Gene Expression Profiling, Richard A. Shimkets

Автор: Balbas Paulina, Lorence Argelia
Название: Recombinant Gene Expression / Reviews and Protocols
ISBN: 1588292622 ISBN-13(EAN): 9781588292629
Издательство: Springer
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Цена: 27950.00 р.
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Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Автор: Rani
Название: MicroRNA Profiling
ISBN: 1493965220 ISBN-13(EAN): 9781493965229
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

Автор: Thomas O. Metz
Название: Metabolic Profiling
ISBN: 149395816X ISBN-13(EAN): 9781493958160
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This book presents protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. It features cutting-edge protocols, presented from the perspective of leading experts in the field.

Автор: Patrick J. Lincoln
Название: Forensic DNA Profiling Protocols
ISBN: 161737038X ISBN-13(EAN): 9781617370380
Издательство: Springer
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Цена: 24456.00 р.
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Описание: It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis.