T-cell motility 9781493990351

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Название: T-cell motility
ISBN: 9781493990351
Издательство: Springer
Классификация:

Исследование рынка

Иммунология

Биохимия

Биохимическая иммунология

Клеточная биология (цитология)

ISBN-10: 1493990357
Обложка/Формат: Hardcover
Страницы: 158
Вес: 0.56 кг.
Дата издания: 05.01.2019
Серия: Methods in molecular biology
Язык: English
Издание: 1st ed. 2019
Иллюстрации: 27 tables, color; 28 illustrations, color; 2 illustrations, black and white; viii, 132 p. 30 illus., 28 illus. in color.
Размер: 186 x 263 x 18
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание:

Preface...
Table of Contents...
Contributing Authors...

1. An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility
Navin Kumar Verma and Dermot Kelleher

2. Isolation of Human Peripheral Blood T Lymphocytes
Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma

3. A Laboratory Model to Study T-Cell Motility
Navin Kumar Verma, Sjyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher

4. Quantification of T-Cell Migratory Phenotypes using High Content Analysis
Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma

5. Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time
Seow Theng Ong and Navin Kumar Verma

6. Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells
Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma

7. Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis
Praseetha Prasannan and Navin Kumar Verma

8. Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes
Atish Kizhakeyil and Navin Kumar Verma

9. GapmeR-Mediated Gene Silencing in Motile T-Cells
Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Chalasani, Atish Kizhakeyil, and Navin Kumar Verma

10. Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy
Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann

11. Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes
Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma

12. Immunometabolomic Phenotyping of Motile T-Cells
Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma

13. Profiling Activity of Cellular Kinases in Migrating T-Cells
Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and Wim Vanden Berghe

14. Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes
Malin Samuelsson and Lena M. Svensson

15. A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy
Mobashar Hussain Urf Turabe Fazil, Pankal Kumar, and Navin Kumar Verma

16. Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides
Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma

17. Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility
Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers

18. Computational Analysis of Protein-Protein Interactions in Motile T-Cells
Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khursheed Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma

Дополнительное описание:

An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility.- Isolation of Human Peripheral Blood T Lymphocytes.- A Laboratory Model to Study T-Cell Motility.- Quantification of T-Cell Migratory Phenotypes using High Content Analysis.- Live Cell

T-cell motility,