Yeast Protocols, Wei Xiao

Автор: Xiao Wei
Название: Yeast protocols
ISBN: 1588294374 ISBN-13(EAN): 9781588294371
Издательство: Springer
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Описание: In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

Автор: John F.T. Spencer; Dorothy M. Spencer; I.J. Bruce
Название: Yeast Genetics
ISBN: 3540188053 ISBN-13(EAN): 9783540188056
Издательство: Springer
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Описание: The manual consists of two main sections. The first includes the essential, sometimes laborious, procedures for handling yeasts, for inducing mating and isolation of hybrids, for inducing sporulation and isolation of single-spore clones, with some details of tetrad analysis, and including techniques and ancillary equipment for use of the micromanipulator. There are also proce- dures for induction of mutants by physical and chemical agents, and for iso- lation of particular types of mutants, such as to temperature sensitivity, for increased frequency of mutations, for mutations in the mitochondrial genome, both to the petite colonie form and to resistance to antibiotics, for mutations in that part of the yeast genome controlling the glycolytic cycle, and numerous others. Mapping of mutations is discussed briefly, though this aspect of yeast genetics is probably one which should not be undertaken until the investigator has gained a certain amount of experience in the field. How- ever, as is pointed out in the pertinent part of the manual, the task of mapping has been tremendouslysimplified by the availability from the Yeast Genetics Stock Center at the University of California at Berkeley of a set of auxo- trophic strains designed to permit mapping of most unknown genes with a minimum number of crosses and tetrad analyses. The first section concludes with the description of methods for hybridization of yeasts by protoplast fusion, which has been described as the poor man's system for genetic engineering.

Автор: Tulasi Satyanarayana; Gotthard Kunze
Название: Yeast Diversity in Human Welfare
ISBN: 9811026203 ISBN-13(EAN): 9789811026201
Издательство: Springer
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Описание: This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind.

Автор: Alistair J.P. Brown; Mick F. Tuite; John E.G. McCa
Название: Protein Synthesis and Targeting in Yeast
ISBN: 3642849237 ISBN-13(EAN): 9783642849237
Издательство: Springer
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Цена: 16979.00 р.
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Описание: Proceedings of the NATO Advanced Research Workshop on Protein Synthesis and Targeting in Yeast, held at Canterbury, Kent, UK, July 4-8, 1992

Автор: Michael Breitenbach; S. Michal Jazwinski; Peter La
Название: Aging Research in Yeast
ISBN: 9400794207 ISBN-13(EAN): 9789400794207
Издательство: Springer
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Описание: Collecting current research on yeast aging, this book covers mitochondrial theories of aging, emphasizing oxidative stress and retrograde responses; autophagy and mitophagy; the relationship of apoptosis to aging processes; replication stress and more.

Автор: Yuko Giga-Hama; Hiromichi Kumagai
Название: Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe
ISBN: 3662034743 ISBN-13(EAN): 9783662034743
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.