Описание: Part I: Human Immunodeficiency Virus Type 1 (HIV-1)
1. A Fluorescence-Based High Throughput Screening Assay to Identify HIV-1 Inhibitors
Peggy Geluykens, Koen Van Acker, Johan Vingerhoets, Christel Van den Eynde, Marnix Van Loock, and Gйry Dams
2. A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion
Liesbet Smeulders, Lieve Bunkens, Inge Vereycken, Koen Van Acker, Pascale Holemans, Emmanuel Gustin, Marnix Van Loock, and Gйry Dams
3. Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay
Bertrand Van Schoubroeck, Marnix Van Loock, Tania Ivens, Pascale Dehertogh, Dirk Jochmans, and Gйry Dams
4. Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors
Marleen Clynhens, Alexandra Smets, Inge Vereycken, Marnix Van Loock, Reginald Clayton, Geert Meersseman, and Olivia Goethals
5. HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations that Confer Anti-Retroviral Resistance
Peter Van den Eede, Liesbeth Van Wesenbeeck, Yvan Verlinden, Maxim Feyaerts, Veerle Smits, Ann Verheyen, Leen Vanhooren, Alain Deloof, Jorge Villacian, and Theresa Pattery
Part II: Hepatitis Viruses
6. In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis
Rumin Zhang and William T. Windsor
7. A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template
Eric Ferrari and Hsueh-Cheng Huang
8. Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon
Inge Vliegen, Leen Delang, and Johan Neyts
9. A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors
Leen Vijgen, Jannick Verbeeck, Barbara Van Kerckhove, Jan Martin Berke, Diana Koletzki, Gregory Fanning, and Oliver Lenz
10. Expression and Purification of Hepatitis C Virus Protease from Clinical Samples
Liesbet van der Helm
11. Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase
Erna Cleiren, Benoit Devogelaere, and Katleen Fierens
12. Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and the NS5B Polymerase Regions for Genotypic Resistance Detection of Clinical Isolates of Subtypes 1a and 1b
Diana Koletzki, Theresa Pattery, Bart Fevery, Leen Vanhooren, and Lieven J. Stuyver
13. A Southern Blot Assay for Detection of Hepatitis B Virus Covalently Closed Circular DNA from Cell Cultures
Dawei Cai, Hui Nie, Ran Yan, Ju-Tao Guo, Timothy M. Block, and Haitao Guo
14. In Vitro Phenotyping of Recombinant Hepatitis B Virus Containing the Polymerase/Reverse Transcriptase Gene from Clinical Isolates
Yang Liu and Kathryn M. Kitrinos
Part III: Flaviviruses
15. Cell-Based Antiviral Assays for Screening and Profiling Inhibitors Against Dengue Virus
Edwin Yunhao Gong, Marleen Clynhens, Tania Ivens, Pedro Lory, Kenny Simmen, and Guenter Kraus
16. A Duplex Real-Time RT-PCR Assay for Profiling Inhibitors of Four Dengue Serotypes
Edwin Yunhao Gong, Alexandra Smets, Nick Verheyen, Marleen Clynhens, Emmanuel Gustin, Pedro Lory, and Guenter Kraus
17. High Throughput Screening Using Dengue Virus Reporter Genomes
Wolfgang Fischl and Ralf Bartenschlager
18. Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate
Christoph Ni
