Автор: Greenfield Edward A. Название: Antibodies a Laboratory Manual, Second Edition ISBN: 1936113813 ISBN-13(EAN): 9781936113811 Издательство: Неизвестно Рейтинг: Цена: 24140.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: It has been 25 years since the now-classic Antibodies by Ed Harlow and David Lane appeared. While some things remain the same, many have changed. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include: � antibody purification and storage � engineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis � extensive labeling techniques � new immunoblotting protocols � the latest screening and labeling techniques As Dr. Greenfield notes in his preface to this second edition: "The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand book...the second edition is intended to provide the necessary information and protocols to assist investigators with their first monoclonal antibody effort as well as to provide guidance for more experiences antibody makers who are having some difficulties with a particular project." I have the first edition. Why should I buy the second edition? While the core of the first edition appears in updated and recast form in the second edition, more than half of the second edition contains entirely new information and protocols to reflect the changes in the field since the first edition. To whom is this edition addressed? Is this a book for immunologists? As with the first edition, the second edition provides information and protocols for both the neophyte and the experienced investigator. It dissects the mysteries of producing the antibodies and reagents you need for your experiments with the needs of molecular biologists in mind. It is the perfect book for new members of your lab as it has sufficient background information to provide context as well as clear, step-by-step instructions for performing the needed experiments-both the why and the how for these techniques. And if things go wrong, there is extensive troubleshooting to diagnose the problem. Are the protocols in this book better than those available for download on the Web? Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have.
Автор: Thong Roseanne Название: Green Is a Chile Pepper ISBN: 145215645X ISBN-13(EAN): 9781452156453 Издательство: Abrams Цена: 1102.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In this lively picture book that Booklist described as a cheerful color-concept book that presents a slice of Latino culture through food and fun, children discover a world of colors all around them: red is spices and swirling skirts, yellow is masa, tortillas, and sweet corn cake. Many of the featured objects are Latino in origin, and all are universal in appeal. With rich, boisterous illustrations, fun-to-read rhyming text, and an informative glossary, this playful concept book will reinforce the colors found in every child's day!
Автор: Watt Roseanne Название: Moder Dy ISBN: 1846974879 ISBN-13(EAN): 9781846974878 Издательство: Неизвестно Рейтинг: Цена: 1741.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Written in English, interspersed with Shetlandic dialect throughout, this eagerly awaited debut collection from Shetland poet Roseanne Watt contains profound, assured and wilfully spare poems that are built from the sight, sound and heartbeat of the land as much as from the sea.
Автор: Horton Carol, Harvey Roseanne Название: 21st Century Yoga ISBN: 0615617603 ISBN-13(EAN): 9780615617602 Издательство: Неизвестно Цена: 2586.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Yoga may be rooted in ancient India, but it's morphed into something new in North America today. Precisely what that might be, however, is difficult to say. Yoga is taught everywhere from spas to prisons, and for everything from weight loss to spiritual transcendence. With its chameleon-like ability to adapt equally well to advertising, athletics, and ashrams, contemporary yoga is a fascinating phenomenon that invites investigation. Written by experienced practitioners who are also teachers, therapists, activists, scholars, studio owners, and interfaith ministers, 21st Century Yoga is one of the first books to provide a multi-faceted examination of yoga as it actually exists in the U.S. and Canada today. CONTENTS: Introduction: Yoga and North American Culture - Carol Horton Enlightenment 2.0: The American Yoga Experiment - Julian Walker How Yoga Makes You Pretty: The Beauty Myth, Yoga and Me - Melanie Klein Questioning the "Body Beautiful" Yoga, Commercialism, and Discernment - Poep Sa Frank Jude Boccio Bifurcated Spiritualities: Examining Mind/Body Splits in the North American Yoga and Zen Communities - Nathan Thompson Starved for Connection: Healing Anorexia Through Yoga - Chelsea Roff Yoga and the 12 Steps: Holistic Recovery from Addiction - Tommy Rosen Modern Yoga Will Not Form a Real Culture Until Every Studio Can Also Double as a Soup Kitchen and other observations from the threshold between yoga and activism - Matthew Remski Yoga for War: The Politics of the Divine - Be Scofield Our True Nature is Our Imagination: Yoga and Non-Violence at the Edge of the World - Michael Stone How Yoga Messed With My Mind - Angela Jamison Afterword: The Evolution of Yoga and the Practice of Writing - Roseanne Harvey
Автор: Bennett Roseanne Название: When the Past Calls Your Name ISBN: 1975682416 ISBN-13(EAN): 9781975682415 Издательство: Неизвестно Цена: 2067.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book is a story of intrigue set on the beautiful Outer Banks of North Carolina. Dr. Emmaline Jolie is an advocate for the rights of victims. She is an expert witness and she has a blog that is read by thousands. Someone is trying to kill her and the field is littered with candidates.Retired soldier and cop, Finn Jensen is a reclusive writer who has many secrets and wants to keep them just that...secret. He is writing and expose on the connection between the Department of Defense and the man whom the GOP has tapped to be the next president.Detective Tighe Coker has caught a particularly frustrating murder case in the sleepy bedroom community of Haymarket, Virginia. He is finally able to find a single clue that he hopes will lead him to the killer of Katie Sanders...the dead woman's relationship with Emma Jolie.What happens when these people meet during the storm of the decade, as the clock begins to tick down on a presidential election that someone will do anything to win?
Автор: McDonald Roseanne Название: From a Tree to a Chair ISBN: 1947860763 ISBN-13(EAN): 9781947860766 Издательство: Неизвестно Рейтинг: Цена: 3166.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Look around you. Do you see something made of wood? A chair or a table, a toy or a tool, perhaps even the building you're in right now? Have you ever wondered where these items come from? Prepare to find out Beginning in a busy logging site deep in the woods, we'll follow the journey of a tree as it is harvested, processed into lumber, and carefully crafted into a beautiful set of chairs. With a deep respect for the trees and the useful things they give us, this book reveals all the hard work that goes into making the things we take for granted every day.
Описание: Bomb meets Code Girls in this fascinating narrative nonfiction look at the little-known female scientists who were critical to the invention of the Atomic Bomb, and the moral implications of their work.
The Lady and Her Monsters by Roseanne Motillo brings to life the fascinating times, startling science, and real-life horrors behind Mary Shelley's gothic masterpiece, Frankenstein.
Montillo recounts how--at the intersection of the Romantic Age and the Industrial Revolution--Shelley's Victor Frankenstein was inspired by actual scientists of the period: curious and daring iconoclasts who were obsessed with the inner workings of the human body and how it might be reanimated after death.
With true-life tales of grave robbers, ghoulish experiments, and the ultimate in macabre research--human reanimation--The Lady and Her Monsters is a brilliant exploration of the creation of Frankenstein, Mary Shelley's horror classic.
--The Lady and Her Monsters
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