Metalloproteins, Juan C. Fontecilla-Camps; Yvain Nicolet

Автор: Ian M. Clark
Название: Matrix Metalloproteinase Protocols
ISBN: 1603272984 ISBN-13(EAN): 9781603272988
Издательство: Springer
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Цена: 14673.00 р. 20962.00 -30%
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Описание: Detailing proven laboratory techniques for the study of MMPs, this book covers the methods including those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances.

Автор: Juan C. Fontecilla-Camps; Yvain Nicolet
Название: Metalloproteins
ISBN: 1493963287 ISBN-13(EAN): 9781493963287
Издательство: Springer
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Цена: 14673.00 р.
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Описание:

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, M ssbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

Автор: Graeme Hanson; Lawrence Berliner
Название: Future Directions in Metalloprotein and Metalloenzyme Research
ISBN: 3319590987 ISBN-13(EAN): 9783319590981
Издательство: Springer
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Цена: 19564.00 р.
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Описание:

This book covers the latest developments in metalloenzymes, including characterizing metal bridging in proteins and peptides, copper(II) complexes of marine peptides, high-spin Co(II) in model and metalloprotein systems to enzymes such as the molybdenum-containing enzymes, CW and pulse EPR of cytochrome P450 enzymes and the radical S-adenosylmethionine FeS family. In the previous two related volumes in the Biological Magnetic Resonance series, High-Resolution EPR: Applications to Metalloenzymes and Metals in Medicine and Metals in Biology: Applications of High-Resolution EPR to Metalloenzymes, topics covered included high-resolution EPR methods, iron proteins, nickel and copper enzymes, metals in medicine, iron-sulfur cluster-containing proteins, and molybdenum enzymes. In this volume, new developments in these areas are covered in detail and new areas that have emerged are also detailed. This is an ideal book for graduate students and researchers working in the fields of high-resolution EPR, metalloenzymes, and metals in biology.