Biotechnologies for Plant Mutation Breeding, Joanna Jankowicz-Cieslak; Thomas H. Tai; Jochen Ku

Mutagenesis for Crop Breeding and Functional Genomics.- Chemical and Physical Mutagenesis in Jatropha curcas.- Chemical Mutagenesis and Chimera Dissolution in Vegetatively Propagated Banana.- Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.).- Optimization of Somatic Embryogenesis in Cassava.- Creation of a TILLING Population in Barley after Chemical Mutagenesis with Sodium Azide and MNU.- Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen.- Doubled Haploidy as a Tool for Chimera Dissolution of TALEN-Induced Mutations in Barley.- Field Evaluation of Mutagenized Rice Material.- Root Phenotyping Pipeline for Cereal Plants.- Breeding New Aromatic Rice with High Iron using Gamma Radiation and Hybridization.- Utilizing NIRS for Qualitative and Non-Destructive Identification of Seed Mutants in Large Populations.- Protocols for Proteome Analyses of Jatropha curcas.- Low-Cost Methods for DNA Extraction and Quantification.- A Protocol for Benchtop Extraction of Single-Strand-Specific Nucleases for Mutation Discovery.- A Protocol for Validation of Doubled Haploid Plants by Enzymatic Mismatch Cleavage.- Bioinformatics-Based Assessment of the Relevance of Candidate Genes for Mutation Discovery.- Mutation Detection by Analysis of DNA Heteroduplexes in TILLING Populations of Diploid Species.- Determining Mutation Density using Restriction Enzyme Sequence Comparative Analysis (RESCAN).- Next-Generation Sequencing for Targeted Discovery of Rare Mutations in Rice.