Elena Garc?a-Fruit?s Insoluble Proteins 9781493922048

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Автор: Elena Garc?a-Fruit?s
Название: Insoluble Proteins
ISBN: 9781493922048
Издательство: Springer
Классификация:

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ISBN-10: 1493922041
Обложка/Формат: Hardcover
Страницы: 425
Вес: 0.98 кг.
Дата издания: 02.12.2014
Серия: Methods in Molecular Biology
Язык: English
Размер: 254 x 178 x 25
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание:

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

Дополнительное описание: General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins.- Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production.- Optimization of Culture Parameters and Novel Strategies t

Insoluble Proteins, Elena Garc?a-Fruit?s