Kinase Screening and Profiling, Hicham Zegzouti; Said A. Goueli

Автор: Thomas O. Metz
Название: Metabolic Profiling
ISBN: 149395816X ISBN-13(EAN): 9781493958160
Издательство: Springer
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Описание: This book presents protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. It features cutting-edge protocols, presented from the perspective of leading experts in the field.

Автор: Anton Posch
Название: Proteomic Profiling
ISBN: 1493925490 ISBN-13(EAN): 9781493925490
Издательство: Springer
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Описание: This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists.

Автор: Mathias Munschauer
Название: High-Resolution Profiling of Protein-RNA Interactions
ISBN: 3319162527 ISBN-13(EAN): 9783319162522
Издательство: Springer
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Описание: The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universit?t Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.

Автор: Oliver Schilling
Название: Protein Terminal Profiling
ISBN: 1493968491 ISBN-13(EAN): 9781493968497
Издательство: Springer
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Описание:

Preface...
Table of Contents...
Contributing Authors...

1. [¹⁴C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay
Adrian Drazic and Thomas Arnesen

2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity
Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen

3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation
Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel

4. Profiling of Protein N-Termini and their Modifications in Complex Samples
Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen

5. Protease Substrate Profiling by N-Terminal COFRADIC
An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens

6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification
Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss

7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types
Pascal Schlage and Ulrich auf dem Keller

8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates
Zon W. Lai and Oliver Schilling

9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC
Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme

10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling
Wenwen Duan and Guoqiang Xu

11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP)
R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements

12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling
Joanna Tucher and Andreas Tholey

13. FPPS - Fast Profiling of Protease Specificity
Matej Vizovisek, Robert Vidmar, and Marko Fonovic

14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics
Chia-yi Chen, Bettina Mayer, and Oliver Schilling

15. Prediction of Proteases Involved in Peptide Generation
Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein

16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches
Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane

17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors
Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber

18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method
Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger

19. Synthesis and Application of Activity-Based Probes for Proteases
Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst

Автор: Pawel Ciborowski
Название: Proteomic Profiling and Analytical Chemistry
ISBN: 0444636889 ISBN-13(EAN): 9780444636881
Издательство: Elsevier Science
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Описание:

"Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition" helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.

The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measureIncludes practical examples illustrating the importance of problems in quantitation and validation of biomarkersHelps in designing and executing proteomic experiments with sound analytics

Автор: Stephan A. Sieber
Название: Activity-Based Protein Profiling
ISBN: 3642448313 ISBN-13(EAN): 9783642448317
Издательство: Springer
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Описание: SieberActivity-Based Protein Profiling for Natural Product Target Discovery, by Joanna Krysiak und Rolf BreinbauerPhotoaffinity Labeling in Activity-Based Protein Profiling, by Paul P. TateFunctional Analysis of Protein Targets by Metabolomic Approaches, by Yun-Gon Kim und Alan Saghatelian

Автор: Peter K. Vogt; Stephen I. Reed
Название: Cyclin Dependent Kinase (CDK) Inhibitors
ISBN: 3642719430 ISBN-13(EAN): 9783642719431
Издательство: Springer
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Описание: More than 10 years ago, the discovery of cyclin-dependent ki- nases (Cdks) ushered in a new era in the understanding of cell proliferation and its control.

Автор: Aik-Choon Tan; Paul H. Huang
Название: Kinase Signaling Networks
ISBN: 1493971522 ISBN-13(EAN): 9781493971527
Издательство: Springer
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Цена: 27950.00 р.
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Описание: Beginning with a section on synthetic biology, chemical biology, and screening approaches to kinase signaling networks, the book continues with sections on mass spectrometry and metabolic analysis of kinase signaling as well as computational analysis of kinase signaling networks.