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Metagenomics, Wolfgang R. Streit; Rolf Daniel


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Автор: Wolfgang R. Streit; Rolf Daniel
Название:  Metagenomics
ISBN: 9781493961238
Издательство: Springer
Классификация:

ISBN-10: 1493961233
Обложка/Формат: Paperback
Страницы: 341
Вес: 0.68 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 11 tables, black and white; xi, 341 p.
Размер: 254 x 178 x 19
Читательская аудитория: General (us: trade)
Основная тема: Biomedicine
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries. In Metagenomics: Methods and Protocols, expert researches provide an overview and introduction to basic methods commonly used in laboratories that have a strong background in microbial metagenomics. The book attempts to address all of the working steps involved in this crucial field, beginning with DNA isolation from soils and marine samples and continuing with the construction and screening of libraries, along with key advise involving bioinformatic tools available to analyze large metagenomic sequence data sets. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metagenomics: Methods and Protocols serves as a very complete guide to available screening protocols for all major biocatalysts in order to allow for the easy setup of these screens in any microbiology lab.
Дополнительное описание: Molecular Methods to Study Complex Microbial Communities.- Construction of Small-Insert and Large-Insert Metagenomic Libraries.- Construction and Screening of Marine Metagenomic Libraries.- Metagenomic Analysis of Isotopically Enriched DNA.- Wide Host-Ran



Metagenomics

Автор: Wolfgang Streit; Rolf Daniel
Название: Metagenomics
ISBN: 1493966898 ISBN-13(EAN): 9781493966899
Издательство: Springer
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Цена: 13974.00 р.
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Описание:

Preface...
Table of Contents...
Contributing Authors...


1. Construction of Small-Insert and Large-Insert Metagenomic Libraries
Carola Simon and Rolf Daniel
2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies
Dominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer

3. Construction and Screening Marine Metagenomic Large Insert Libraries
Nancy Weiland-Brдuer, Daniela Langfeldt, and Ruth A. Schmitz

4. Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment
Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard

5. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms
Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Цzge Eyice, Hendrik Schдfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont

6. Assessing Bacterial and Fungal Diversity in the Plant's Endosphere
Bernd Wemheuer and Franziska Wemheuer

7. Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities
James Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin

8. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production
Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Annй

9. Degradation Network Reconstruction Guided by Metagenomic Data
Rafael Bargiela and Manuel Ferrer

10. Novel Tools for the Functional Expression of Metagenomics DNA
Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger

11. A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries
Dominique Bцttcher, Patrick Zдgel, Marlen Schmidt, and Uwe T. Bornscheuer

12. Screening for Cellulase Encoding Clones in Metagenomics Libraries
Nele Ilmberger and Wolfgang R. Streit

13. Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes
Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade

14. Screening Glycosyltransferases for Polyphenol Modifications
Nele Ilmberger and Ulrich Rabausch

15. Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles

16. Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity
Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel

17. Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes
Nicole Adam and Mirjam Perner

18. Screening for N-AHSL-Based-Signaling Interfering Enzymes
Stйphane Uroz and Phil M. Oger

19. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites
F. Jerry Reen, Jose A. Gutiйrrez-Barranquero, and Fergal O'Gara

Handbook of Molecular Microbial Ecology II: Metagenomics in Different Habitats

Автор: Frans J. de Bruijn Frans J. de Bruijn
Название: Handbook of Molecular Microbial Ecology II: Metagenomics in Different Habitats
ISBN: 0470647191 ISBN-13(EAN): 9780470647196
Издательство: Wiley
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Цена: 41520.00 р.
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Описание: Metagenomics has revolutionized microbiology and many associated health and environmental fields. This is the first comprehensive treatise covering the various omics in a large variety of habitats, which could not previously have been analysed without metagenomic methodology.

Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches

Автор: Frans J. de Bruijn
Название: Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches
ISBN: 0470644796 ISBN-13(EAN): 9780470644799
Издательство: Wiley
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Цена: 45294.00 р.
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Описание: Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches is the first comprehensive reference covering the various metagenomics in a large variety of habitats, which could not previously have been analysed without metagenomic methodology.


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