Lipidomics of Stem Cells, Alice P?bay; Raymond C.B. Wong
Автор: Sanjoy K. Bhattacharya Название: Lipidomics ISBN: 1493969951 ISBN-13(EAN): 9781493969951 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Lipid Sample Preparation for Biomedical Research Ravin Sajnani and Katyayini Aribindi 2. Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays Kyle H. Elliot, James D. Roth, and Kevin Crook 3. Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells Vineet K. Gupta and Sulagna Banerjee 4. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation Blake E. Hopiavuori, Dustin E. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman 5. Sample Preparation and Analysis for Imaging Mass Spectrometry Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Paul Kowalski, and Sanjoy K. Bhattacharya 6. Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay Based Method Christopher E. Pedigo, Sandra M. Merscher, and Alessia Fornoni 7. High-Performance Chromatographic Separation of Cerebrosides Renaud Sicard and Ralf Landgraf 8. Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography Gabriel B. Gugiu 9. Utility of Moderate and High Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification Maria del Carmen Piqueras 10. A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry Candice Z. Ulmer, Rainey Patterson, Jeremy Koelmel, Timothy J. Garrett, and Richard A. Yost 11. Combined Use of MALDI-TOF Mass Spectrometry and 31PNMR Spectrometry for Analysis of Phospholipids Jenny Schrцter, Yulia Popkova, Rosmarie Sь , and Jьrgen Schiller 12. Global Monitoring of Mammalian Lipidome by Quantitative Shotgun Lipodomics Inger dum Nielsen, Kenji Maeda, and Mesut Bilgin13. Bioinformatics Pertinent to Lipid Analysis in Biological Samples Justin Ma, Ulises Arbelo, Yenifer Guerra, Katyayini Aribindi, Sanjoy K. Bhattacharya, and Daniel Pelaez 14. LC-MS-Based Lipidomics and Automated Identification of Lipids using the LipidBlast In-Silico MS/MS Library Tomas Cajka and Oliver Fiehn 15. Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa Pallavi Lahiri and Dipankar Ghosh 16. Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains Mark E. McClellan and Michael H. Elliott 17. Computational Functional Analysis of Lipid Metabolic Enzymes Carolina Bagnato, Arjen Ten Have, Marнa B. Prados, and Marнa B. Beligni 18. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells Evan B. Stubbs, Jr. 19. Purification and Validation of Lipid Transfer Proteins Matti A. Kjellberg, Anders P.E. Backman, Anna Mцuts, and Peter Mattjus 20. Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells Rania Leventis and John R. Silvius 21. Sonication-Based Basic Protocol for Liposome Synthesis Roberto Mendez and Santanu Banerjee 22. On Electrochemical Methods for Determination of Protein-Lipid Interaction Zhiping Hu and Yanli Mao 23. Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate Joseph L. Wilkerson and Nawajes A. Mandal
Автор: Paul Wood Название: Lipidomics ISBN: 1493969447 ISBN-13(EAN): 9781493969449 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes.
Автор: Xianlin Han Название: Lipidomics: Comprehensive Mass Spectrometry of Lipids ISBN: 1118893123 ISBN-13(EAN): 9781118893128 Издательство: Wiley Рейтинг: Цена: 19792.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Covers the area of lipidomics from fundamentals and theory to applications
Presents a balanced discussion of the fundamentals, theory, experimental methods and applications of lipidomics
Covers different characterizations of lipids including Glycerophospholipids; Sphingolipids; Glycerolipids and Glycolipids; and Fatty Acids and Modified Fatty Acids
Includes a section on quantification of Lipids in Lipidomics such as sample preparation; factors affecting accurate quantification; and data processing and interpretation
Details applications of Lipidomics Tools including for Health and Disease; Plant Lipidomics; and Lipidomics on Cellular Membranes
Описание: This book presents an overview of computational and statistical design and analysis of mass spectrometry-based proteomics, metabolomics, and lipidomics data. This contributed volume provides an introduction to the special aspects of statistical design and analysis with mass spectrometry data for the new omic sciences. The text discusses common aspects of design and analysis between and across all (or most) forms of mass spectrometry, while also providing special examples of application with the most common forms of mass spectrometry. Also covered are applications of computational mass spectrometry not only in clinical study but also in the interpretation of omics data in plant biology studies.Omics research fields are expected to revolutionize biomolecular research by the ability to simultaneously profile many compounds within either patient blood, urine, tissue, or other biological samples. Mass spectrometry is one of the key analytical techniques used in these new omic sciences. Liquid chromatography mass spectrometry, time-of-flight data, and Fourier transform mass spectrometry are but a selection of the measurement platforms available to the modern analyst. Thus in practical proteomics or metabolomics, researchers will not only be confronted with new high dimensional data types—as opposed to the familiar data structures in more classical genomics—but also with great variation between distinct types of mass spectral measurements derived from different platforms, which may complicate analyses, comparison, and interpretation of results.
Описание: This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf.Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics.The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.
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