understanding the organization and expression of genes in viruses and all organisms. This volume details specific recoding examples to assist the reader with understanding the mechanisms and the mRNA signals.
Автор: Jac A. Nickoloff Название: Plant Cell Electroporation And Electrofusion Protocols ISBN: 1489940804 ISBN-13(EAN): 9781489940803 Издательство: Springer Рейтинг: Цена: 16979.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Gene transfer is an essential technology for improving our under- standing of gene structure and function. Although there are many meth- ods by which DNA may be introduced into cells--including heat and chemical treatments, and microinjection--electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi- ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in- cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna- tives to electric field-based methods, citing the advantages and limita- tions of the various methods for achieving specific goals.
Описание: This book seeks to emphasize the importance of molecular technology in the study of neurogenetic mechanisms and to explore the possible relationships between specific cell- cell interactions and regulated gene expression in the develop- ing nervous ~stem.
Автор: M. Karin Название: Gene Expression ISBN: 1468468138 ISBN-13(EAN): 9781468468137 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Fortunately, however, research during the past seven years on the mechanisms that control gene expression in eukaryotes has been extremely successful in generating a wealth of information on the basic strategies of transcriptional control.
ООО "Логосфера " Тел:+7(495) 980-12-10 www.logobook.ru
