P-Type ATPases, Maike Bublitz

Название: P-type atpases
ISBN: 1493931784 ISBN-13(EAN): 9781493931781
Издательство: Springer
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Описание:

1. An Introduction to P-type ATPase research

Poul Nissen

Part I: Protein Production, Isolation, Purification, and Stabilization

2. Purification of Na, K-ATPase from Pig Kidney

Natalya U. Fedosova

3. Preparation of Ca²⁺-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum

Jesper V. M ller and Claus Olesen

4. Isolation of H⁺/K⁺-ATPase-enriched Membrane Fraction from Pig Stomachs

Kazuhiro Abe and Claus Olesen

5. Overproduction of PiB-type ATPases

Xiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon

6. Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex

Hassina Azouaoui, Cйdric Montigny, Aurore Jacquot, Raphaлlle Barry, Philippe Champeil, and Guillaume Lenoir

7. The Plasma Membrane Ca²⁺-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein

Verena Niggli and Ernesto Carafoli

8. Expression of Na, K-ATPase and H, K-ATPase Isoforms With The Baculovirus Expression System

Jan B. Koenderink and Herman G.P. Swarts

9. Time-dependent Protein Thermostability Assay

Ilse Vandecaetsbeek and Peter Vangheluwe

Part II: Activity Assays

10. Colorimetric Assays of Na, K-ATPase

Kathleen J. Sweadner

11. ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay Pankaj Sehgal, Claus Olesen, and Jesper V. M ller

12. Antimony-Phosphomolybdate ATPase Assay

Gianluca Bartolommei and Francesco Tadini-Buoninsegni

13. ATPase Activity Measurements using radiolabeled ATP

Herman G.P. Swarts and Jan B. Koenderink

14. Assaying P-type ATPases Reconstituted in Liposomes

Hans-Jьrgen Apell and Bojana Damnjanovic

15. Coupling ratio for Ca²⁺ Transport by Calcium Oxalate Precipitation

Pankaj Sehgal, Claus Olesen, and Jesper V. M ller

16. Calcium Uptake in Crude Tissue Preparation

Philip A. Bidwell and Evangelia G. Kranias

17. Measuring H⁺ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems

Автор: Sajal Chakraborti; Naranjan S Dhalla
Название: Regulation of Membrane Na+-K+ ATPase
ISBN: 3319247484 ISBN-13(EAN): 9783319247489
Издательство: Springer
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Описание: Na+-K+ ATPase or Na-pump ATPase, a member of “P”-type ATPase superfamily, is characterized by association of multiple isoforms mainly of it’s ?- and ?- subunits. At present four different ?- (?-1,?-2,?-3 and ?-4) and three ?- (?-1, ?-2, and ?-3) isoforms have been identified in mammalian cells and their differential expressions are tissue specific. Regulation of Na+-K+ ATPase activity is an important but a complex process, which involves short-term and long-term mechanisms. Short-term regulation of Na+-K+ ATPase is either mediated by changes in intracellular Na+ concentrations that directly affect the Na+-pump activity or by phosphorylation/dephosphorylation-mediated by some stimulants leading to changes in its expression and transport properties. On the other hand, long-term regulation of Na+-K+ ATPase is mediated by hormones, such as mineralocorticoids and thyroid hormones, which cause changes in the transcription of genes of ?- and ?- subunits leading to an increased expression in the level of Na+-pump. Several studies have revealed a relatively new type of regulation that involves the association of small, single span membrane proteins with this enzyme. These proteins belong to the FXYD family, the members of which share a common signature sequence encompassing the transmembra ne domain adjacent to the isoform(s) of ?-? subunits of Na+-K+ ATPase. Considering the extraordinary importance of Na+-K+ ATPase in cellular function, several internationally established investigators have contributed their articles in the monograph entitled “Regulation of Membrane Na+-K+ ATPase” for inspiring young scientists and graduate students to enrich their knowledge on the enzyme, and we are sure that this book will soon be considered as a comprehensive scientific literature in the area of Na+-K+ ATPase regulation in health and disease.

Автор: Sajal Chakraborti; Naranjan S Dhalla
Название: Regulation of Ca2+-ATPases,V-ATPases and F-ATPases
ISBN: 3319247786 ISBN-13(EAN): 9783319247786
Издательство: Springer
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Описание: The three main types of the ion pump ATPase family are: (i) P-type ATPases that transport different ions across membranes and Ca2+ATPases belongs to this catagory (ii) F-type ATPase in mitochondria, chloroplasts and bacterial plasma membranes produce ATP using the proton gradient;

Автор: Sajal Chakraborti; Naranjan S Dhalla
Название: Regulation of Ca2+-ATPases,V-ATPases and F-ATPases
ISBN: 3319796771 ISBN-13(EAN): 9783319796772
Издательство: Springer
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Цена: 27950.00 р.
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Описание: The biological membranes of cellular organization enfold an important group of membrane proteins called the ATPases, which are not only versatile in maintaining chemical gradient and electrical potential across the membrane but also bring metabolites necessary for cell metabolism and drive out toxins, waste products and solutes that otherwise can curb cell functions. ATPases are distributed virtually in all live forms starting from unicellular to multicellular and also in viruses. There are different types of ATPases, which differ in function and structure and in the type of ions they transport. The three main types of the ion pump ATPase family are: (i) P-type ATPases that transport different ions across membranes and Ca2+ATPases belongs to this catagory (ii) F-type ATPase in mitochondria, chloroplasts and bacterial plasma membranes produce ATP using the proton gradient; and (iii) V-type ATPase catalyzes ATP hydrolysis to transport solutes and maintains acidic pH in organelles like lysosomes. Genetic defects in either of the ATPases cause several diseases and a number of researches have demonstrated the involvement of the members of ATPases in the cell pathology and diseases, thereby penetrating exciting new areas of our understanding. In this book, the authors summarize recent knowledge about the molecular mechanisms associated with Ca2+-ATPase, V-ATPase and F-ATPase in intracellular and extracellular Ca2+ transport, mitochondrial ATP synthase, vesicular H+ transport, and lysosomal pH regulation. This book thereby bridges the gap between fundamental research and biomedical and pharmaceutical applications. The book provides an informative resource to improve ATPase research and modern therapeutic approaches toward different life threatening diseases that are associated with dysregulation of the ATPases.