Описание: 1. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format
Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov
2. Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry
Xin Ying Lim, Russell Pickford, and Anthony S Don
3. Fluorescent Assays for Ceramide Synthase Activity
Timothy A Couttas and Anthony S Don
4. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha
Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, and Victor Faundez
5. Measurement of Long Chain Fatty Acyl-CoA synthetase Activity
Joachim Fьllekrug and Margarete Poppelreuther
6. Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity
Laura Ren Huey Ip and Christina Anja Gewinner
7. Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity
Matthew C. Gage, Benoit Pourcet, and Inйs Pineda-Torra
8. Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains
Kimberly J. Krager and John G. Koland
9. Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell
MembranesMitul Sarkar and John G. Koland
10. Isolation and Analysis of Detergent-Resistant Membrane Fractions
Massimo Aureli, Sara Grassi, Sandro Sonnino, and Alessandro Prinetti
11. Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 Receptor
Abasha Lewis and Tsung-Ping Su
12. Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions
Kathryn Del Vecchio and Robert V. Stahelin
13. Analyzing Protein-phosphoinositide Interactions with Liposome Flotation Assays
Ricarda A. Busse, Andreea Scacioc, Amanda M. Schalk, Roswitha Krick, Michael Thumm, and Karin Kьhnel
14. High-Throughput Flurometric Assay for Membrane-Protein Interaction
Wonhwa Cho, Hyunjin Kim, and Yusi Hu
15. Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging
Matthieu Pierre Platre and Yvon Jaillais
16. Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]serine
Neale D. Ridgway
17. Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells
Krista Rombouts and Vinicio Carloni
18. Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions
Deborah A. Sarkes and Lucia E. Rameh
19. Single-molecule
Imaging of Signal Transduction via GPI-anchored ReceptorsKenichi G. N. Suzuki
20. Measuring Phosphatidylinositol Generation on Biological Membranes
Mark G. Waugh
21. Assay for CDP-diacylglycerol Generation by CDS in Membrane Fractions
Mark G. Waugh
