Heterologous Gene Expression in E.coli, Thomas C. Evans, Jr.; Ming-Qun Xu

Автор: Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493936352 ISBN-13(EAN): 9781493936359
Издательство: Springer
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Описание: This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Автор: Isabelle Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493961322 ISBN-13(EAN): 9781493961320
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein solubilization, purification, and instability in solution.

Автор: Paula Meleady
Название: Heterologous Protein Production in CHO Cells
ISBN: 1493969714 ISBN-13(EAN): 9781493969715
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using `omic approaches.

Автор: Peter E. Vaillancourt
Название: E. coli Gene Expression Protocols
ISBN: 1617373028 ISBN-13(EAN): 9781617373022
Издательство: Springer
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Описание: coli vectors and strains for production of pure, functional protein, and the use of E. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.

Автор: P.H. Hofschneider; W. Goebel
Название: Gene Cloning in Organisms Other Than E. coli
ISBN: 3642683177 ISBN-13(EAN): 9783642683176
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Gene cloning for the production of recombinant DNA is usually performed with E. coli. There is, however, no doubt that gene cloning in organisms other than E. coli will assume a much more important role in the future: efficient cloning systems are needed for the analysis of gene expression and its regulation in eukaryotic cells, for the elucidation ofthe genetic mechanisms of transform a- tion, and for the study of the genetic rearrangement during dif- ferentiation and embryogenesis. Furthermore, optimal cloning systems will be required for practical applications in the near future: for the optimal production of antibiotics, amino acids, vitamins, enzymes etc., for the expression of gene products under favorable energetic conditions for mass production, for the ex- pression of glycolysated animal proteins, for the genetic manipu- 'lation of plants -and in the more distant future, for gene therapy inman. The editors have therefore invited leading scientists to sum- marize the present status and the future applicability of gene cloning systems in their fields of research. To ensure a complete coverage of a genetic system, the editors have generally asked two authors to cover one system. This volume is a frrst and unique account of our knowledge of cloning systems employing organisms other than E. coli such as B. subtilis, pseudomonas, neurospora, saccharomyces and streptomyces, as well as of vectors and selection procedures for animal and plant systems including the liposome technique. October 1981 P. R.

Автор: Sang Yup Lee
Название: Systems Biology and Biotechnology of Escherichia coli
ISBN: 9048181186 ISBN-13(EAN): 9789048181186
Издательство: Springer
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Цена: 28732.00 р.
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Описание: This book, the first of its kind, brings together state-of-the-art reviews on systems biology and biotechnological applications of E. coli. Some of the many topics covered include genomics, network analysis, modeling and simulation, and protein synthesis.

Автор: Ranjan Chakraborty; Volkmar Braun; Klaus Hantke; P
Название: Iron Uptake in Bacteria with Emphasis on E. coli and Pseudomonas
ISBN: 9400760876 ISBN-13(EAN): 9789400760875
Издательство: Springer
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Цена: 7182.00 р.
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Описание: Chapter 2 details the variety of iron transport and iron regulatory systems of both gram negative and gram positive bacteria. This book is aimed at researchers in the fields of iron metabolism in multiple organisms, and to those who are interested in studying iron transport systems of bacteria.