Adult and Pluripotent Stem Cells, J?rgen Hescheler; Erhard Hofer

Автор: Uma Lakshmipathy; Mohan C Vemuri
Название: Pluripotent Stem Cells
ISBN: 149396285X ISBN-13(EAN): 9781493962853
Издательство: Springer
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Описание: Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific or disease-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods , and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

Автор: Kaiming Ye; Sha Jin
Название: Human Embryonic and Induced Pluripotent Stem Cells
ISBN: 1493958933 ISBN-13(EAN): 9781493958931
Издательство: Springer
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Цена: 25155.00 р.
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Описание: Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world. The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies. Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.

Автор: Philip H. Schwartz; Robin L. Wesselschmidt
Название: Human Pluripotent Stem Cells
ISBN: 149395802X ISBN-13(EAN): 9781493958023
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This authoritative book is as an ideal guide to scientists conducting their own pluripotent cell research programs. The book aims to assist those making strides in this field towards furthering human knowledge and, ultimately, improving the human condition.

Автор: Michal Amit; Joseph Itskovitz-Eldor
Название: Atlas of Human Pluripotent Stem Cells
ISBN: 1493957864 ISBN-13(EAN): 9781493957866
Издательство: Springer
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Цена: 19589.00 р.
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Описание: This comprehensive atlas offers step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions and in non-adhesion suspension culture, as well as methods for examining pluripotency and karyotype stability.

Автор: Kursad Turksen; Andras Nagy
Название: Induced Pluripotent Stem (iPS) Cells
ISBN: 1493930540 ISBN-13(EAN): 9781493930548
Издательство: Springer
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Цена: 22359.00 р.
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Автор: Uma Lakshmipathy; Mohan C Vemuri
Название: Pluripotent Stem Cells
ISBN: 1627033475 ISBN-13(EAN): 9781627033473
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This Methods in Molecular Biology (TM) book discusses iPSC production from diverse somatic sources, commonly used characterization methods and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering.

Автор: Andras Nagy; Kursad Turksen
Название: Patient-Specific Induced Pluripotent Stem Cell Models
ISBN: 1493930338 ISBN-13(EAN): 9781493930333
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases.

Автор: Andras Nagy; Kursad Turksen
Название: Patient-Specific Induced Pluripotent Stem Cell Models
ISBN: 149394973X ISBN-13(EAN): 9781493949731
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases.

Автор: Xiaoyang Zhao
Название: Studies of pluripotency in embryonic stem cells and induced
ISBN: 9402402268 ISBN-13(EAN): 9789402402261
Издательство: Springer
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Цена: 13059.00 р.
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Описание: This book covers methods to derive embryonic stem cells (ESCs) from several different species. It describes an efficient system to generate induced pluripotent stem cells (iPSCs) and reports on iPSC mice.

Автор: Xiaoyang Zhao
Название: Studies of pluripotency in embryonic stem cells and induced
ISBN: 9401788189 ISBN-13(EAN): 9789401788182
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This book covers methods to derive embryonic stem cells (ESCs) from several different species. It describes an efficient system to generate induced pluripotent stem cells (iPSCs) and reports on iPSC mice.

Автор: John R. Masters; Bernhard ?. Palsson (Eds.)
Название: Human Adult Stem Cells
ISBN: 9048122686 ISBN-13(EAN): 9789048122684
Издательство: Springer
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Цена: 32004.00 р.
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Описание: The aim of volume 7 of "Human Cell Culture" is to provide clear and precise methods for growing primary cultures of adult stem cells from various human tissues and describe culture conditions in which these adult stem cells differentiate along their respective lineages. The book will be of value to biomedical scientists and of special interest to stem cell biologists and tissue engineers. Each chapter is written by experts actively involved in growing human adult stem cells.

Автор: Turksen
Название: Adult Stem Cells. 2004
ISBN: 1588291529 ISBN-13(EAN): 9781588291523
Издательство: Springer
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Цена: 28734.00 р.
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Описание: 2-D Protein Gel Electrophoresis: An Overview, Jenny Fichmann and Reiner Westermeier. Solubilization of Proteins in 2-D Electrophoresis: An Outline, Thierry Rabilloud. Preparation of Escherichia coli Samples for 2-D Gel Analysis, Ruth A. VanBogelen and Frederick C. Neidhardt. Preparing 2-D Protein Extracts from Yeast, David M. Schieltz. 2-D Protein Extracts from Drosophila melanogaster, Christer Ericsson. Preparing 2-D Protein Extracts from Caenorhabditis elegans, Robert Zwilling. Eukaryotic Cell Labeling and Preparation for 2-D, Nick Bizios. Differential Detergent Fractionation of Eukaryotic Cells: Analysis by Two-Dimensional Gel Electrophoresis, Melinda L. Ramsby and Gregory S. Makowski. Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis, Joachim Klose. Preparation and Solubilization of Body Fluids for 2-D, Jean Charles Sanchez and Denis F. Hochstrasser. 2-D Electrophoresis of Plant Proteins, Akira Tsugita and Masaharu Kamo. Quantifying Protein in 2-D PAGE Solubilization Buffers, Louis S. Ramagli. Measuring the Radioactivity of 2-D Protein Extracts, Andrew J. Link and Nick Bizios. Advantages of Carrier Ampholyte IEF, Mary F. Lopez. 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF), Mary F. Lopez. Nonequilibrium pH Gel Electrophoresis (NEPHGE), Mary F. Lopez. High Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment, Marion Sarmiento. Large-Gel 2-D Electrophoresis, Joachim Klose. Advantages of Immobilized pH Gradients, Jenny Fichmann. Casting Immobilized pH Gradients (IPGs), Elisabetta Gianazza. Analytical IPG-Dalt, Angelika GГ¶rg and Walter Weiss. IPG-Dalt of Very Alkaline Proteins, Angelika GГ¶rg. Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D, Neil M. Matsui, Diana M. Smith-Beckerman, Jenny Fichmann, and Lois B. Epstein. In-Gel Sample Rehydration of Immobilized pH Gradient, Jean-Charles Sanchez, Denis Hochstrasser, and Thierry Rabilloud. High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis, Jean-Charles Sanchez and Denis F. Hochstrasser. Horizontal SDS-PAGE for IPG-Dalt, Angelika GГ¶rg and Walter Weiss. Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE, Bradley J. Walsh and Benjamin R. Herbert. Nonreducing 2-D Polyacrylamide Gel Electrophoresis, Hong Ji and Richard J. Simpson. 2-D Diagonal Gel Electrophoresis, Joan Goverman. 2-D Phosphopeptide Mapping, Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky, and Steven F. Dowdy. Internal Standards for 2-D, Andrew J. Link. Autoradiography of 2-D Gels, Andrew J. Link. Double-Label Analysis, Kelvin H. Lee and Michael G. Harrington. Silver Staining of 2-D Electrophoresis Gels, Thierry Rabilloud. Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining, Neil M. Matsui, Diana M. Smith-Beckerman, and Lois B. Epstein. Electroblotting of Proteins from 2-D Polyacrylamide Gels, Michael J. Dunn. Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels, Michael J. Dunn. Protein Detection Using Reversible Metal Chelate Stains, Wayne F. Patton, Mark J. Lim, and David Shepro. Gylcoprotein Detection of 2-D Separated Proteins, Nicolle H. Packer, Malcolm S. Ball, and Peter L. Devine. Image Acquisition in 2-D Electrophoresis, Wayne F. Patton, Mark J. Lim, and David Shepro. Computer Analysis of 2-D Images, Ron D. Appel and Denis F. Hochstrasser. 2-D Databases on the World Wide Web, Ron D. Appel, Amos Bairoch, and Denis F. Hochstrasser. Comparing 2-D Electrophoresis Gels Across Internet Databases, Peter F. Lemkin. Constructing a 2-D Database for the World Wide Web, Ron D. Appel, Christine Hoogland, Amos Bairoch, and Denis F. Hochstrasser. Absolute Quantitation of 2-D Protein Spots, Steven P. Gygi and Ruedi Aebersold. Generating a Bacterial Genome Inventory: Identifying 2-D Spots by Comigrating Products of the Genome on 2-D Gels, Ruth A. VanBogelen. Immunoaffinity Identification of 2-DE Separated Proteins, Barbara Magi, Luca Bini, Barbara Marzocchi, Sabrina Liberatori, Roberto Raggiaschi, and Vitaliano Pallini. 2-DE Spot Amino Acid Analysis with 9-Fluroenylmethyl Chloroformate, Marc R. Wilkins, Jun X. Yan, and Andrew A. Gooley. N-Terminal Amino Acid Sequencing of 2-DE Spots, Masaharu Kamo and Akira Tsugita. Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategies for Microsample Handling, Hediye Erdjument-Bromage, Mary Lui, Lynne Lacomis, and Paul Tempst. Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry, Rachel R. Ogorzalek Loo, Joseph A. Loo, and Philip C. Andrews. Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses, Paul L. Courchesne and Scott D. Patterson. Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Protein Identification and Analysis Tools in the ExPASy Server, Marc R. Wilkins, Elisabeth Gasteiger, Amos Bairoch, Jean-Charles Sanchez, Keith L. Williams, Ron D. Appel, and Denis F. Hochstrasser. Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry, John R. Yates III, Edwin Carmack, Lara Hays, Andrew J. Link, and Jimmy K. Eng. Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry, Ole NГёrregaard Jensen, Matthias Wilm, Andrej Shevchenko, and Matthias Mann. Index.