Bhat Characterization of Plant Viruses 9781071603338

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Автор: Bhat
Название: Characterization of Plant Viruses
ISBN: 9781071603338
Издательство: Springer
Классификация:

Медицинская микробиология и вирусология

Науки о жизни (медико-биологические): общие вопросы

Генетика (немедицинская)

Биология развития

Ботаника и науки о растениях

Физиология растений

Патология и болезни растений

ISBN-10: 1071603337
Обложка/Формат: Hardcover
Страницы: 552
Вес: 1.18 кг.
Дата издания: 2020
Серия: Springer Protocols Handbooks
Язык: English
Издание: 1st ed. 2020
Иллюстрации: 99 illustrations, color; 62 illustrations, black and white; xxi, 552 p. 161 illus., 99 illus. in color.
Размер: 254 x 191 x 25
Читательская аудитория: Professional & vocational
Основная тема: SCL24035
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.
Дополнительное описание: Chapter 1. Glasshouse for maintenance of virus and insect culture.- Chapter 2. Symptoms of virus infected plants.- Chapter 3. Isolation and diagnosis of virus through indicator hosts.- Chapter 4. Host range of viruses.- Chapter 5. Physico-chemical propert

Characterization of Plant Viruses, Bhat