Описание: Research has revealed that natural killer (NK) cells are sentinels of the innate immune response. This text offers detailed protocols plus the latest methods and techniques designed to study NK cells within specialized tissue sites in both mice and humans.
Автор: Lugli Enrico Название: T-Cell Differentiation: Methods and Protocols ISBN: 1493982338 ISBN-13(EAN): 9781493982332 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
The Vast Universe of T Cell Diversity: Subsets of Memory Cells and their Differentiation.- Basic Aspects of T Helper Cell Differentiation.- FACS Analysis of Memory T lymphocytes.- Intravital Microscopy Analysis of Hepatic T cell dynamics.- Analysis of T Cell Activation by Confocal Microscopy.- Phenotypic and Functional Analysis of Antigen-specific T Cell Exhaustion.- pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel.- Differentiation of Diverse Progenies of Memory T cells from Naпve CD8+ T Cell Precursors.- Gammaretroviral Production and T Cell Transduction To Genetically Retarget Primary T Cells Against Cancer.- Measuring Telomerase Activity in Senescent Human T Cells upon Genetic Modification.- Strategies for T Helper Cell Subset Differentiation from Naпve Precursors.- Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells.- Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation.- Next-generation Sequencing Analysis of Long Non-Coding RNAs in CD4+ T Cell Differentiation.- Highly Multiplexed, Single Cell Transcriptomic Analysis of T-cells by Microfluidic PCR.- Single-cell RNA Sequencing of Human T Cells.- Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry.- Developmental and Functional Assays to Study Murine and Human gd T Cells.
Автор: Ludewig Burkhard, Hoffmann Matthias W. Название: Adoptive Immunotherapy / Methods and Protocols ISBN: 1588294064 ISBN-13(EAN): 9781588294067 Издательство: Springer Рейтинг: Цена: 24456.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.
Автор: Mark T. Quinn; Frank R. DeLeo; Gary M. Bokoch Название: Neutrophil Methods and Protocols ISBN: 1617377791 ISBN-13(EAN): 9781617377792 Издательство: Springer Рейтинг: Цена: 27951.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders.
Автор: B. Paul Morgan Название: Complement Methods and Protocols ISBN: 1617371165 ISBN-13(EAN): 9781617371165 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The complement system, first described more than a century ago, was for many years the ugly duckling of the immunology world, but no more.
Автор: Walter A. Hall Название: Immunotoxin Methods and Protocols ISBN: 1617371831 ISBN-13(EAN): 9781617371837 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Immunotoxins represent a new class of human therapeutics that have widespread applications and a potential that has not yet been fully recognized since they were first conceived of by Paul Ehrlich in 1906.
Автор: Carr Daniel J. J. Название: Interferon Methods and Protocols ISBN: 1588294188 ISBN-13(EAN): 9781588294180 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: A compendium of methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. This book offers researchers tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of various biological events influenced by IFNs.
Автор: Constance Oliver; Maria Celia Jamur (Eds.) Название: Immunocytochemical methods and protocols ISBN: 1588294633 ISBN-13(EAN): 9781588294630 Издательство: Springer Рейтинг: Цена: 9084.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This third edition reflects the rapid evolution of immunocytochemistry in the post-genomic era. Expert researchers explore the latest methods, now widely used in situ to identify components of cells and tissues in both normal and pathological conditions.
Автор: Albitar Maher Название: Monoclonal antibodies / methods and protocols ISBN: 1588295672 ISBN-13(EAN): 9781588295675 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Поставка под заказ.
Описание: Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins. Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.
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