3D Cell Culture, Zuzana Koledova

Автор: Helgason Cheryl D., Miller Cindy
Название: Basic Cell Culture Protocols
ISBN: 1588292843 ISBN-13(EAN): 9781588292841
Издательство: Springer
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Цена: 27951.00 р.
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Описание: In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Автор: Lodish H.
Название: Molecular Cell Biology. 7 ed. IE
ISBN: 1464109818 ISBN-13(EAN): 9781464109812
Издательство: Springer
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Цена: 13275.00 р.
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Описание: With its acclaimed authors, cutting-edge content, emphasis on medical relevance and landmark experiments, Molecular Cell Biology is an impeccable textbook. Updated throughout, the seventh edition features new co-author Angelika Amon, a completely rewritten chapter on the Cell Cycle and significant updates to experimental techniques.

Автор: Edited by Margaret J. Dallman
Название: Haematopoietic and Lymphoid Cell Culture
ISBN: 0521629691 ISBN-13(EAN): 9780521629690
Издательство: Cambridge Academ
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Цена: 8078.00 р.
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Описание: Detailed protocols and descriptive accounts of all the major haematopoietic and lymphoid cells written by experts with hands-on experience of potential pitfalls and tips for success. Designed to allow easy setting up of successful cultures by following step-by-step methods. A concise and affordable handbook for postgraduates, lab technicians and researchers.

Автор: Cree
Название: Cancer Cell Culture
ISBN: 1617790796 ISBN-13(EAN): 9781617790799
Издательство: Springer
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Цена: 32142.00 р.
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Описание: With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.