Interferon Methods and Protocols, Daniel J. J. Carr

Автор: W.E.G. M?ller; H.C. Schr?der
Название: Biological Response Modifiers — Interferons, Double-Stranded RNA and 2?,5?-Oligoadenylates
ISBN: 3642785514 ISBN-13(EAN): 9783642785511
Издательство: Springer
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Описание: Biological response modifiers are increasingly used in viral and cancer therapy. Since alterations of the immune system are the primary symptoms of HIV infection, especially therapies directed towards the modulation of the immune response have been under intense evaluation.

Автор: Proetzel
Название: Antibody Methods and Protocols
ISBN: 1617799300 ISBN-13(EAN): 9781617799303
Издательство: Springer
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Описание: The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.  Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.  The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.

Автор: Andras Perl (Editor)
Название: Autoimmunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1607617196 ISBN-13(EAN): 9781607617198
Издательство: Springer
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Описание: Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.

Автор: Ludewig Burkhard, Hoffmann Matthias W.
Название: Adoptive Immunotherapy / Methods and Protocols
ISBN: 1588294064 ISBN-13(EAN): 9781588294067
Издательство: Springer
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Описание: An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Автор: Kerry S. Campbell
Название: Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology, Vol. 612)
ISBN: 1607613611 ISBN-13(EAN): 9781607613619
Издательство: Springer
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Описание: Research has revealed that natural killer (NK) cells are sentinels of the innate immune response. This text offers detailed protocols plus the latest methods and techniques designed to study NK cells within specialized tissue sites in both mice and humans.

Автор: Gwyn Davies (Editor)
Название: Vaccine Adjuvants: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1607615843 ISBN-13(EAN): 9781607615842
Издательство: Springer
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Описание: The study of vaccine adjuvants has developed into an exciting area of work. In this authoritative and versatile book, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products.

Автор: Irving C. Allen (Editor)
Название: Mouse Models of Innate Immunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034803 ISBN-13(EAN): 9781627034807
Издательство: Springer
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Цена: 20962.00 р.
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Описание: The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense;

Автор: Andrea A. Zachary (Editor), Mary S. Leffell
Название: Transplantation Immunology: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034927 ISBN-13(EAN): 9781627034920
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Описание: PART I: Overview Chapters1. Alloreactivity

Sidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey

2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury

Nicole M. Valenzuela and Elaine F. Reed

3. Cell Mediated Rejection

Kathryn J Wood, Masaaki Zaitsu and Ryoichi Goto

4. Transplantation Tolerance

Colin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg

5. Composite Tissue Transplantation

Gerald Brandacher

6. Transplantation of the Sensitized Patient: Histocompatibility Testing

Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary

7. Thoracic Organ Transplantation: Laboratory Methods

Jignesh K. Patel and Jon A. Kobashigawa

PART II: Methods Chapters

8. HLA Typing by Sequence Specific Primers

Mike Bunce, Biofortuna and Ben Passey, Biofortuna

9. Human Leukocyte Antigen (HLA) Typing By DNA Sequencing

Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley

10. Next-Generation HLA Sequencing using the 454 GS FLX System

Elizabeth A. Trachtenberg and Cherie L. Holcomb

11. Chimerism Testing by Quantitative PCR Using Indel Markers

Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer

12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR)

Raja Rajalingam and Elham Ashouri

13. Complement-dependent Cytotoxicity Crossmatch

Jeremy Ryan Peсa, Donna Fitzpatrick, and Susan L. Saidman

14. Lymphocyte Crossmatching by Flow Cytometry

Robert A. Bray

15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies

John D Smith and Marlene L Rose

16. C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens

Ge Chen and Dolly B. Tyan

17. Tetramer Staining for the Detection of HLA-specific B cells

Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary

18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood

Annette M. Jackson, Donna P. Lucas, Jessica L. Badders

19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation

Duska Dragun

20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA)

Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar

21. Cylex ImmuKnow Cell Function Assay

Adriana Zeevi and John Lunz

22. Detection of Intracellular Cytokines

Nancy L. Reinsmoen and Chih-Hung Lai

23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients

George Vlad and Nicole Suciu-Foca

24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA

Tara K. Sigdel, and Minnie. M. Sarwal

25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing

Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian

Автор: Carr Daniel J. J.
Название: Interferon Methods and Protocols
ISBN: 1588294188 ISBN-13(EAN): 9781588294180
Издательство: Springer
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Цена: 16769.00 р.
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Описание: A compendium of methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. This book offers researchers tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of various biological events influenced by IFNs.

Автор: Paula M. Pitha
Название: Interferon: The 50th Anniversary
ISBN: 3642090443 ISBN-13(EAN): 9783642090448
Издательство: Springer
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Цена: 28732.00 р.
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Описание: A vital text for researchers and clinicians in the fields of virology and oncology alike, this book is a comprehensive guide to one of medicine`s most important tools that covers the most recent research. Important insights have been made in recent months that are challenging the accepted concept of the critical role of dsRNA in IFN induction.

Автор: J. Lindenmann; W.-D. Schleuning
Название: Interferon: The Dawn of Recombinant Protein Drugs
ISBN: 3662037890 ISBN-13(EAN): 9783662037898
Издательство: Springer
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Цена: 16979.00 р.
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Описание: Forty years of Interferon I wish to dedicate this short introduction to the memory of Alick Isaacs (1921-1967), and to that of Sir Christopher Andrewes (1896-1988). He was struck by the following observation: if a suitable chicken broth was inoculated with the bacterium, the organism grew profusely and the liquid became tur- bid.

Автор: V. Solov`ev
Название: Interferon
ISBN: 146842033X ISBN-13(EAN): 9781468420333
Издательство: Springer
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Описание: Work on the mechanism of interferon induction, the relative role of the cellular constituents, and work in cell-free systems and the molecular mechanism of the antiviral action of interferon continues to excite scientists in the field.