Mark T. Quinn; Frank R. DeLeo Neutrophil 9781071601532

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Автор: Mark T. Quinn; Frank R. DeLeo
Название: Neutrophil
ISBN: 9781071601532
Издательство: Springer
Классификация:

Медицинская генетика

Иммунология

Клеточная биология (цитология)

ISBN-10: 1071601539
Обложка/Формат: Hardcover
Страницы: 470
Вес: 1.13 кг.
Дата издания: 2020
Серия: Methods in Molecular Biology
Язык: English
Иллюстрации: XVI, 470 p. 86 illus., 49 illus. in color. With online files/update.
Размер: Book w. online files / update
Основная тема: Biomedicine
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.Features•Provides neutrophil protocols that are broadly applicable to leukocyte biology•Includes protocols for isolation of neutrophils from humans and animal species•Presents an interesting and informative overview of the field of neutrophil biology•Facilitates a better understanding of the role of neutrophils in host defenseReadershipMolecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists
Дополнительное описание:

The Role of Neutrophils in the Immune System: An Overview.- Neutrophil Defects and Diagnosis Disorders of Neutrophil Function: An Overview.- Isolation of Human Neutrophils from Venous Blood.- Isolation of Neutrophils from Non-Human Species.- Isolatio

Neutrophil, Mark T. Quinn; Frank R. DeLeo