Описание: Part I: Lung Innate, Immunity, and Inflammation
1. 500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy
William McKleroy and Kenneth Lyn-Kew
2. Overview of Innate Lung Immunity and Inflammation
David W.H. Riches and Thomas R. Martin
Part II: Isolation and Characterization of Lung Innate Immune Cells
3. Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes
Sophie L. Gibbings and Claudia V. Jakubzick
4. Isolation and Characterization of Mouse Neutrophils
Niki D.J. Ubags and Benjamin T. Suratt
5. Isolation and Characterization of Mouse Fibroblasts Benjamin L. Edelman and Elizabeth F. Redente
6. Isolation of Rat and Mouse Alveolar Type II Epithelial Cells
Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok, and Rachel L. Zemans
7. Isolation and Characterization of Human Alveolar type II Cells
Beata Kosmider, Robert J. Mason, and Karim Bahmed
8. Air-liquid Interface Culture of Human and Mouse Airway Epithelial Cells Di Jiang, Niccolette Schaefer, and Hong Wei Chu
9. Isolation and Characterization of Human Lung Myeloid Cells
Yen-Rei A. Yu and Robert M. Tighe
10. Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells under Pathological Conditions
Xiaohui Fang and Michael A. Matthay
11. Measuring Innate Immune Function in Mouse Mononuclear Phagocytes John Matthew Craig and Neil Raj Aggarwal
12. Measuring Neutrophil Bactericidal Activity
Kenneth C. Malcolm
13. Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches
Alexandra L McCubbrey and William J. Janssen
14. Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches
Adrianne L. Stefanski, Dorota S. Raclawska, and Christopher M. Evans
15. Computational Analysis of RNA-seq Data from Airway Epithelial Cells for Studying Lung Disease
Nathan D. Jackson, Lando Ringel, and Max A. Seibold
16. Application of Proteomics in Lung Research Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, and Richard Reisdorph
17. Application of Metabolomics in Lung Research
Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, and Richard Reisdorph
18. Functional Genomics in Murine Macrophages
Frank Fang-Yao Lee and Scott Alper
Part III: Analysis of Lung Innate Immunity and Inflammation
19. Assessment of Ozone-induced Lung Injury in Mice
Vandy P. Stober and Stavros Garantziotis
20. Lung Histological Methods
Aneta Gandjeva and Rubin M. Tuder
21. Intravital Microscopy in the Mouse Lung
Yimu Yang, Joseph A Hippensteel, and Eric P Schmidt
22. Mouse Models of Acute Lung Injury and ARDS
Franco R. DAlessio
23. Mouse Models of Asthma Magdalena M. Gorska
24. Animal Models of Pulmonary Fibrosis
David N. ODwyer and Bethany B. Moore
25. Mouse Models of COPD
Karina A. Serban and Irina Petrache
26. Mouse Models of COPD
Karina A. Serban and Irina Petrache
27. Delivery of Therapeutics to the L
