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Reporter Gene Assays, Robert Damoiseaux; Samuel Hasson


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Цена: 13974.00р.
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При оформлении заказа до: 2025-07-28
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Автор: Robert Damoiseaux; Samuel Hasson
Название:  Reporter Gene Assays
ISBN: 9781493992683
Издательство: Springer
Классификация:
ISBN-10: 1493992686
Обложка/Формат: Soft cover
Страницы: 234
Вес: 0.48 кг.
Дата издания: 2018
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 42 illustrations, color; 11 illustrations, black and white; xii, 234 p. 53 illus., 42 illus. in color.
Размер: Book (Paperback Initiative)
Читательская аудитория: Professional & vocational
Основная тема: Biomedicine
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание:
This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.  Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.


Дополнительное описание:
Genome-Edited Cell Lines for High-Throughput Screening.- High-Throughput Firefly Luciferase Reporter Assays.- Using the 2A Protein Co-Expression System: Multi-Cistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins.- Developing Mamm



Stem Cell Assays

Автор: Mohan C. Vemuri
Название: Stem Cell Assays
ISBN: 1627039376 ISBN-13(EAN): 9781627039376
Издательство: Springer
Рейтинг:
Цена: 27950.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of “transplant-ready” or “clinical-grade” stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, through polymerase chain reaction approaches following transplantation, and more. The scientific progress that makes stem cell biology so exciting also threatens to suffocate researchers with an avalanche of information. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, the book Stem Cell Assays aims to present scientific advances in stem cell methods for a wider use by novice and expert scientists, through the Methods in Molecular Biology series.


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