Animal Cell Electroporation and Electrofusion Protocols, Jac A. Nickoloff

Автор: Jac A. Nickoloff
Название: Plant Cell Electroporation And Electrofusion Protocols
ISBN: 1489940804 ISBN-13(EAN): 9781489940803
Издательство: Springer
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Описание: Gene transfer is an essential technology for improving our under- standing of gene structure and function. Although there are many meth- ods by which DNA may be introduced into cells--including heat and chemical treatments, and microinjection--electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi- ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in- cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna- tives to electric field-based methods, citing the advantages and limita- tions of the various methods for achieving specific goals.

Автор: C.A. Jordan; E. Neumann; A.E. Sowers
Название: Electroporation and Electrofusion in Cell Biology
ISBN: 1489925309 ISBN-13(EAN): 9781489925305
Издательство: Springer
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Описание: The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material.

Автор: Miklavcic
Название: Handbook of Electroporation
ISBN: 3319328859 ISBN-13(EAN): 9783319328850
Издательство: Springer
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Описание: This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment.

Автор: Boris Rubinsky
Название: Irreversible Electroporation
ISBN: 3642054196 ISBN-13(EAN): 9783642054198
Издательство: Springer
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Цена: 26122.00 р.
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Описание: Written by leading researchers, this book provides a brief introduction to the emerging field of irreversible electroporation in medicine. A brief historical perspective of irreversible electroporation is presented and recent studies are discussed.

Автор: Boris Rubinsky
Название: Irreversible Electroporation
ISBN: 364226199X ISBN-13(EAN): 9783642261992
Издательство: Springer
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Цена: 23508.00 р.
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Описание: Written by leading researchers, this book provides a brief introduction to the emerging field of irreversible electroporation in medicine. A brief historical perspective of irreversible electroporation is presented and recent studies are discussed.