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Protein Electrophoresis, Biji T. Kurien; R. Hal Scofield


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Автор: Biji T. Kurien; R. Hal Scofield
Название:  Protein Electrophoresis
ISBN: 9781493962419
Издательство: Springer
Классификация:



ISBN-10: 1493962418
Обложка/Формат: Paperback
Страницы: 648
Вес: 0.92 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 15 tables, black and white; 31 illustrations, color; 120 illustrations, black and white; xiv, 648 p. 151 illus., 31 illus. in color.
Размер: 234 x 156 x 34
Читательская аудитория: General (us: trade)
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.
Дополнительное описание: How It All Began -- A Personal History of Gel-Electrophoresis.- Introduction to Protein Electrophoresis.- Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis.- Protein Solubilization: Attend to the Choice of Lysis Bu



Microchip Capillary Electrophoresis Protocols

Автор: Ann Van Schepdael
Название: Microchip Capillary Electrophoresis Protocols
ISBN: 1493947745 ISBN-13(EAN): 9781493947744
Издательство: Springer
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Цена: 13275.00 р.
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Описание:

Part I. Overview

1. An Overview of the use of Microchips in Electrophoretic Separation Techniques: Fabrication, Separation Modes, Sample Preparation Opportunities, and On-Chip Detection

Stijn Hendrickx, Wim De Malsche, and Deirdre Cabooter

Part II. Microchip Capillary Electrophoresis Of Small Molecules

2. Microchip Electrophoresis for Fast and Interference-Free Determination of Trace Amounts of Glyphosate and Glufosinate Residues in Agricultural Products

Xuan Wei And Qiaosheng Pu

3. Microchip Capillary Electrophoresis Of Nitrite and Nitrate in Cerebrospinal Fluid

Mariбn Masбr, Rуbert Bodor, and Peter Troska

4. Analysis Of Thiols By Microchip Capillary Electrophoresis for In Situ Planetary Investigations

Maria F. Mora, Amanda M. Stockton, and Peter A. Willis

5. Determination of Ofloxacin in Ofloxacin Ear Drops by Microfluidic Chip with Contactless Conductivity Detection

Bing Chen and Kaicheng Li

Part III. Microchip Capillary Electrophoresis Of Nucleic Acids, Proteins, Peptides, Pathogens

6. Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation

Sona Krizkova, Hoai Viet Nguyen, Maja Stanisavljevic, Pavel Kopel, Marketa Vaculovicova, Vojtech Adam, and Rene Kizek

7. Fast High-Throughput Screening Of H1N1 Virus by Parallel Detection with Multi-Channel Microchip Electrophoresis

Peng Zhang, He Nan, Seungah Lee, and Seong Ho Kang

8. Sex Identification of Ancient DNA Samples using a Microfluidic Device

Kirsty J Shaw, Keri A. Brown, Terence A. Brown, and Stephen J. Haswell

9. Fast and Continuous-Flow Detection and Separation Of DNA-Complexes and DNA in Nanofluidic Chip Format

Martina Viefhues, Jan Regtmeier, and Dario Anselmetti

10. Multi-Dimension Microchip-Capillary Electrophoresis Device for Determination of Functional Proteins in Infant Milk Formula

Ruige Wu, Zhiping Wang, and Ying Sing Fung

11. Fast Assembly of Anti-Voltage Photonic Crystals in Microfluidic Channels for Ultrafast Separation of Amino Acids and Peptides

Yi Chen, Tao Liao and Can Hu

Part IV. Nonaqueous Applications

12. Rapid Determination of Catecholamines in Urine Samples by Nonaqueous Microchip Electrophoresis with LIF Detection

Hongmei Hu and Yuanming Guo

Part V. Capillary Electrochromatography On Microchips

13. Carbon Nanotube Based Separation Columns for Microchip Electrochromatography

K. B. Mogensen, B. Delacourt, and J. P. Kutter

14. Electrochromatography on Acrylate-Based Monolith in Cyclic Olefin Copolymer Microchip: an Attractive Technology

Y. Ladner, G. Cretier, and K. Faure

Part VI. Sample Preparation Approaches

15. On-Chip Electromembrane Extraction for Monitoring Drug Metabolism in Real Time by Electrospray Ionization Mass Spectrometry

Nickolaj J. Petersen, Henrik Jensen, and Stig Pedersen-Bjergaard

16. Sample Preparation for N-Glycosylation Analysis of Therapeutic Monoclonal Antibodies By Electrophoresis

Бkos Szekrйnyes, Jan Partyka, Csaba Varadi, Jana Krenkova, Frantisek Foret, and Andrбs Guttman

Capillary Electrophoresis of Carbohydrates

Автор: Nicola Volpi
Название: Capillary Electrophoresis of Carbohydrates
ISBN: 1627038302 ISBN-13(EAN): 9781627038300
Издательство: Springer
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Цена: 23757.00 р.
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Описание: Simple carbohydrates, complex oligosaccharides and polysaccharides all belong to a class of ubiquitous (macro)molecules that exhibit a wide range of biological functions, and the recent advent of enhanced enzymatic, chemical and analytical tools used to study these sugars has inaugurated a genuine explosion in the field of glycomics. Specifically, it has led to a deeper understanding of how specific sugar structures modulate cellular phenotypes, and that breakthrough has led to the discovery of new pharmaceuticals for the treatment of many serious diseases, such as cancer. The subsequent rapid expansion of this research holds high promise for future therapeutic regimens, and capillary electrophoresis (CE) refers to the range of related separation techniques that are integral to this vital research. CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules, and Capillary Electrophoresis of Carbohydrates offers a comprehensive look at the latest breakthroughs and improvements in CE and CE techniques applied to monosaccharides up to complex oligosaccharides and polysaccharides. It begins with an overview of the application of CE and CE- mass spectrometric in the analysis of simple carbohydrates without any previous derivatization step before discussing various detection techniques such as spectrophotometric detection, electrochemical detection and other less common techniques. It then covers in detail an array of related topics and numerous applications. It is an essential text for anyone exploring the myriad possibilities of this rapidly expanding field.

Clinical Applications of Capillary Electrophoresis

Автор: Terry M. Phillips; Heather Kalish
Название: Clinical Applications of Capillary Electrophoresis
ISBN: 1493962388 ISBN-13(EAN): 9781493962389
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This Methods in Molecular Biology (TM) book is an in-depth manual of Capillary Electrophoresis applications in important areas of clinical science, including clinical chemistry, hematology, disease-associated biomarker discovery, immunology and genetic analysis.

DNA Electrophoresis

Автор: Svetlana Makovets
Название: DNA Electrophoresis
ISBN: 1493959980 ISBN-13(EAN): 9781493959983
Издательство: Springer
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Цена: 14673.00 р.
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Описание: This book details methods used to study DNA dynamics both in live cells and in test tubes. It features step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Pulsed-Field Gel Electrophoresis

Автор: Margit Burmeister; Levy Ulanovsky
Название: Pulsed-Field Gel Electrophoresis
ISBN: 1489943919 ISBN-13(EAN): 9781489943910
Издательство: Springer
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Цена: 6986.00 р.
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Capillary Electrophoresis of Carbohydrates

Автор: Pierre Thibault; Susumu Honda
Название: Capillary Electrophoresis of Carbohydrates
ISBN: 1617372064 ISBN-13(EAN): 9781617372063
Издательство: Springer
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Цена: 20263.00 р.
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DNA Electrophoresis Protocols for Forensic Genetics

Автор: Antonio Alonso
Название: DNA Electrophoresis Protocols for Forensic Genetics
ISBN: 1493962191 ISBN-13(EAN): 9781493962198
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This volume in the Methods in Molecular Biology (TM) series covers methods in forensic DNA profiling, including protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2.


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