Описание: Part I: Selection
1. Selection of Aptamers for Metabolite Sensing and Construction of Optical Nanosensors
Yi Long, Franziska Pfeiffer, Gьnter Mayer, Tine Daa Schr der, Veli Cengiz Цzalp, and Lars Folke Olsen
2. SELEX of Cell-Specific RNA Aptamers
Katharina Berg, Eileen Magbanua, and Ulrich Hahn
3. Developing Aptamers by Cell-Based SELEX
Silvia Catuogno, Carla Lucia Esposito, and Vittorio de Franciscis
4. DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System
Michiko Kimoto, Ken-Ichiro Matsunaga, and Ichiro Hirao
5. Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C
Nasim Shahidi Hamedani and Jens Mьller
6. Preparation of SELEX Samples for Next-Generation Sequencing
Fabian Tolle and Gьnter Mayer
7. Next Generation Analysis of Deep Sequencing Data: Bringing Light into the Black Box of SELEX Experiments
Michael Blank
Part II: Characterization
8. Aptamer Binding Studies Using MicroScale Thermophoresis
Dennis Breitsprecher, Nina Schlinck, David Witte, Stefan Duhr, Philipp Baaske, and Thomas Schubert
9. Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry
Marc Vogel and Beatrix Suess
10. Applications of Aptamers in Flow and Imaging Cytometry
Isis C. Nascimento, Arthur A. Nery, Vinicius Bassaneze, Josй E. Krieger, and Henning Ulrich
11. In Vitro and In Vivo Imaging of Fluorescent Aptamers
Ioanna Thйodorou, Nam Nguyen Quang, Karine Gombert, Benoit Thйzй, Benoit Lelandais, and Frйdйric Ducongй
12. Crystallographic Pursuit of a Protein-RNA Aptamer Complex
John J.G. Tesmer
Part III: Application
13. Voltammetric Aptasensor Based on Magnetic Beads Assay for Detection of Human Activated Protein C
Arzum Erdem, Gulsah Congur, and Ece Eksin
14. Apta-PCR
Alessandro Pinto, Pedro Nadal Polo, Marketa Svobodova, Teresa Mairal Lerga, and Ciara K. OSullivan
15. Aptamer-Based Enzyme-Capture-Assay for Measurement of Plasma Thrombin Levels
Jens Mьller, Tobias Becher, Gьnter Mayer, and Bernd Pцtzsch
16. Application of Aptamers in Histopathology
Sarah Shigdar, Li Lv, Lifen Wang, and Wei Duan
17. Aptamer Stainings for Super-Resolution Microscopy
Maria Angela Gomes de Castro, Burkhard Rammner, and Felipe Opazo
18. Synthesis and Characterization of Aptamer Targeted SNALPs for the Delivery of siRNA
Samantha E. Wilner and Matthew Levy
19. Screening of Genetic Switches Based on the Twister Ribozyme Motif
Michele Felletti, Benedikt Klauser, and Jцrg S. Hartig
